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Citations for Leica :
251 - 300 of 425 citations for Sodium hypochlorite solution cp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Cell Biology 2020Quote: 10 μL of the nuclei solution was pipetted on a hemocytometer (Neubauer) for visualization under the (Leica DM IL inverted) microscope ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Neuroscience 2022Quote: ... the apical turn cochlear organs from 1-2 months old Pclowt/wt and Pclogt/gt rats were dissected in ice-cold HEPES Hank’s solution and mounted onto type A specimen carriers (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue blocks were cut coronally with a thickness of 300 μm in this solution using a vibratome (Leica VT1200S). Slices were collected and then incubated in aerated ACSF at 35 °C ...
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 5μm sections were stained with hematoxylin (of Mayer) and 0.2% eosin (ethanolic solution; both Medite) in an automated staining machine (Leica). Ileal and colonic sections were blindly assessed for inflammation (tissue pathology index ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal sections of VISp (320 μm) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21-25°C ...
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bioRxiv - Pathology 2022Quote: ... heat-induced epitope retrieval (HIER) was performed using a ready-to-use citrate-based solution (pH 6.0; Leica Biosystems) at 100°C for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... Stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Cross-sections were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the skull was decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Deer Park, IL). Tissues were then processed in ethanol and xylene ...
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bioRxiv - Cell Biology 2024Quote: ... Organoids were cleared using fructose-glycerol solution for 30 minutes and then imaged TCS SP8 X confocal microscope (Leica).
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bioRxiv - Microbiology 2024Quote: ... tissue sections were subjected to heat-induced epitope retrieval (HIER) using an EDTA-based solution (pH 9.0; Leica Biosystems) at 100°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... and 300 µm coronal sections were taken in an NMDG-based cutting solution using a VT1200S vibratome (Leica Microsystems). Slices containing the BNST were selected and transferred to a chamber containing warmed (34°C) ...
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L1-5) was sectioned transversely (300µm) in dissection solution using a vibrating microtome (Leica Microsystems). Slices were immediately transferred to recording artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then cut into 350 μm transverse slices in the same solution using a vibratome (VT1200S, Leica). The slices were then transferred to recovery aCSF (in mM ...
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bioRxiv - Genomics 2024Quote: ... and once in 95% ethanol before being introduced to alcoholic eosin (Eosin Y Solution, Alcoholic, Cat No. 3801615, Leica). After cytoplasmic staining was completed ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed for 20 min with Bond Epitope Retrieval Solution #2 (Leica Biosystems, equivalent EDTA, pH 9.0). Primary antibody (dilution at 1 ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in an ice-cold choline solution and 300 μm-thick slices were obtained using a vibratome (Leica Biosystems VT1200S). The cutting solution contained the following (in mM) ...
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bioRxiv - Neuroscience 2021Quote: Samples were placed atop the frozen solution and the cryotubes were transferred to an AFS2 freeze substitution unit (Leica Microsystems). Samples were held at -90° for 45 hours ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... and coronal sections (250 μm) containing OFC were cut in ice-cold NMDG solution using a vibratome (Leica, Nussloch, Germany). Slices were then allowed to recover in warm NMDG solution (32°C ...
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bioRxiv - Neuroscience 2021Quote: ... and the cerebellum was cut into 250 μm thick parasagittal slices in the same solution on a vibratome (VT1200S, Leica). Slices were then transferred to 34°C warm artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2021Quote: ... coronal brain slices (250 μm) containing M1 forelimb regions were prepared in the same slicing solution using a vibratome (VT1200S; Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were quickly frozen in embedding solution (Sakura Finetek) and cut into coronal sections using a cryostat (CM3050, Leica) at a thickness of 50 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... IHC technique was performed with commercial solutions from the Novolink™ Max Polymer Detection System Kit (Leica Biosystems, Newcastle UK). Before antibody incubation ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2020Quote: ... Adrenal gland slices were allowed to recover for 15 min at 37°C in bicarbonate-buffered saline in the presence of 0.2 mM (+)-tubocurarine and then kept at room temperature in the same solution before cryofixation in external cryoprotectant (20% bovine serum albumin in bicarbonate-buffered saline) using a HPM100 HPF device (Leica). Freeze substitution was performed as previously published (Rostaing et al. ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 300-μm coronal brain stem slices were prepared in carbonated ice-cold slicing solution using a vibratome (Leica VT 1200S) and allowed to recover for 20 min at 33 °C in carbonated high-osmolarity artificial cerebrospinal fluid (high-Osm ACSF ...
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bioRxiv - Biophysics 2022Quote: ... was mixed quickly with the ATP solution on the grid before blotting for 1 s in an EM GP2 grid freezing device (Leica) at 4 °C and 100% humidity and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Neuroscience 2020Quote: ... The injury section was further cut into 30 μm sections and mounted onto a series of 10 slides coated with Silane Surgipath Solution (Leica). These slides were then stored at −80° C ...
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bioRxiv - Plant Biology 2021Quote: ... The stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Some of the cross-sections were used for FT-IR analysis and the others were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Neuroscience 2021Quote: ... and continuously perfused with HEPES-based Ringer’s solution via am VC 8 perfusion system (Warner Instruments, Cat. No.640186) directly triggered by the recording software (Leica). Images were acquired at 9.8 Hz at a resolution of 512 x 300 pixels using a 488 nm laser in the resonant ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were then decapitated and the brain was isolated in the same sucrose solution and cut on a slicing vibratome (Leica, VT1200S ...
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bioRxiv - Cancer Biology 2022Quote: ... Heat-induced epitope retrieval was performed using citrate-based BOND Epitope Retrieval Solution 1 (Leica Biosystems, Buffalo Grove, IL, USA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and horizontal slices (300 µm) were cut in ice-cold NMDG-based solution using a VT1200 S vibratome (Leica). Slices were then held in NMDG-based solution maintained at 35°C for ∼12 minutes before being transferred to a modified artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cell Biology 2021Quote: ... The tubing was set to allow pumping of the solutions through the heart (attached to a perfusion needle (Leica, 39471024)) into the vasculature with the same entry point used for PBS and PFA perfusion steps described above ...
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bioRxiv - Pathology 2021Quote: ... tissue was subjected to automated baking and deparaffinization followed by heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) at 100 °C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Immunology 2021Quote: ... brains were removed from control and DSS-treated mice and placed in cold Gey’s buffer solution before slicing 200 μm slices using a vibratome (Leica VT1000S). Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed in the same solution and 40 μm-thick coronal slices were cut at the vibratome (Leica). Two PMv coronal sections per animal were selected to perform c-fos immunostaining as follows ...