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Citations for Leica :
451 - 500 of 1153 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and subjected to automated baking and deparaffinization followed by heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) at 100 °C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... Brains was postfixed overnight at 4 °C in a 4 % formaldehyde solution and then embedded in 5 % agarose gel for sectioning (50-µm-thick coronal sections) with a vibratome (VT1000S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... brains were rapidly removed and coronal or horizontal slices were cut in ice-cold cutting solution on a Leica VT1000 S microtome (Leica Microsystems Inc ...
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bioRxiv - Biophysics 2024Quote: A small portion of the isolated chromosomes was stained with DAPI and imaged in solution on a glass bottom dish with an inverted fluorescence microscope (Leica DMi8 or Leica DMI6000B ...
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bioRxiv - Biophysics 2024Quote: A small portion of the isolated chromosomes was stained with DAPI and imaged in solution on a glass bottom dish with an inverted fluorescence microscope (Leica DMi8 or Leica DMI6000B ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was rapidly dissected and cut into 250 µm thick coronal slices in the same solution on a vibratome (VT1200S, Leica). Slices were then transferred to 34°C warm artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Microbiology 2024Quote: ... were subjected to automated heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based retrieval solution (pH 9.0, Leica Biosystems) at 100 °C for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... Horizontal sections (250 μm) containing the VTA were cut in NMDG solution using a vibratome (VT1200, Leica Microsystems, Nussloch, Germany). Slices were then incubated in NMDG solution (32°C ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was promptly placed in ice-cold oxygenated cutting solution and sectioned at 250 μm using a vibratome (VT1200S, Leica). Immediately after the sectioning ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were reconstituted in an antifade solution (0.1% p-phenylenediamine in 70% glycerol) and examined under a confocal laser scanning microscope (Leica, Stellaris) with a 63x objective ...
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bioRxiv - Cancer Biology 2021Quote: ... Each wash was performed for 3 min at room temperature with repetitive dipping using a linear stainer (Leica, #ST4020). Slides were transferred for heat-induced epitope retrieval to a PT module (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: Fertilized ovaries from B73 plants were collected 3 days after pollination (DAP) and sliced using a Vibratom (Leica VT1000E) to create 200 to 225 μm sections ...
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bioRxiv - Cancer Biology 2020Quote: ... two sections of formalin-fixed paraffin-embedded tissues of FMC with 3 µm thickness (Microtome Leica RM2135, Newcastle, UK) were prepared ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose and sliced (100 μm thickness) using a vibrating blade microtome (Leica VT1000S, Leica Biosystems, Germany). The slices were then blocked for 1-2 hrs in 10% sheep serum / 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Neuroscience 2021Quote: ... Three (3) brains per group were later sectioned with a cryostat (Cryocut 1800 Cryostat, Leica Biosystems, Vista, CA, USA) at 25 μm thickness in the coronal plane ...
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bioRxiv - Immunology 2020Quote: ... Lung tissues were embedded in 3% LMA and 100μm-thick sections were cut at the vibratome (Leica VT 1200S). Sections were placed on microscope slides ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea conidia and germlings exposed to 3 μM NCR044.1 was also imaged using fluorescence confocal microscopy (Leica SP8-X)39 with an excitation and emission wavelength of 480 and 510-566 nm ...
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bioRxiv - Genomics 2021Quote: ... Formalin-fixed agarose/paraffin-embedded sections (3-5µm) were manually cut from blocks with a microtome (Leica RM 2145) and transferred to glass slides (Superfrost ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
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bioRxiv - Developmental Biology 2022Quote: ... Blastema tissues (n=3 for neotenic and metamorphic animals) were first embedded in a tissue freezing medium (Leica, 14020108926). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
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bioRxiv - Biophysics 2022Quote: ... 300 mesh grids were coated with a continuous carbon layer of 3 nm in sputter coater EM ACE600 (Leica). The continuous carbon layer was first deposited on mica support of 25 x 25 mm (Cat Nr 71850-11 ...
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bioRxiv - Neuroscience 2023Quote: ... and cortex region (n=3/group) were cut at 5μm thickness in a rotary microtome (Leica RM2125RT, Wetzlar, Germany) for histological analysis (Venkidesh et al. ...
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bioRxiv - Immunology 2024Quote: ... and single-cell quantification was performed in 3–5 fields imaged with the ACS_APO 20x/NA 0.60 objective of a confocal microscope (Leica, SPE). Mean Fluorescence Intensity (MFI ...
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bioRxiv - Molecular Biology 2024Quote: ... Images were captured at 2 min 30 sec intervals for 3 hours and processed using LAS X software (Leica).
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bioRxiv - Cell Biology 2020Quote: ... NKX2-1 (Leica TTF-1-L-CE), Ki67 (Abcam ab16667) ...
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bioRxiv - Physiology 2021Quote: ... Heat-induced antigen retrieval was performed with citrate buffer (pH 6) for 30 minutes (AR9961; Bond TM Epitope Retrieval Solution; Leica Biosystems) in 2-μm-thick sections ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were incubated with one drop of 3,3’ Diaminobenzidine (DAB) chromogen solution (cat.RE7270-K, Novolink MAX DAB, Leica Microsystems, Wetzlar, Germany) for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Neuroscience 2021Quote: ... 300 µm-thick coronal mPFC slices were prepared in carbogenated ice-cold slicing solution using a vibratome (Leica VT 1200 S) and allowed to recover for 20 min at 33 °C in carbogenated high-osmolarity artificial cerebrospinal fluid (high-osmolarity aCSF ...
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bioRxiv - Neuroscience 2021Quote: ... rapidly removed the brain and sectioned it in an ice-cold carbogen-saturated cutting solution using a vibratome (VT1000S, Leica Microsystems). We then incubated coronal slices (220 µm ...
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bioRxiv - Neuroscience 2021Quote: ... Horizontal slices (250 pm) containing the intralaminar thalamic nuclei were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems) and then transferred to protective recovery solution maintained at 32-34°C for 12 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... Horizontal slices (300 μm) containing the thalamus were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems). Slices were transferred to artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
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bioRxiv - Neuroscience 2022Quote: Perfused brains were cryoprotected with 30% sucrose solution (Fisher Schientific, UK) and sliced at 30 μm using a SM2010R freezing microtome (Leica, UK). The slices were stained with DAPI (HelloBio ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal slices (300□μm thick) from the hippocampus were prepared in ice-cold partial sucrose solution using a microtome (Leica VT1200S) and immediately transferred in standard aCSF containing (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... 4.5 mM MgSO4) and 400 µm coronal slices were prepared in ice-cold aCSF solution using a vibrating microtome (VT1200S, Leica, Germany). Slices were transferred to aCSF solution for 30 minutes at 35 °C and current clamp whole-cell recordings were performed using a Multiclamp 700A amplifier (Molecular Devices ...
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bioRxiv - Neuroscience 2022Quote: Perfused brains were cryoprotected with 30% sucrose solution (Fisher Schientific, UK) and sliced at 50 μm using a SM2010R freezing microtome (Leica, UK). The slices were mounted using HardSet Mounting Medium with DAPI (Vector Labs ...
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bioRxiv - Cancer Biology 2022Quote: ... and rehydration procedure were performed in the system and a 20 min treatment of epitope retrieval solution I (Leica Biosystems, AR9961) was applied ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Bioengineering 2021Quote: ... Antigen retrieval was performed by incubating the sections in Leica Bond Enzyme solution (Bond Enzyme Pre-treatment Kit, Leica Biosystems, Germany) for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 80 µl of the M solution and 5 µl were used for fluorescence microscopy observation under the Leica TCS SP8 confocal microscope (Leica, Germany). The data was analyzed using the Leica Application Suite X imaging and analysis software (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was quickly removed into a beaker containing the ice-cold oxygenated NMDG-based solution before obtaining 300 µm parahorizontal slices using a vibratome (VT1200S; Leica Microsystems). Sections were immediately transferred to recover in well oxygenated NMDG-based solution at 35°C for 5 minutes ...