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Citations for Leica :
451 - 500 of 549 citations for 7 Azaspiro 3.4 octane 6 8 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The embedded tissues were then sectioned into 6-μm slices at −20 °C using a CM1950 Cryostat (Leica), followed by fixing in 4% paraformaldehyde for 10 min ...
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bioRxiv - Physiology 2023Quote: ... The embedded tissues were then sectioned into 6- μm slices at −20 °C using a CM1950 Cryostat (Leica), followed by fixing in 4% paraformaldehyde for 10 min ...
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bioRxiv - Genomics 2024Quote: ... The resin blocks were sectioned at 6 μm thick by using a Leica SP1600 microtome (Leica Microsystems, Germany). The fluorescence signals of calcein (green ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Neuroscience 2023Quote: ... DCX+/CLR- and DCX-/CLR+ cells) were counted using Image J (Fiji 1.46) on 7 physical disectors obtained with confocal microscopy (Leica TCS SP5, oil immersion 63× objective) in each animal ...
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bioRxiv - Molecular Biology 2019Quote: ... sections from OCT-preserved samples were cut at a thickness of 8 μm using a cryotome (Leica Biosystems; Buffalo Grove, IL, USA) and were adhered to positively-charged histology slides ...
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bioRxiv - Neuroscience 2020Quote: ... of 2 μm between each plane (8 μm in total) were recorded and merged via the maximum projection tool in the LASX-software (Leica, Mannheim, Germany). The fluorescence intensity curves were measured along the cell nucleus within a region of interest (ROI ...
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bioRxiv - Physiology 2021Quote: Frozen heart sections embedded in OCT compound (Tissue-Tek®; Sakura, UAE) were cut into 8 µm sections with a cryostat (Leica, Germany), permeabilized ...
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bioRxiv - Microbiology 2022Quote: ... Single-cell resolution images of fixed samples were acquired using a DMI8 Leica SP-8 point scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 63x water immersion objective (Leica ...
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bioRxiv - Physiology 2021Quote: Liver tissue samples embedded in Tissue Tek (Weckert, Kitzingen, Germany) were cryosectioned (8 μm thick) using a Leica CM3050 S (Leica, Bensheim, Germany) cryostat microtome ...
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bioRxiv - Biochemistry 2021Quote: ... Femurs were cryosectioned at 8 μm thickness using a DB80 HS microtome blade in a CM3050 S cryostat (Leica Biosystems, Wetzlar, Germany) set to −25 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... (Sakura Finetek; Alphen an den Rijn, Netherlands) for cryo-sectioning at 8 µm sections using a CM1850 Cryostat (Leica Biosystems; Wetzlar, Germany).
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bioRxiv - Neuroscience 2022Quote: ... and a Leica STELLARIS 8 confocal microscope with a 20x HC PL APO NA 0.75 multi-immersion objective (Leica Microsystems, Wetzlar, Germany). Raw images were projected with Fiji (Schindelin et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the specimens were embedded in Technovit 8100 (Kulzer, Germany)100 and cut into 8 µm sagittal sections using a Leica HistoCore AUTOCUT R microtome (Leica, Wetzlar, Germany) equipped with glass knives ...
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bioRxiv - Plant Biology 2020Quote: ... The division and elongation of AR founder cells were observed with a stereoscopic microscope (Leica MZ 6; Wetzlar, Germany) and ImageJ (v ...
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bioRxiv - Cell Biology 2020Quote: ... Resin was polymerised at 60°C overnight and 60 nm ultrathin sections were cut on an Ultracut 6 (Leica) ultramicrotome and imaged as described above.
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bioRxiv - Neuroscience 2019Quote: ... brains from 6-month-old 5xFAD and WT animals were coronally sectioned through the hippocampus on a vibratome (Leica; settings ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was embedded in 6% agarose and coronal sections (40 μm) were cut using a vibratome (Leica VT1200). A total of 30 sections starting from Bregma −1.22 mm (Paxinos and Franklin(2004) ...
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bioRxiv - Developmental Biology 2021Quote: Live imaging of Rx3::H2B-GFP-derived organoids (Video 5 and 6) was performed with Sp8 confocal microscope (Leica). Organoids were imaged from day 1 to day 2 at room temperature directly in low binding 96-well plate (Nunclon Sphera U-Shaped Bottom Microplate ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 6% agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000s; Wetzlar, Germany). Slices were stored in PBS and 0.05% sodium azide at 4 °C before staining.
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bioRxiv - Plant Biology 2022Quote: ... images were taken using 6-day old young seedling using Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems).
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bioRxiv - Biochemistry 2022Quote: ... Images were acquired as z sections at 0.27 μm (using Slidebook Software 6 from Intelligent Imaging Innovations or using LCS 3D software from Leica). Images were converted into maximal intensity projections ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 100µm were performed at medium speed (around 5) and amplitude (approx. 6) using a vibratome Leica VT1000S (Leica). Sections were placed on a glass slide for immunostaining.
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bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were sectioned at 50µm (1 in 6 series) in the coronal plane with a vibratome (model VT1000, Leica). Sections were stored at -20°C in a cryoprotectant solution comprised of 60% glycerol and 0.01% sodium azide in 0.1M PBS.
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bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
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bioRxiv - Biophysics 2019Quote: ... were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems, Wetzlar, Germany), before 5 μl aliquots of the liposome/protein solution were applied to the grids ...
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bioRxiv - Neuroscience 2021Quote: ... Red light illumination was provided by 8 individually addressable LEDs (WS2812) attached to the objective (Leica Z6 APO coupled with a Leica PlanApo 2.0X 10447178) by a custom 3D-printed conical holder ...
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bioRxiv - Plant Biology 2021Quote: ... Inflorescence stem subjected to GUS staining were then embedded in 8% agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Plant Biology 2021Quote: ... GFP and YFP signals were imaged on a Leica SP2 or Leica Stellaris 8 Laser Scanning Confocal Microscope (Leica Microsystems, Buffalo Grove, IL) at 48hpi ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of paraffin sections with 8 µm thickness were continuously cut using a Leica RM2255 microtome (Leica Biosystems Inc. Buffalo Grove, IL) and mounted on Superfrost Plus microscope slides (cat# 12-550-15 ...
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bioRxiv - Plant Biology 2022Quote: ... Inflorescence stems subjected to GUS staining were then embedded in 8% (w/v) agarose and sectioned with a Leica VT100S vibratome (Leica, https://www.leica-microsystems.com/). Sections were counterstained for lignin by phloroglucinol staining (Pradhan Mitra and Loqué ...
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bioRxiv - Neuroscience 2024Quote: ... High-resolution microphotographs (1024×1024 pixel resolution and 400Hz acquisition speed) were taken at 20X magnification using an inverted Stellaris 8 confocal microscope (Leica Microsystems, Wetzlar, Germany). Same laser gain ...
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bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
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bioRxiv - Neuroscience 2020Quote: 200 μm-thick hippocampal sections were isolated from 16 C57BL/6 mice of P22-28 using a vibratome (Leica, VT1200S), in the presence of ice-cold oxygenated a-CSF (125mM NaCl ...
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bioRxiv - Immunology 2022Quote: Tissue samples were fixed for a minimum of 7 days in 10% neutralized buffered formalin before being removed from BSL-4 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were fitted onto sample stubs using carbon adhesive tabs and sputter coated with 6 nm Iridium (Leica SCD500). Samples were imaged at 2 KV ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Microbiology 2021Quote: Tissue samples were fixed for a minimum of 24 h in 4% PFA before being removed from BSL-3 and subsequently processed in a Tissue-Tek VIP-6 automated vacuum infiltration processor (Sakura Finetek) and embedded in paraffin using a HistoCore Arcadia paraffin embedding machine (Leica). 5 μm tissue sections were generated using an RM2255 rotary microtome (Leica ...
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bioRxiv - Immunology 2020Quote: ... Paraffin blocks were sectioned from posterior to anterior at 6-μm thickness using a rotary microtome (Leica RM2255, Wetzlar, Germany). Sections were stained using Safranin-O/Fast Green at 90-μm intervals to assess cartilage morphology in the medial tibial plateau ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Cell Biology 2023Quote: ... Transplanted embryos were mounted in 0.2% agarose in embryo medium and imaged between shield stage and 85% epiboly (6 - 9 hpf) under an inverted TCS SP8 confocal microscope (Leica) equipped with environmental chamber (Life Imaging Services ...
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bioRxiv - Cell Biology 2023Quote: ... and an Andor DU-888 X-11056 camera (1,024 x 1,024 pixels). Other live-cell images (Fig. 6) were acquired on confocal DIVE-FALCON laser scanning confocal microscope (Leica Microsystems) controlled by the Leica LAS X Life Science software using the HCX PL APO CS 63.0×/1.30 NA glycerol immersion objective and 488 nm and 633 nm lasers ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...
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bioRxiv - Neuroscience 2021Quote: ... 8–10 week old mice received a single impact onto the skull at bregma using an ImpactOne stereotaxic impactor (Leica Biosystems, Buffalo Grove, IL) with a 3-mm-diameter tip (velocity set at 4.0 m/s ...
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bioRxiv - Cell Biology 2023Quote: ... Cryosections (8 μm) were cut on a cryostat HM 505E (Microm, Walldorf, Germany) equipped with a CryoJane Tape-Transfer system (Leica Inc., Buffalo Grove, IL). For labeling ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were sectioned into 6 µm thick cross sections with a Leica CM3050 S Research Cryostat (Leica Microsystems, Wetzlar, Germany), mounted on microscope slides and fixed with 1 % PFA in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).