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Citations for Leica :
501 - 550 of 2029 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
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bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
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bioRxiv - Neuroscience 2024Quote: ... were cut at 1 °C–2 °C using a vibratome (Leica VT1200S, Wetzlar, Germany). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were merged in 10% and 30% sucrose-PBS solutions at RT (2 h each solution) followed by 1:1 solution 30% sucrose:tissue-freezing medium (Leica 14020108926). Afterwards ...
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bioRxiv - Neuroscience 2023Quote: ... Stellaris 5 (Leica), or LSM 900 (Zeiss ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 spectral detectors (HyD-RLD, Leica) and a 37°C preheated 40X objective (NA 1.10 ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...
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bioRxiv - Cancer Biology 2024Quote: ... for transferrin receptor 1 (TfR1) and using Bond Epitope Retrieval Solution 2 (Leica Biosystems AR9640) for Ki67 and CD31 ...
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bioRxiv - Biochemistry 2022Quote: ... Pelco Easiglow) and vitrified (3 μL sample, 3 s blot time) using an automatic plunge freezer (Leica). EndoX-TSEN•CLP1 was frozen on R1.2/1.3 Cu 300 mesh grids (Quantifoil ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Cell Biology 2021Quote: ... Micro 3 - a DMi8 microscope (Leica Microsystems) with a high-contrast Plan Apochromat 63×/1.30 Glyc CORR CS objective (Leica Microsystems) ...
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bioRxiv - Synthetic Biology 2022Quote: ... #1 prior to plunging into liquid ethane (−182°C) in a Leica EM GP 2 (Leica) vitrification robot (automated blot pressure ...
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bioRxiv - Plant Biology 2021Quote: ... The roots were washed with 1/2 MS media and imaged by fluorescence microscopy (Leica, M205FA). The quantitative measurement of ROS staining intensity was performed as average pixel intensities using ImageJ software [28].
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bioRxiv - Neuroscience 2022Quote: ... at - 45°C for 2 days and UV-polymerized for 1 day in EM AFS2 (Leica). The aBA-containing polymerized blocks were further sliced into 100 nm thickness by an ultra-microtome (Leica) ...
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bioRxiv - Neuroscience 2024Quote: ... at −45 °C for 2 days and UV polymerization for 1 day with EM AFS2 (Leica). UV-polymerized blocks containing the NAcSh were sliced by an ultramicrotome (Leica) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed for 1–3 days and sectioned coronally (50–100 μm in thickness) using a vibratome (Leica; VT1000S). Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes) ...
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bioRxiv - Plant Biology 2022Quote: ... seedlings were mounted on a glass slide in 1/2 MS medium and imaged on SP8 (Leica). YFP was excited by the 514-nm line of the argon laser and detected between 525 and 580 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... GV-stage oocytes were injected with 10 pl of a solution containing 0.1 mM Emi2-3’UTR-MO or Emi2-3’UTR 5mm-MO using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium ...
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bioRxiv - Microbiology 2020Quote: ... coated with 3 nm gold/palladium (Leica CPD300) and examined with a JEOL JSM-7400F scanning electron microscope (3 kV-LEI detector).
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bioRxiv - Neuroscience 2023Quote: ... stained with 3% lead citrate (Leica UltroStain II) for 3 m ...
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bioRxiv - Immunology 2024Quote: ... the tip of the 3-mm impactor (Leica) piston was angled and kept perpendicular to the exposed cortical surface ...
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bioRxiv - Developmental Biology 2022Quote: ... and Stellaris 5 (Leica Microsystems) confocal microscopes.
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Bioengineering 2024Quote: ... was added to the confocal dish with a 3 mm x 3 mm hole and observed under a confocal microscope (STEDYCON, Leica, Germany).
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Plant Biology 2020Quote: ... Sections (3-4μm) cut by rotary microtome (Leica RM2265) were stained with 0.01 % aqueous toluidine blue ...
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Neuroscience 2021Quote: ... and 3% lead citrate (Leica Ultrostain II, Leica Microsystems). Images were collected semiautomatically using custom software guiding an FEI Magellan XHR 400L54.
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µm sections were cut on a microtome (Leica), rehydrated through an alcohol series according to standard laboratory procedures and then stained with a PAS staining kit (TCS Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µm sections were obtained with a microtome (Leica RM2255 Fully Automated Rotary Microtome) ...
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bioRxiv - Microbiology 2024Quote: ... and IBA1 using the Bond- RX/3 autostainer (Leica) with the BPRD kit (DS9800 ...
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bioRxiv - Genomics 2020Quote: ... Microtome sections (5 μm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Cell Biology 2024Quote: ... Microtome sections (5 µm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Physiology 2021Quote: Immunohistochemical staining in pancreas and fat sections was carried out on 1-2 μm section cut from paraffin blocks in an automated immunostainer from Leica biosystems (BOND RX ...
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bioRxiv - Immunology 2021Quote: ... Sections were serially imaged over the course of 1-2 hours and compiled using LAS X software (Leica Microsystems, USA). Image analysis was performed using Imaris software (Bitplane ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...