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Citations for Leica :
301 - 340 of 340 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agarose (3% in Water) and 70 µm coronal sections were cut with a VT1000S vibratome (Leica Biosystems). Every second section ...
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bioRxiv - Microbiology 2020Quote: Confocal laser scanning microscopy was performed with a Leica DMI 6000 TCS SP5 equipped with a HC PL APO CS2 40x water immersion objective (NA = 1.1; Leica, Germany; #15506360), using the Leica Application Suite Advanced Fluorescence software (V ...
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bioRxiv - Microbiology 2020Quote: ... were positioned on preheated flat agar plates (1% agar in egg water with 0.017% MS-222) and imaged with Fluorescence Stereo Microscope (Leica M205 FA). The image acquisition settings were as following ...
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bioRxiv - Neuroscience 2022Quote: ... 3D image stacks were acquired with a 63x/0.9NA water immersion objective and a CCD camera (DFC350 FX) in an upright widefield fluorescence microscope (DM LFSA) (all from Leica Microsystems) using a Cy5 filterset (49006 ...
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bioRxiv - Cell Biology 2023Quote: ... 2-photon system using a Leica HC Fluotar L VISIR 25x/0.95 Water immersion objective lens (working distance 2.4 mm) and equipped with a FALCON FLIM module (Leica Microsystems, Mannheim, Germany). For 2-photon excitation ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were imaged using a 40x water immersion dip-in lens mounted on a Leica TCS SP5 confocal microscope (Leica Microsystems). The excitation wavelength was 488 nm for GFP ...
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bioRxiv - Pathology 2023Quote: ... three rinses in tap water) mounted in glycerol and imaged using a Leica DM2000 microscope with Leica DFC7000 T camera (Leica, Germany).
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bioRxiv - Biophysics 2024Quote: Hyperspectral images were acquired using a confocal Leica SP8 FALCON microscope equipped with a 63× 1.2 NA water immersion objective (Leica, Mannheim, Germany). The microscope was coupled to a pulsed Ti:Sapphire laser MaiTai (SpectraPhysics ...
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bioRxiv - Developmental Biology 2024Quote: ... The glass capillary of the water micromanometer was inserted into the midbrain vesicle through the myometrium under a dissecting microscope (Leica MZ7.5). To ensure the visibility of the colored saline ...
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bioRxiv - Neuroscience 2022Quote: ... The excitation beam was focused with a water-dipping objective lens (Nikon CFI75 16× 0.8NA or Leica HC FLUOTAR L 25x 0.95NA). The average power was 100-150 mW at the sample ...
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bioRxiv - Plant Biology 2021Quote: ... EV pellets or EV coated beads were suspended in infiltration buffer were examined using a 40x water immersion or dip-in lens mounted on a Confocal Laser Scanning Microscope equipped with an argon/krypton laser (Leica TCS SP5).
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bioRxiv - Microbiology 2022Quote: ... LSM was carried out using an HC PL APO 40×/1.10 CS2 water immersion objective on an SP8 confocal microscope (Leica Microsystems, Wetzlar, Germany), equipped with an 80 MHz pulsed white light laser (WLL ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed a couple of times with water before they were visualized under the microscope (Leica DM IRB, TV camera). Five randomly fields were chosen to capture images using the ZEISS Efficient Navigation ...
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bioRxiv - Plant Biology 2022Quote: Protoplast images were obtained using a Leica TCS SP8 confocal microscope equipped with a 20x water immersion lens (Leica Microsystems, Mannheim, Germany), with the exception that images from Fig 2A and Fig 4A-A’’ were taken with a Zeiss LSM880 Airyscan using a 63x oil immersion lens ...
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bioRxiv - Plant Biology 2022Quote: ... The abaxial side of the leaf sample was imaged using a 63× objective (Leica, HC PL APO CS2 5 63×/1.20 Water). GFP was excited with a 488 nm WLL laser and collected at 503-530 nm ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Plant Biology 2023Quote: ... Stems were rehydrated first in ethanol 50% and later in water and then embedded in agarose 5% before being transversally cut using a vibratome (Leica vt 1000s) to obtain 120 micron thickness sections stored in water ...
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bioRxiv - Biophysics 2023Quote: ... Imaging was performed with a 63× 1.20 NA water immersion objective lens (Leica 15506346 HC PL APO 63×/1.20 W CORR CS2) at 490nm and 648nm excitation ...
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bioRxiv - Bioengineering 2023Quote: ... We add DI water to the apical chamber of the Transwell® insert and monitor the fluorescence intensity using a fluorescence confocal microscope (Leica, SP8). The fluorescence intensity is quantified and normalized to the value at the initial time point.
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bioRxiv - Synthetic Biology 2024Quote: ... the fixed samples were dehydrated in a graded series of water-ethanol solutions ranging from 30% to 100% ethanol and dried by a critical point dryer (Leica EM CPD030). The top surface of the BC pellicle was coated with platinum under vacuum and observed via a scanning electron microscopy (Hitachi SU8010 ...
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bioRxiv - Cell Biology 2024Quote: Regions of interest in the TMA were image acquired using Leica STELLARIS 8 confocal microscope equipped with a 63 HC PL APO 63x/1.20 water-immersion CS2 objective (Leica Microsystems. Mannheim, Germany). The settings for each image were Pinhole 1 Airy unit ...
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bioRxiv - Developmental Biology 2020Quote: ... Confocal microscopy images of whole-mount mouse embryos and sections were acquired using a Leica TCS SP5 II multiphoton confocal microscope equipped with a 25X water immersion objective (Leica Microsystems, Wetzlar, Germany). For 3D-projection of whole-mount Z-stacks ...
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bioRxiv - Cancer Biology 2021Quote: Frozen resected tissues were freeze mounted to the microtome block using water and sectioned at 10 μM thickness for lipid analyses using a CM 1950 Cryostat (Leica Biosystems, Nussloch, Germany), and using a blade that was free from optimal cutting temperature (OCT ...
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bioRxiv - Plant Biology 2020Quote: ... protein localisation and plasmolysis we used a Leica TCS SP8 confocal microscope equipped with a 20x HCX PL APO water immersion lenses (Leica Microsystems, Mannheim, Germany). GFP was excited with a White Light Laser (WLL ...
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bioRxiv - Microbiology 2020Quote: ... and 3x 100%) was then used to remove all water from the biofilms before they were critical point dried (Leica CPD 030, Austria).
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bioRxiv - Cancer Biology 2022Quote: ... acquired with a either a 20x water-immersion objective or a 63x oil-immersion objective on a laser-scanning confocal microscope (Leica Microsystems; TCS SP5) operated with LAS software ...
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bioRxiv - Plant Biology 2022Quote: All imaging was performed using a Leica SP8 FALCON confocal microscope equipped with a 20x or 40x water immersion lens (Leica Microsystems, Mannheim, Germany). A White Light Laser (WLL ...
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bioRxiv - Evolutionary Biology 2023Quote: Rehydrated brains were embedded in agarose gel (5% UltraPure agarose power in deionised water) and sectioned using a vibrating blade microtome with an 80 µm interval (Leica VT1200, Wetzlar, Germany). Sections were then washed six times in PBS-TX (0.5% Triton X-100 detergent in phosphate-buffered saline) ...
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bioRxiv - Plant Biology 2023Quote: ... The section was removed from the staining solution and mounted in deionized water for visualization using Leica TCS-SP8 microscope (Leica microsystems, Wetzlar, Germany).
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bioRxiv - Plant Biology 2023Quote: ... for 5 min to stain cell walls and/or mounted directly in water for imaging on a Leica SP8 confocal microscope (Leica Microsystems, Wetzlar, Germany). Image analysis was done using Fiji.
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bioRxiv - Neuroscience 2023Quote: ... or upright laser-scanning two-photon microscope (Bruker Ultima) with a 20X water immersive objective (respectively Leica NA 1.0 and Zeiss NA 1.0). The tdTomato was excited by 552 nm laser wavelength on the confocal and 1040 nm on the two-photon ...
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bioRxiv - Biophysics 2020Quote: ... The image is obtained using ×25 or ×40 water immersion objective on a TCS-SP5 confocal laser scanning microscope (Leica Microsystems Inc., IL, USA).
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bioRxiv - Bioengineering 2023Quote: ... EC Plan-Neofluar 40x/1.30 Oil DIC M27) and a 25x water immersion long-working distance objective lens (Leica, NA 0.95, WD = 2.5 mm), the latter one was mounted on a custom mounting thread ...
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bioRxiv - Biophysics 2020Quote: ... Laser scanning is performed using a pair of galvanometric mirrors (Cambridge Instruments).The beam is expanded using a telescope to overfill the objective back aperture (Leica 63x, 1.2 NA, water immersion) and is directed into the objective using a dichroic mirror (Semrock FF685-DI02-25X36) ...
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bioRxiv - Cell Biology 2020Quote: ... The light is focused onto a small confocal volume through a 63x water immersion objective lens with NA=1.2 (Leica HC PL APO 63x/1.20 W CORR). The signal is detected by sensitive detectors (Leica HyD SMD ...
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bioRxiv - Genetics 2024Quote: ... and washed 3x 5 min with embryo water before being embedded in 1% low melting agarose (containing 1:25 tricaine) for confocal live imaging (Leica SP8, 10X air objective, NA=0.4). Average Z-projections were generated ...
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bioRxiv - Developmental Biology 2024Quote: ... We then imaged the exposed region of the midgut us-ing an upright Leica SP5 multi-photon confocal microscope with a 20x water immersion objective (Leica HCX APO L 20x NA 1.0). We fed flies via a microcapillary feeder tube throughout the entire imaging process ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were imaged on a Leica Stellaris 8 confocal microscope using a 20x/0.7 NA water objective (Leica HC PL APO 20x/0,7 IMM CORR CS2). 3D stacks of the dentate gyrus (581 μm x 581 μm x 30 μm ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were imaged on a Leica Stellaris 8 confocal microscope using a 20x/0.7 NA water objective (Leica HC PL APO 20x/0,7 IMM CORR CS2). Tile images spanning the hippocampus were acquired at voxel sizes of 142 nm x 142 nm x 300 nm ...
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bioRxiv - Neuroscience 2024Quote: ... NeuN and active caspase-3 signals in dLGN (Figure S4C) were imaged using a 20x/0.7 NA water objective (Leica HC PL APO 20x/0,7 IMM CORR CS2). A 590 nm laser and an emission read of 595 nm – 750 nm were used for imaging caspase-3 and a 499 nm laser with a 509 nm - 595 nm emission read was used for the NeuN signal ...