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Citations for Leica :
351 - 400 of 549 citations for 7 Azaspiro 3.4 octane 6 8 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... For immunohistochemical staining paraffin-embedded brains were cut in 7 μm thick sagittal sections with the Leica RM2155 microtome (Leica, Wetzlar, Germany)and were rehydrated in xylene and a graded alcohol series using the Leica autostainer XL (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Pathology 2021Quote: ... and then paraffin-embedded as block tissues which were subsequently sectioned with 7-μm thickness by a microtome (RM2235, Leica Microsystems Inc.). After that ...
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bioRxiv - Neuroscience 2023Quote: ... Lung samples were embedded in paraffin wax prior to sectioning at 7 µm using a Hyrax M25 Rotary Microtome (Leica Biosystem, Germany). Sections were stained with hematoxylin and ethanol-based eosin and assessed by a veterinary pathologist who was blinded to the study designs ...
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bioRxiv - Bioengineering 2023Quote: ... Lateral and longitudinal cross-sections of the samples were imaged after 7 days of culture in EGM-2 BulletKit medium using a Thunder imaging system (Leica Microsystems, Germany).
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Neuroscience 2023Quote: ... After washing in PBS (Phosphate Buffered Saline) specimens have been embedded in 7% agarose and cut into 70-100 μm sections using a vibratome (Leica VT1000S; 81). Sections have been washed three times 10 min in 0.3% PBT (PBS with 0.3% TritonX-100) ...
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bioRxiv - Plant Biology 2023Quote: ... Disease phenotypes on the coleoptiles were assessed at 7 days post inoculation by imaging lesions on a Leica M205 FA Stereomicroscope (Leica Microsystems, UK). Each experimental replicate contained 5 biological samples for each treatment (3 mock-inoculated ...
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bioRxiv - Cell Biology 2020Quote: ... Serial thin sections (100 nm) were cut using a Leica UC 6 ultramicrotome (Leica, Wetzlar, Germany) from the surface of the block until approximately 4-5 microns in to ensure complete capture of the cell volumes ...
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bioRxiv - Developmental Biology 2021Quote: ... The dynamics of embryo spreading were examined for 6 hours under a Leica M165C (Leica, German) microscope coupled to a Leica DFC420 (5.0 MP ...
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bioRxiv - Biochemistry 2023Quote: ... Samples embedded in Epon EmBed812 resin were ultrasectioned (80nm) with an ultramicrotome (Leica EM UC 6) and placed on copper mesh 300 previously coated with uranyl acetate and lead citrate ...
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bioRxiv - Plant Biology 2022Quote: ... Stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Cross-sections were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were then sliced in 8-μm sections with a RM2145 Leica microtome (Leica Microsystems GmbH, Wetzlar, Germany) and then hybridized as described previously72 ...
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bioRxiv - Microbiology 2023Quote: ... Images of infected cultures were acquired with a Leica Stellaris 8 Confocal Microscope (40× magnification, 1.3 oil) and analyzed using the LasX software (Leica).
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bioRxiv - Bioengineering 2022Quote: ... and placed on a microscope slide to image with a confocal microscope (Leica Stellaris 5 confocal with DMI 8). The settings for fluorescent imaging were calibrated using a positive control ...
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bioRxiv - Developmental Biology 2023Quote: ... outside of the necrotic region) (Figure 8-figure supplement 1E-F) from 2 sections with the Application Suite Core v2.4 software (Leica).
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bioRxiv - Biochemistry 2023Quote: ... Femurs were cut at a cryosection thickness of 8 μm using a CM3050 S cryostat (Leica Biosystems, Wetzlar, Germany). Transparent Cryofilm 3C 16UF (SECTION-LAB ...
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bioRxiv - Genetics 2024Quote: ... Time-lapse recording was performed on a Leica DMi 8 equipped with a Leica DFC9000 sCMOS camera and a 1.25x HC PL Fluotar objective (Leica). Specially ...
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bioRxiv - Immunology 2024Quote: Whole pancreas explants from Il33Cit/+ mice were imaged on an inverted laser-scanning confocal microscope (Stellaris 8, Leica Microsystems) with an attached pulsed Coherent Chameleon Ultra II laser ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Plant Biology 2020Quote: ... The hairy roots were embedded in 7% agarose before being transversely sectioned at a thickness of 50 μm using a vibratome (Leica VT1000S, Leica, Germany). The safranin O-green staining method was used to detect the deposition and composition of lignin(Bouvier et al. ...
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bioRxiv - Bioengineering 2022Quote: ... Dehydrated samples were embedded in paraffin and then cut in 7 μm thick sections using a rotary microtome (HistoCore AUTOCUT, Leica Biosystems, Nussloch, Germany). Sections were deparaffinised ...
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bioRxiv - Genetics 2021Quote: ... three sets of N ≥ 8 per genotype were pinched and scored for open wounds under a fluorescent stereo microscope (Leica MZ16FA with Planapo 1.6x objective and appropriate filters) ...
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bioRxiv - Immunology 2022Quote: ... target cells were labeled with CMTMR at 10 µM and 1:1000 Hoechst 33342 for 30 min at 37°C, and live imaged in ibidi 8 well chamber slides (ibiTreat, #80826) by confocal microscopy (SP8, Leica) with z-stacks (0.22 µm step ...
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bioRxiv - Immunology 2019Quote: ... mIRAK1mCherry Irak1−/− iBMDMs seeded on 8-chamber coverglass slides were infected with GFP expressing Salmonella Typhimurium and imaged at 63X on SP8 (Leica). mIRAK1mCherry Irak1−/− iBMDMs seeded on Bioptechs interchangeable coverglass dish were treated with TLR ligands and imaged at 60X on optical components built around an Olympus IX71 fluorescence ...
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bioRxiv - Cell Biology 2019Quote: Super-resolution imaging was performed on a Leica TCS SP8 X-White Light Laser confocal microscope equipped with an inverted microscope (DMI 8, Leica), a 100× objective (HC PL APO CS2 100×1.4 oil) ...
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bioRxiv - Neuroscience 2020Quote: ... or superior frontal gyrus were sectioned at 8 μm and collected onto laser capture microdissection (LCM) compatible PET slides (Leica). Sections were stained with cresyl violet to localize regions of interest for LCM (54 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TauRDP301S-CFP/YFP and HEK293 TauRDP301S-CFP cells treated with Tau condensates were fixed with 4% PFA in PBS prior to confocal FLIM on a Stellaris 8 FALCON (Leica) equipped with a 100x objective ...
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bioRxiv - Plant Biology 2021Quote: ... The stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Some of the cross-sections were used for FT-IR analysis and the others were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Neuroscience 2021Quote: ... and continuously perfused with HEPES-based Ringer’s solution via am VC 8 perfusion system (Warner Instruments, Cat. No.640186) directly triggered by the recording software (Leica). Images were acquired at 9.8 Hz at a resolution of 512 x 300 pixels using a 488 nm laser in the resonant ...
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bioRxiv - Biophysics 2021Quote: Lifetime-tuning STED (τ-STED) measurements were performed using a Leica STELLARIS 8 Falcon τ-STED (Leica Microsystems, Mannheim, Germany) inverted confocal/STED microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Images were acquired every 15 min for 16 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL APO 63x/1.4 Oil ph3 objective Leica) ...
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired every 15 min at 30°C for 17 h using a Leica DMi-8 epifluorescent microscope fitted with a DFC365 FX camera (Leica) and Plan-Apochromat 63x oil objective (HC PL 614 APO 63x/1.4 Oil ph3 objective Leica) ...
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bioRxiv - Immunology 2022Quote: ... Samples were then coiled into “Swiss rolls” and embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and sectioned at 8 μm on a CM1950 cryostat (Leica). Immunofluorescent staining was performed in PBS with 1% BSA at room temperature (RT ...
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bioRxiv - Microbiology 2022Quote: Image processing was performed using the software Fiji59 after exporting the images of Cy3 fluorochrome and chlorophyll to 8-bit greyscale TIFF images using the software LAS X (Leica). Each greyscale image was converted to a binary image by a thresholding function using the algorithm “Max entropy”76 ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana was mounted with water on microscope slides and visualized by using a high-resolution laser confocal microscope (STELLARIS 8 FALCON, Leica). The signals of the mNeongreeen were detected at 488 nanometer (nm ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3-8 randomly chosen fields per view per Matrigel assay were acquired using confocal Z-stack imaging (TCS-SP8; Leica). Attached pericytes were counted by using the LASX Software.
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bioRxiv - Cell Biology 2023Quote: Z-stack images were obtained using the Leica STELLARIS 8 FALCON Confocal Microscope and image analysis was performed with Aivia 11 (Leica). To quantify LDs ...
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bioRxiv - Plant Biology 2023Quote: ... These were dehydrated with successive gradients of ethanol (30-100%) and permeated with paraplast to cut 8-12μm sections (using Leica Microtome). The human fat biopsy tissue and potato tubers were processed similarly ...
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bioRxiv - Bioengineering 2023Quote: The third method to assess the micropattern feature dimensions was confocal microscopy (STELLARIS 8, Leica; Fig. 1c, Supp. Fig. 1c)) ...
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bioRxiv - Cell Biology 2023Quote: ... High-resolution images were captured by a confocal Leica STELLARIS 8 microscope equipped with HC PL APO 63×/1.40 OIL objective using type F immersion oil (Leica, 11513859), DAPI was excited by 405 nm laser ...
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bioRxiv - Cell Biology 2023Quote: ... and 561 nm (ER-staining and Alex Fluor 568) and visualized with the Leica Stellaris 8 FALCON Confocal Microscope (Leica) using the objective ...
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bioRxiv - Bioengineering 2023Quote: Diffusivity was assessed in the extravascular space (adjacent but outside of vessels observable in images) for each flow condition using FRAP measurements on a Stellaris 8 confocal microscope (Leica). Devices were perfused with 70 kDa FITC labelled dextran and incubated at 37 °C for 12 hours to allow total diffusion throughout the gel matrix (extravascular space) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by determining the fluorescence lifetime of GFP in different cells cultured in a humidified chamber with 5% CO2 at 37 °C using a STELLARIS 8 FALCON (Leica) systems equipped with HyD X and HyD SMD detectors and an HC PL APO CS2 63x/1.40 OIL objective (Leica) ...
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bioRxiv - Systems Biology 2023Quote: ... The bright-field and immunofluorescent images were acquired using a Pannoramic MIDI system (3DHISTECH) and confocal microscopy (STELLARIS 8 WLL, Leica).
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bioRxiv - Neuroscience 2023Quote: ... Digital images of DAB-stained sections were acquired using a whole slide scanner (3D-Histech Pannoramic 250 Flash III, Sysmex Deutschland GmbH, Norderstedt, Germany or Aperio Versa 8, Leica Biosystems Nussloch GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... The fresh Phenol Red-free DMEM media with 10% FBS was added prior to confocal microscopic experiment (Leica Stellaris 8). A 405 nm laser excitation was used to image BFP and a 488 nm laser excitation was used to image EGFP.