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Citations for Leica :
151 - 200 of 409 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Horizontal slices were prepared in the same cutting solution with a vibratome (Leica VT1200S) and incubated for 45min-1hr at 32°C in a solution containing (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were subsequently sectioned in the same solution using a vibrating microtome (Leica, VT1200S). The brain sections were left to recover for 30 min at 37°C in carbongenated standard aCSF ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections for F4/80 staining underwent epitope retrieval using enzyme 1 solution (AR9551, Leica) for 10 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... antigen retrieval was performed by immersion in Epitope Retrieval Solution pH 6 (Leica Biosystems) and incubated at 60°C in an oven for 18 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... employing BOND Epitope Retrieval Solutions and the BOND Polymer Refine Detection kit (Leica Biosystems) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were cut in ice-cold sucrose solution using a vibrating microtome (Leica VT1200 S), and were left to recover for 15 min at 32°C before being transferred to a standard artificial CSF (aCSF ...
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bioRxiv - Cell Biology 2020Quote: ... Brain images were acquired in a 4% PFA solution using a stereomicroscope (M80, Leica Microsystems).
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bioRxiv - Neuroscience 2020Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... Heat-induced epitope retrieval was done for 20 minutes with ER2 solution (Leica Microsystems AR9640). All the experiment was done at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by decalcification of bone in a formic acid solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a Leica ASP6025 tissue processor ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides underwent heat-induced epitope retrieval with Epitope Retrieval Solution 2 (pH 9.0, Leica AR9640) at 95°C for 5 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... Heat-induced epitope retrieval was performed for 20 min with ER2 solution (Leica Microsystems AR9640). All the experiments were performed at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue sections were first deparaffinized prior to EDTA-based antigen retrieval (Leica ER2 solution, 20min). A cyclical staining protocol was then performed ...
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bioRxiv - Immunology 2022Quote: ... containing 30% glycerin solution and imaged on a Leica SP8 confocal microscope (Leica Microsystems, Germany). Bone surface expressing TRAP was quantified using Imaris Software (Oxford Instruments ...
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bioRxiv - Plant Biology 2023Quote: ... fluorescein solutions at 10 µM were imaged with a 10x PLAN APO dry objective (Leica) at λex = 488 nm and λem = 501-609 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by an initial antigen retrieval using Epitope retrieval solution 2 (Leica Bond ER2, #AR9640) at 95°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Each staining step was followed by subsequent washing steps with Wash solution (Leica Bond, #AR9590).
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bioRxiv - Neuroscience 2023Quote: ... then moved to a 30% sucrose solution before sectioning with a sliding microtome (Leica SM2010R) at a thickness of 30 µm ...
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bioRxiv - Cancer Biology 2024Quote: ... for transferrin receptor 1 (TfR1) and using Bond Epitope Retrieval Solution 2 (Leica Biosystems AR9640) for Ki67 and CD31 ...
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bioRxiv - Neuroscience 2024Quote: ... and then high pressure frozen in the same solution (Leica MicroSystems ICE high pressure freezer). They were then placed in plastic cryo tubes inside a low temperature embedding machine (AFS ...
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bioRxiv - Synthetic Biology 2024Quote: ... The motion of nanoparticles in mucus solution was recorded using a Leica SP8 (Leica, Germany) microscope equipped with a 40×water objective ...
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bioRxiv - Cell Biology 2020Quote: ... brains were removed and placed in the cold sucrose solution for sectioning (Leica VT 1000S Vibratome). Transverse ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were first permeabilized with heat in Bond Epitope Retrieval solution 2 (pH 9.0, Leica - AR9640) at 95°C for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100°C for 30mins ...
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bioRxiv - Cell Biology 2021Quote: ... followed by heat induced antigen retrieval performed with Epitope Retrieval Solution 1 BOND (Leica, Wetzlar, Germany). PCNA Primary antibody was diluted 1:500 (Abcam ...
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bioRxiv - Plant Biology 2022Quote: ... anthers were stained with 20 μg/ml propidium iodide solution and examined using TCS SP5 (Leica) or ZEISS 710 confocal microscopes with excitation at 514 nm and emission at 526-560 nm for mVenus ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in 30% sucrose solution overnight and finally embedded in Tissue OCT compound (Leica, Wetzlar, Germany). Sections of 10 μm thickness were cut on a cryostat (CM 1900 ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
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bioRxiv - Systems Biology 2023Quote: ... tissue sections were first deparaffinized prior to EDTA-based antigen retrieval (Leica ER2 solution, 20 minutes). A cyclical staining protocol was then performed ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μm coronal VTA sections were cut in the same solution using a VT1000s vibratome (Leica). The VTA from each section was dissected using a fine scalpel blade under a dissecting microscope ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica, cat# AR9640). Both mouse monoclonal anti-Ki67 CloneMIB-1 (Dako ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical analyses were performed utilizing ready-to-use Ki67-specific antibody solution (K2, Leica Biosystems, PA0230). Sections were dewaxed ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Neuroscience 2023Quote: ... and glued to a platform containing ice-cold NMDG slicing solution within a vibratome (VT1200, Leica). Coronal sections (300 µm ...
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bioRxiv - Cancer Biology 2024Quote: ... Heat-induced epitope retrieval was performed using citrate-based BOND Epitope Retrieval Solution 1 (Leica Biosystems) for BRG1 ...
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bioRxiv - Neuroscience 2024Quote: ... ice-cold cutting solution using a sapphire blade (Delaware Diamond Knives) on a vibratome (VT1200S; Leica). Slices containing the dLGN and OT were allowed to recover at 30°C for 15 minutes in oxygenated saline solution (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... These fragments were placed individually on top of drops of sucrose solution on aluminium EM pins (Leica) and immediately frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... washed once with live cell imaging solution and imaged using an inverted fluorescence microscope (DMI 3000, Leica).
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bioRxiv - Neuroscience 2020Quote: ... the sucrose solution was removed and organoids were cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 50 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... the sucrose solution was removed and organoids were cryosectioned using a standard cryostat (Leica CM1860, Leica Biosystems). 50 μm thick sections were collected on Ultra Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For both antibodies antigen unmasking was undertaken using Heat Induced Epitope Retrieval (HEIR) solution 1 (Leica AR9961) for 30 minutes at 100°C.
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bioRxiv - Plant Biology 2021Quote: ... The tissue was then embedded into blocks with an embedding solution of activated historesin (Leica, Wetzlar, GER), hardener (barbituric acid ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was quickly removed and slices were prepared in cutting solution using a vibratome (VT1000A, Leica, Leica Biosystems GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... V1 coronal slices (350 μm thick) were prepared in the same solution with a VT1000S vibratome (Leica). Slices were then stored and allowed to rest at room temperature for an hour in a holding chamber filled with artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Microbiology 2022Quote: ... femur and tibia were decalcified in a formic acid and formaldehyde solution (Surgipath Decalcifier I, Leica Biosystems). Tissues were then processed in ethanol and xylene and embedded in paraffin in a tissue processor (Leica ASP6025 ...
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bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was done by incubation with Bond Epitope Retrieval solution 2 (AR9640, Leica Biosystems, Wetzler, Germany) for 20 minutes and then sections were then washed with Bond Wash solution 3x ...