Labshake search
Citations for Leica :
351 - 400 of 2029 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
-
bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...
-
bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were extracted from the skull and stored in 4% formaldehyde before being cut in sagittal sections (50 μm) using a vibratome (Leica VT1000) and stained with cresyl violet ...
-
bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
-
bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
-
bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
-
bioRxiv - Bioengineering 2021Quote: ... according to standard histological procedures and 4 μm thick sections of the paraffin blocks were cut on the microtome (Leica, HM325). The paraffin sections were stained with Haematoxylin-Eosin (HE ...
-
bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
-
bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
-
bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
-
bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were perfused with 4% PFA in PBS and brains were removed and sectioned coronally or sagittally on vibratome (Leica VT1000) at 50-75 μm and mounted on slides with ProLong(tm ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde in PBS and imaged using laser scanning confocal microscopy (LEICA DMi8, Leica Microsystems, Wetzlar, Germany).
-
bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed with 4% PFA in PB overnight and 50-µm coronal sections of the brainstem were obtained on a vibratome (Leica VT1200S). Expression of EGFP at the calyx of Held terminals was visualized using an epifluorescence inverted microscope (CKX41 ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed overnight at 4°C and cut into 30 μm-thick free-floating coronal sections with a vibrating microtome (Leica, VT1000). All the following steps were done with gentle shaking ...
-
bioRxiv - Microbiology 2024Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using an ACE600 cryo-sputter coater (Leica Microsystems GmbH) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy) ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The other hemibrain was drop-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and sectioned (30 μm) with a sliding microtome (Leica SM2000R). All mouse experiments were performed in accordance with European Union Council Directive 86/609/EEC ...
-
bioRxiv - Microbiology 2023Quote: ... samples were stored light-protected in the same buffer at 4°C until imaging with a Leica TCS SP5 II laser scanning confocal microscope (Leica Microsystems) using sequential scanning mode.
-
bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
-
bioRxiv - Neuroscience 2023Quote: ... containing the dorsal hippocampus were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... sagittal sections at 5° angle tilted to the midline with 300 μm thickness were obtained inside the same slicing solution at 4°C using Leica VT1200S vibrotome (Leica Biosystems). Slices were immediately transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Neuroscience 2023Quote: ... The L4 segments were fixed overnight at 4°C and cut into 60 μm thick transverse sections with a vibrating blade microtome (Leica VT1200). The sections were incubated for 3 days in rabbit anti-GFP (see Table S2) ...
-
bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
-
bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
-
bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
-
bioRxiv - Cell Biology 2023Quote: ... confocal microscope in 3 Z-stacks at 20x (1024x1024 resolution) and further imaged at 63x with immersion oil in 4 Z-stacks to highlight regions of interest (Leica Sp8x).
-
bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
-
bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
-
bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
-
bioRxiv - Neuroscience 2024Quote: ... Mouse brains were post-fixed in 4% PFA overnight and sectioned coronally at 50 µm thickness (approximately 240 sections per brain) on a vibratome (Leica VT1200S). Brain sections containing regions of interest were permeabilized in 1% Triton X-100 in TBS and blocked in 5% normal goat serum ...
-
bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA at 4 °C and then rinsed in PBS before being sectioned at a thickness of 60 µm using a vibratome (Leica Biosystems). These sections were then mounted onto Superfrost Plus slides (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... and LHA (for recordings) were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000, Ted Pella, or VT1000P, Leica). Sections were collected into cryoprotectant (25% glycerol ...
-
bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
-
bioRxiv - Immunology 2020Quote: ... 2-photon laser scanning microscopy was performed with a LEICA SP5 inverted 5-channel confocal microscope (Leica Microsystems) equipped with a 25X water-immersion objective ...
-
bioRxiv - Bioengineering 2022Quote: ... 3′-diaminobenzidine (Leica).
-
bioRxiv - Plant Biology 2020Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
-
bioRxiv - Plant Biology 2021Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
-
bioRxiv - Plant Biology 2024Quote: ... and Antirrhinum majus stained with SR2200 and TO-PRO-3 iodide or DAPI was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
-
bioRxiv - Plant Biology 2023Quote: Confocal laser scanning microscopy of ovules stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
-
bioRxiv - Plant Biology 2024Quote: ... and embryos stained with SR2200 and TO-PRO-3 iodide was performed on an upright Leica TCS SP8 X WLL2 HyVolution 2 (Leica Microsystems) equipped with GaAsP (HyD ...
-
bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
-
bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
-
bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
-
bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).