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Citations for Leica :
251 - 300 of 409 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The lumbar spinal cord (L1-5) was sectioned transversely (300µm) in dissection solution using a vibrating microtome (Leica Microsystems). Slices were immediately transferred to recording artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then cut into 350 μm transverse slices in the same solution using a vibratome (VT1200S, Leica). The slices were then transferred to recovery aCSF (in mM ...
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bioRxiv - Genomics 2024Quote: ... and once in 95% ethanol before being introduced to alcoholic eosin (Eosin Y Solution, Alcoholic, Cat No. 3801615, Leica). After cytoplasmic staining was completed ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed for 20 min with Bond Epitope Retrieval Solution #2 (Leica Biosystems, equivalent EDTA, pH 9.0). Primary antibody (dilution at 1 ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in an ice-cold choline solution and 300 μm-thick slices were obtained using a vibratome (Leica Biosystems VT1200S). The cutting solution contained the following (in mM) ...
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bioRxiv - Neuroscience 2021Quote: Samples were placed atop the frozen solution and the cryotubes were transferred to an AFS2 freeze substitution unit (Leica Microsystems). Samples were held at -90° for 45 hours ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... and coronal sections (250 μm) containing OFC were cut in ice-cold NMDG solution using a vibratome (Leica, Nussloch, Germany). Slices were then allowed to recover in warm NMDG solution (32°C ...
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bioRxiv - Neuroscience 2021Quote: ... and the cerebellum was cut into 250 μm thick parasagittal slices in the same solution on a vibratome (VT1200S, Leica). Slices were then transferred to 34°C warm artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2021Quote: ... coronal brain slices (250 μm) containing M1 forelimb regions were prepared in the same slicing solution using a vibratome (VT1200S; Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were quickly frozen in embedding solution (Sakura Finetek) and cut into coronal sections using a cryostat (CM3050, Leica) at a thickness of 50 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... IHC technique was performed with commercial solutions from the Novolink™ Max Polymer Detection System Kit (Leica Biosystems, Newcastle UK). Before antibody incubation ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2020Quote: ... Adrenal gland slices were allowed to recover for 15 min at 37°C in bicarbonate-buffered saline in the presence of 0.2 mM (+)-tubocurarine and then kept at room temperature in the same solution before cryofixation in external cryoprotectant (20% bovine serum albumin in bicarbonate-buffered saline) using a HPM100 HPF device (Leica). Freeze substitution was performed as previously published (Rostaing et al. ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... 300-μm coronal brain stem slices were prepared in carbonated ice-cold slicing solution using a vibratome (Leica VT 1200S) and allowed to recover for 20 min at 33 °C in carbonated high-osmolarity artificial cerebrospinal fluid (high-Osm ACSF ...
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bioRxiv - Biophysics 2022Quote: ... was mixed quickly with the ATP solution on the grid before blotting for 1 s in an EM GP2 grid freezing device (Leica) at 4 °C and 100% humidity and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Neuroscience 2020Quote: ... The injury section was further cut into 30 μm sections and mounted onto a series of 10 slides coated with Silane Surgipath Solution (Leica). These slides were then stored at −80° C ...
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bioRxiv - Plant Biology 2021Quote: ... The stem segments were embedded in 8% agarose solution and sectioned with a VT100 S vibratome (Leica, https://www.leica-microsystems.com/). Some of the cross-sections were used for FT-IR analysis and the others were stained with a FASGA staining solution prepared as described in Tolivia and Tolivia (1987 ...
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bioRxiv - Neuroscience 2021Quote: ... and continuously perfused with HEPES-based Ringer’s solution via am VC 8 perfusion system (Warner Instruments, Cat. No.640186) directly triggered by the recording software (Leica). Images were acquired at 9.8 Hz at a resolution of 512 x 300 pixels using a 488 nm laser in the resonant ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were then decapitated and the brain was isolated in the same sucrose solution and cut on a slicing vibratome (Leica, VT1200S ...
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bioRxiv - Cancer Biology 2022Quote: ... Heat-induced epitope retrieval was performed using citrate-based BOND Epitope Retrieval Solution 1 (Leica Biosystems, Buffalo Grove, IL, USA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and horizontal slices (300 µm) were cut in ice-cold NMDG-based solution using a VT1200 S vibratome (Leica). Slices were then held in NMDG-based solution maintained at 35°C for ∼12 minutes before being transferred to a modified artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cell Biology 2021Quote: ... The tubing was set to allow pumping of the solutions through the heart (attached to a perfusion needle (Leica, 39471024)) into the vasculature with the same entry point used for PBS and PFA perfusion steps described above ...
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bioRxiv - Pathology 2021Quote: ... tissue was subjected to automated baking and deparaffinization followed by heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) at 100 °C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Immunology 2021Quote: ... brains were removed from control and DSS-treated mice and placed in cold Gey’s buffer solution before slicing 200 μm slices using a vibratome (Leica VT1000S). Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size ...
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bioRxiv - Neuroscience 2023Quote: ... until the brains sank in the solution (1-3 days) and at which point 35um thick hypothalamic sections were obtained using a cryostat (Leica). Sections were first incubated for 1 hour in blocking buffer (1X PBS with 2% BSA and 0.1% tween-20) ...
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bioRxiv - Neuroscience 2022Quote: ... brains were rapidly removed and coronal slices (300 μm) containing the right AC were prepared in a cutting solution at 1 °C using a Vibratome (VT1200 S; Leica). The cutting solution ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed in the same solution and 40 μm-thick coronal slices were cut at the vibratome (Leica). Two PMv coronal sections per animal were selected to perform c-fos immunostaining as follows ...
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bioRxiv - Neuroscience 2024Quote: ... Transverse hippocampal slices (300 µm for whole-cell recording while 400 µm for fEPSP recording) were cut in the cutting solution using a vibratome (VT-1200S, Leica) and transferred to artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2024Quote: ... The brains (five brains were pooled for each sample) were collected in ice-cold KREBS solution and sliced into 300 μm sections using a vibratome (VT1000S Leica). The PMBSF and ALBSF territories were rapidly microdissected under a stereo microscope ...
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bioRxiv - Neuroscience 2023Quote: ... All brains were then flash frozen in OCT solution and cut at 100um thickness using a cryostat (Leica CM 1950) (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... we sectioned half-brains at 25 µm thickness on frozen 30% sucrose solution slabs on a sliding microtome (Leica SM2000R) equipped with freezing stage ...
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bioRxiv - Neuroscience 2023Quote: ... Braun) to a sylgard-coated cover glass covered with bath solution (Sylgard 184, Dowcorning) under a stereoscopic microscope (M50, Leica). After clearing the viscera by suction through a glass pipette ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were subjected to automated heat-induced epitope retrieval using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) for 20 min at 100°C ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Plant Biology 2022Quote: ... roots were dipped in propidium iodide (PI) solution (10 µg/ml) for 30 secs to stain the root cell wall (Leica SP8 confocal microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed samples were dehydrated in a series of graded alcohol solutions and embedded in paraffin blocks using a Leica TP 1020 automatic tissue processor (Leica). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... animals were rapidly decapitated and the brain of each animal was removed and cut parasagittally in ice-cold cutting solution on a Leica VT1000S vibratome (Leica Microsystems Inc ...
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bioRxiv - Microbiology 2024Quote: ... and subjected to automated baking and deparaffinization followed by heat-induced epitope retrieval (HIER) using a ready-to-use EDTA-based solution (pH 9.0; Leica Biosystems) at 100 °C for 15 min ...