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Citations for Leica :
351 - 400 of 700 citations for Serum amyloid A 2 protein SAA2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the antigen-antibody binding was detected by Bond Polymer Refine Detection (DS9800, Leica Microsystems) and DAB+ chromogen (DAKO ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibodies were detected using the BOND Polymer Refine Detection System (Leica, DS9800). As positive controls of the IHC ...
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bioRxiv - Immunology 2023Quote: ... Ready-to use secondary antibodies Novolink Post Primary and Novolink Polymer (RE7260-CE, Leica) were used followed by either TSA Cy5 (SAT705A001EA ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (Supplemental Table S1) with a 30M citrate antigen retrieval (Leica ER1 AR9961) were incubated for 30 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... the expression of TiAP1 and TaSYP51 proteins were observed and analysed using a confocal laser-scanning microscope (Leica SP5 - X, Germany) according to the methodology of Smigielski et al ...
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bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
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bioRxiv - Cell Biology 2022Quote: ... Each protein mixture (14 μM for each component) was injected into a homemade chamber and imaged using a Leica microscope (Leica DMi8) using a 40X objective lens ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software). Images were acquired as z-stacks with a z-step size of 120 nm and a X-Y resolution of 37.9 nm ...
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bioRxiv - Cell Biology 2023Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
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bioRxiv - Plant Biology 2024Quote: ... The presence of Cas9 in the shoot apical meristem was investigated by observing the fluorescence of the Cas9-GFP protein (Venus-Cas9) using a confocal laser scanning microscope (TCS SP5, Leica Microsystems). The area of each cell within the SAM was calculated using SEM images of the SAM with Image J software (https://imagej.nih.gov/ij/download.html).
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bioRxiv - Plant Biology 2022Quote: ... the protein fluorescence was confirmed with the Leica TCS LSI macroscope with Plan APO 5x and 20x objectives (Leica Microsystems, Germany), using the settings described previously83 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
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bioRxiv - Microbiology 2024Quote: ... Cellular fluorescence signals in plant leaves agroinfiltrated with BiFC and other constructs expressing fluorescent proteins were observed at 3 dpi using a Leica SP8 laser-scanning confocal microscope (Leica, Germany) equipped with specific laser/filter combinations for YFP (excitation at 514 nm) ...
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bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
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bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
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bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
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bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
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bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
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bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
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bioRxiv - Developmental Biology 2023Quote: ... outside of the necrotic region) (Figure 8-figure supplement 1E-F) from 2 sections with the Application Suite Core v2.4 software (Leica).
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Microbiology 2023Quote: ... Slides were imaged on an inverted Leica TCS SP5 confocal microscope using a 63x/1.40 oil objective with 2-3x digital zoom at calibrated magnifications and recorded with LAS AF software (Leica). Quantitation of dextran and LAMP-1 colocalization with bacteria was analyzed with CellProfiler 3.1.9 (27 ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
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bioRxiv - Biochemistry 2022Quote: ... Plunging was performed at 90-100% humidity via automatic backside blotting (via Leica Microsystems Cryo GP 2 plunge freezer) or dual-side blotting (Mark IV FEI/Thermo Fisher Scientific Vitrobot) ...
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bioRxiv - Neuroscience 2024Quote: ... Olfactory bulbs were dissected and post-fixed in 1% PFA for 2-7 days and embedded in 5% agarose and sectioned into 50µm slices using a vibratome (VT1000S, Leica). Free-floating slices were washed with PBS and incubated in 5% normal goat serum in PBS/Triton/azide (0.25% Triton ...
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bioRxiv - Molecular Biology 2024Quote: ... Images were captured at 2 min 30 sec intervals for 3 hours and processed using LAS X software (Leica).
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and cut into coronal slices (thickness 300 µm, -1.5 mm to 2 mm relative to bregma) on a vibratome (Leica VT1200S ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed for 20 min with Bond Epitope Retrieval Solution #2 (Leica Biosystems, equivalent EDTA, pH 9.0). Primary antibody (dilution at 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... and incubated with an anti-p53 antibody (1:5000; Leica Biosystems; Buffalo Grove, IL, USA) or an anti-GAPDH antibody (1:104 ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody signals were detected on a Leica DM6000 microscope equipped with a DFC390 camera (Leica). Data acquisition was performed with LAS AF (Leica) ...
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bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were diluted as follows: p53 (CM5) (1:500, NCL-L-p53-CM5p, Leica Biosystems), p21 (F-5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL ...
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bioRxiv - Pathology 2021Quote: ... 200 uL of anti-mouse secondary antibody (Leica Cat# PV6114, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... 200 uL of anti-rabbit secondary antibody (Leica Cat# PV6119, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... The antibody information was listed in Supplementary Table 13 and images were taken from Leica TCS SP8 confocal laser-scanning microscope.