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Citations for Leica :
451 - 500 of 700 citations for Serum amyloid A 2 protein SAA2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or 2 hours of 200nM BafA1 before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... They were then embedded in 2% agarose and sliced into coronal sections (70-100µm thick) using a Vibratome (Leica Microsystems). The slices were washed in 0.01M PBS ...
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bioRxiv - Neuroscience 2024Quote: Confocal acquisitions were performed using a Leica SP8 White Light Laser 2 on an inverted stand (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Neuroscience 2024Quote: ... frozen at -80°C for 30 minute then at -20°C for 2 hours before being sectioned using a cryostat (Leica, 50 µm thick coronal sections for immunocytochemistry) ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Cell Biology 2024Quote: ... samples were freeze-substituted at -90°C in anhydrous acetone containing 2% osmium tetroxide and 0.1% uranyl acetate at -90°C for 72 hours and warmed to room temperature with a gradient of +5°C per hour (EM AFS-2, Leica). Sections of the samples were analysed in a Tecnai Spirit T12 Electron microscope equipped with an Eagle OCD camera (Thermo Fisher Scientific).
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bioRxiv - Evolutionary Biology 2024Quote: ... embryos were introduced into an M9 droplet on a 2% agarose pad and immediately imaged on a Leica SP8 confocal on a DM6000 upright microscope (Leica LAS X image acquisition software ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody staining was completed using the Bond Polymer Refine IHC protocol and reagents (Leica, Wetzlar, Germany). Slides were counterstained on the Leica Autostainer XL (Leica ...
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bioRxiv - Genetics 2021Quote: ... Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used: mouse anti-CD45 (Leica Biosystems, NCL-L-LCA, 1/70), mouse anti-CD3 (Leica Biosystems CD3-565-L-CE ...
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bioRxiv - Cancer Biology 2020Quote: ... The TMA slides were sequentially stained with antibodies specific for CD4 (T cells, 4B12, Leica Biosystems), CD8 (T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were applied for 60 mins and detected with Polymer Refine Detection Kit (Leica, DS9800). For detection of P63 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical analyses were performed utilizing ready-to-use Ki67-specific antibody solution (K2, Leica Biosystems, PA0230). Sections were dewaxed ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were diluted as follows: anti-p53 (CM5) (1:500, Leica Biosystems, NCL-L-p53-CM5p), anti-p21 (F-5 ...
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bioRxiv - Immunology 2024Quote: ... Ready to Use Antibody reagent was added as marker and incubated for 15 min (CD4: Leica Bio systems ...
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bioRxiv - Cell Biology 2020Quote: ... Dried samples were then rotary-shadowed with 2 nm of platinum and 5-8 nm of carbon using an ACE600 high vacuum metal coater (Leica Microsystems). Platinum replicas were floated off the glass by 5% hydrofluoric acid ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Bioengineering 2021Quote: ... gravid day 1 adults animals were picked onto prepared palmitic acid plates and plates were imaged at 2 and 5 hours at 1.6x on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Then 3 μL of concentrated hTg were dispensed to the hydrophilic surface of the grid prior to single side blotting for 2 s and plunge freezing in liquid ethane using a Leica EM GP2 plunger (Leica Microsystems) operating at 20 °C and 80% relative humidity.
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bioRxiv - Evolutionary Biology 2022Quote: ... The fixed samples were embedded in Technovit 8100 (Kulzer, Germany) and processed into 2 μm tissue sections using a rotary microtome RM2255 (Leica, Germany). The AlexaFluor555-labeled oligonucleotide probes Eco934 (5’-CAT GCT CCA CCG CTT GTG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Physiology 2021Quote: ... Images were acquired at a rate of 50 Hz with a wide-field fluorescence microscope (DMIRE-2, Leica Microsystems, Wetzlar, Germany) as previously described [24].
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bioRxiv - Immunology 2020Quote: ... The uptake of the fluorescently labeled SVV in IBRS-2 and 293T cells was visualized with a fluorescence microscope (Leica, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type 1 dpf larvae were positioned on top of a 2% agarose plate and imaged using a Leica M165FC dissection scope (Leica Microsystems) with a SPOT RT3 camera (SPOT Imaging) ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Plant Biology 2020Quote: ... mesophyll cells of 2-week-old transgenic Arabidopsis plants expressing Raf36-GFP were observed with a confocal microscope SP8X (Leica Microsystems) with the time-gating method (Kodama ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal slices (300 µm thick) containing visual cortex were prepared using a vibrating blade microtome (Leica VT1200S or Campden 7000smz-2). Slices were immediately transferred to a submerged holding chamber with regular ACSF containing (in mM) ...
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bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
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bioRxiv - Immunology 2021Quote: ... Ultra-thin (50 nm to 60 nm) sections were sectioned with a diamond knife (Jumdi, 2 mm) on an ultramicrotome (Leica Ultracut), contrasted with 2% uranyl acetate (Polyscience ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Developmental Biology 2023Quote: ... GFP images were obtained every 2 s with a x40 oil-immersive objective (HC PL FLUOTAR 40x/1.30 OIL 340 nm, Leica Microsystems CMS) used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...