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Citations for Leica :
1 - 50 of 340 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... All slides preparation was performed using RNA free water and RNAse protocol from Leica autotec system.
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bioRxiv - Plant Biology 2020Quote: ... on RNase-free polyethylene naphthalate PEN membrane slides (Leica). The slides were dried at room temperature and deparaffinized with 100% xylene.
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bioRxiv - Plant Biology 2021Quote: ... 9.3% water in water-free acetone in a Leica AFS 2 (Leica Microsystems, Wetzlar, Germany) as described previously (Flechsler et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Transversal sections with a thickness of 8μm were cut on an RNase-free microtome (RM2255, Leica, Germany) and mounted on a cut edge frosted glass slide (VWR ...
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bioRxiv - Physiology 2024Quote: ... and rehydrated with ethanol and distilled water using a histological automaton (Leica Biosystem, cat#ST5020). Antigen retrieval was performed by submerging the slides in preheated distilled water ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating vibratome (Leica VT1000) sections of 70 μm were obtained the day of perfusion-fixation ...
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bioRxiv - Neuroscience 2020Quote: ... In short: tissue blocks were sectioned at 10 μm in an RNAse free cryostat and mounted on PEN-slides (Leica). Immediately after mounting ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... distilled water using a MZ205 FA fluorescent dissection microscope with a 2x PLANAPO objective lens (Leica) and LasX software (Leica) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were washed again with distilled water and imaged (Leica Microsystems Model-DMIL LED Fluo) to determine the colony size and area ...
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Bioengineering 2023Quote: ... rinsed in distilled water and dehydrated in a graded ethanol series before clearing in xylenes and infiltrating with paraffin wax (Leica). The resulting formalin fixed paraffin embedded (FFPE ...
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bioRxiv - Bioengineering 2023Quote: ... A biotin-free polymeric IHC detection system (Leica DS9800) consisting of HRP conjugated Gt anti-Rb IgG secondary antibody was applied for 25 minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... the conidia were harvested using distilled water containing 0.02% Triton X-100 and counted using a hematocytometer under a Leica DM500 microscope (Leica, Wetzlar, Germany).
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bioRxiv - Plant Biology 2023Quote: ... The samples were rinsed with double distilled water and dehydrated in series of ethanol and dried in CPD (Leica EM CPD300), gold coated ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... brains were sliced using a free-floating vibratome (Leica VT1000S) at 75 μm thickness ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Free-floating sections (50 μm) were cut using a vibratome (Leica VT1200 S ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating sections (30 µm) were obtained using a cryostat (Leica). For immunostaining ...
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bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were placed onto PEN membrane slides (RNAase-Free, Leica, Germany). All sections were kept on ice before imaging.
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bioRxiv - Bioengineering 2022Quote: ... which were confirmed to be free of damage by visual inspection (Leica S APO stereomicroscope ...
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bioRxiv - Neuroscience 2020Quote: ... cut into free-floating sections (30 μm) with a vibrating microtome (Leica) and kept at −20°C in a solution containing 300 mL.L−1 ethylene glycol ...
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bioRxiv - Neuroscience 2024Quote: ... 50µm coronal free-floating sections were obtained on a vibratome (Leica VT1000) in cold PBS and stored at 4°C in PBS for up to one week ...
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bioRxiv - Neuroscience 2021Quote: ... Free-floating coronal sections (40 μm) were cut with a cryostat (Leica, Germany), permeabilized with PBS containing 0.3% Triton X-100 (PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... Consecutive 30μm-thick free-floating sections were collected in a cryostat (Leica CM3050S) and stored in cryoprotectant at −20°C until further processing for immunofluorescence.
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bioRxiv - Neuroscience 2021Quote: ... Free-floating sections (30μm thickness) were cut using a cryostat (Leica, Wezlar, German) and placed in a cryoprotectant solution (sucrose 30% ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... Free-floating coronal sections (30 µm) obtained using a cryostat (Leica CM 1510S) were used for immunohistological investigations.
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bioRxiv - Neuroscience 2023Quote: ... to section (40 μm free-floating) using a cryostat (Leica CM1850; Leica Biosystems). Sections were serially collected into physiological buffer solution (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... to section (40 μm free-floating) using a cryostat (Leica CM1850; Leica Biosystems). Sections were serially collected into physiological buffer solution (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Free-floating brain sections (30 μm) were cut in a cryostat (Leica CM1950). Tissue sections were washed several times in PBS and blocked with 0.1% Triton X-100 and 10% goat serum for 1 h at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Free-floating coronal sections (40 µm) were prepared with a cryostat (Leica, Germany). For confirmation of cell-type-specific viral labelling ...
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bioRxiv - Neuroscience 2021Quote: ... 25 µm thick free-floating coronal sections were cut using a vibratome (Leica VT1000S). For cryosections ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sectioning was performed at −20 °C on a Cryostat Microtome (Leica, Germany) to generate 30 μm-thick coronal sections ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal free floating sections (50 μm) were generated using a vibratome (Leica; VT 1000S) collected every 4th sections in a tube containing a cryoprotective medium (polyvinyl pyrrolidone 1% w/v in PBS/ethylene glycol 1:1 ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal free floating sections (50 μm) were generated using a vibratome (Leica; VT 1000S) and stored at −20°C in a cryoprotective medium (polyvinyl pyrrolidone 1% w/v in PBS/ethylene glycol 1:1) ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sagittal sections (40 μm) were cut using a cryostat (Leica CM1950, Leica). Sections were first washed in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sagittal sections (40 μm) were cut using a cryostat (Leica CM1950, Leica). Sections were first washed in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty μm thick free-floating sections were cut using a cryostat (Leica, Wezlar, German), stored in at −20°C in sucrose 30% ...
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bioRxiv - Neuroscience 2021Quote: ... Four series of 30μm free-floating sections were obtained with a cryostat (Leica CM3050S) in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... and lesion-free brain tissues were snap-frozen and sectioning using a cryostat (Leica). Specimens were air dry for 15 min and fixed in 4% PFA at room temperature for 15 min and washed three times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating tissue sections were cut using a vibratome (VT-1000S, Leica Microsystems, Germany). After washing in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were obtained and sliced in RNAase-free PBS using a microtome (Leica VT1200), to obtain 1-2 slices containing the prefrontal cortex (AP 1.3 to 2.0) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was sectioned free floating in cold 1X PBS using a vibratome (Leica VT1200S). Brain tissue was sectioned at 70 μm thickness and spinal cord tissue was sectioned at 80 μm thickness.
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bioRxiv - Neuroscience 2024Quote: ... 40-60 μm free-floating brain sections were prepared with a vibratome (Leica VT1200) and immuno-labeled with primary antibodies (aSyn ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen brains were sliced into 40μm-thick free-floating coronal sections using a cryostat (Leica). Brain sections were blocked with 1% bovine serum albumin (BSA) ...