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Citations for Leica :
351 - 400 of 1153 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and the contusion was induced using a 3 mm convex tip attached to an electromagnetic impactor (Leica). The contusion depth was set to 0.95 mm from dura with a velocity of 4.0 m/s sustained for 300 ms ...
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bioRxiv - Immunology 2024Quote: ... Nine TLS+ Glioma FFPE sections (3 µm) were generated using the Leica SM 2000R microtome (Leica Microsystems), and incubated at 37 C overnight ...
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bioRxiv - Physiology 2024Quote: ... Blocks were cut into thick (10 μm) serial (3-4) sections and placed onto PEN-slides (Leica) along with thin (4 μm ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were then transferred to a 30 % sucrose solution and sliced coronally using a cryostat (Leica CM1850). Slices were mounted with DAPI-mounting medium (Vector Laboratories ...
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bioRxiv - Genomics 2020Quote: ... in 4μl of Ringer’s solution (made with DEPC water) + 0.01% Tween 20 and dissected under a stereomicroscope (Leica M80) with an X-ACTO knife (Amazon cat# B01MUWTH89) ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... mounted in Clearsee Alpha solution and observed using a confocal TCS SP5 or TCS SP8 confocal microscope (Leica). Auramine O was excited at 488 nm and the emission was collected at 500-570 nm.
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bioRxiv - Neuroscience 2020Quote: ... 300 μm-thick transverse slices were prepared in the cutting solution using a vibratome (Leica VT1200, Nussloch, Germany), and incubated at 34 °C for 30 min in the same solution ...
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bioRxiv - Cell Biology 2022Quote: ... The staining solution was removed and the samples were then imaged under a fluorescence microscope (DMi8, Leica, Germany) with 494 nm (green ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions: After antigen retrieval in a pH9 EDTA buffer based epitope retrieval solution (Leica), sections were incubated with monoclonal antibodies against Ki67 (rabbit anti-Ki67 ...
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bioRxiv - Physiology 2023Quote: ... Coronal spinal cord slices (400 µm) were cut in the cutting solution using a vibratome (VT-1200S, Leica) and transferred to artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were loaded into Leica Bond RX and pretreated with EDTA-based epitope retrieval ER2 solution (Leica, AR9640) for 20 mins at 95°C ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Bioengineering 2022Quote: ... Cell pellets were reconstituted in rat serum and stained with Leishman solution on frosted glass slides (Leica Biosystems). Using a light microscope (BX2 ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Molecular Biology 2023Quote: ... All solutions and high-pressure frozen sapphire discs were loaded to the automated freeze substitution system AFS2 (Leica). A freeze substitution program was performed according to the following table:
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bioRxiv - Microbiology 2024Quote: ... with a tissue pretreatment of 30 minutes at 100°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) and 1:750 dilution of OPAL 570 (AKOYA ...
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bioRxiv - Microbiology 2024Quote: ... and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems, Wetzlar, Germany). All fixed tissues were then processed in ethanol and xylene in a tissue processor and embedded in paraffin (Leica ASP6025 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
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bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...
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bioRxiv - Microbiology 2021Quote: ... Confocal imaging was performed with a Leica TCS SP8 STED 3× laser scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 100×/1.4 Oil HC PL APO CS2 STED White objective ...
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bioRxiv - Cell Biology 2023Quote: ... The cryosection ribbon (∼3 mm long) was then attached to the grid by operating the Crion (Leica Microsystems) in “charge” mode for ∼30 seconds (Pierson et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were rinsed in tap water and dipped 3-5 times in 0.5% Acid Alcohol (Leica Biosystems 3803651), followed by rinsing in tap water and bluing with Scott’s Tap Water (Electron Microscopy Sciences 2607007 ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in blocks of 3% agarose and sectioned in 100 µm slices on a vibrating blade microtome (Leica) or mounted as whole droplets.
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bioRxiv - Neuroscience 2022Quote: ... then following fixation they were imaged with an inverted confocal microscope with the laser power increasing for 0.2 to 3% with 0.2% intervals (Extended Data Fig. 1a, b, SP8 TCS with HyD detectors, Leica). Laser power was linearly controlled by ATOF ...
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bioRxiv - Plant Biology 2022Quote: ... leaves were harvested at 3 dpi for aniline blue/calcofluor staining and analysis by epi-fluorescence microscopy (Leica DM6B ...
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bioRxiv - Plant Biology 2022Quote: ... GUS-stained leaves were embedded in 3% agarose and sectioned with a vibrating blade microtome (Leica VT1000 S). Images were taken using ZEISS Axio Observer.
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bioRxiv - Neuroscience 2023Quote: ... the ISH-mounted specimen was first loaded into a pre-tilted specimen block (Leica; Figure 3 J&K). The block was then placed into a Leica VCT-100 cryo-transfer system under liquid nitrogen to facilitate transfer into the pre-cooled cryo-FIB/SEM cryo-substage.
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Adult choline supplementation in a Down syndrome model reduces co-morbidities and improves cognitionbioRxiv - Neuroscience 2024Quote: Whole brains were embedded in 3% agarose in 1x PBS and sectioned coronally using a vibratome (Leica VT1000S) at a thickness of 50μm ...
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bioRxiv - Cell Biology 2024Quote: ... After neutralization of endogenous peroxidase with 3% H2O2 and Fc blocking with 0.4% casein in PBS (Leica Biosystems), the sections were incubated with the following primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... heat-induced epitope retrieval (HIER) was performed using a ready-to-use citrate-based solution (pH 6.0; Leica Biosystems) at 100 °C for 20 min ...
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bioRxiv - Genomics 2020Quote: ... in 4μl of M9 (made with DEPC water) + 0.01% Tween 20 solution and dissected under a stereomicroscope (Leica M80) with an X-ACTO knife (Amazon cat# B01MUWTH89).
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bioRxiv - Neuroscience 2020Quote: ... Coronal sections of dLGN (320 μm) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were carefully extracted and transferred to a chamber filled with the same solution on a vibratome (VT1200; Leica). Brains were sliced at 250 μm (coronally for recording of SNr and PF neurons ...
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bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
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bioRxiv - Cell Biology 2020Quote: 10 μL of the nuclei solution was pipetted on a hemocytometer (Neubauer) for visualization under the (Leica DM IL inverted) microscope ...
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bioRxiv - Neuroscience 2022Quote: ... the apical turn cochlear organs from 1-2 months old Pclowt/wt and Pclogt/gt rats were dissected in ice-cold HEPES Hank’s solution and mounted onto type A specimen carriers (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
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bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue blocks were cut coronally with a thickness of 300 μm in this solution using a vibratome (Leica VT1200S). Slices were collected and then incubated in aerated ACSF at 35 °C ...
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bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 5μm sections were stained with hematoxylin (of Mayer) and 0.2% eosin (ethanolic solution; both Medite) in an automated staining machine (Leica). Ileal and colonic sections were blindly assessed for inflammation (tissue pathology index ...