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Citations for Leica :
201 - 250 of 425 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 100x objective using a high-resolution light microscope (Leica DM6000 BD, Leica Microsystems, Wetzlar, Germany). For morphometric analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then embedded in low melting point agarose (σ Type VIIA, 3% in Ames medium) and 250 µm slices were obtained using a vibratome (Leica Microsystems, VT1200S) in cold (∼4° C) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples of GN2 or scraped cells were placed between two 3 mm diameter aluminum- or gold-coated (Type A) specimen carriers with 100 µm indentations (Leica Microsystems Inc., Austria). HT29 cells directly grown on the same type of 3 mm diameter aluminum- or gold-coated (Type A ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle sections were also immunostained with human-specific spectrin (SPEC1-CE, 1:50, Leica) and human-specific lamin A/C (ab40567 ...
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bioRxiv - Immunology 2024Quote: ... ýIII-Tubulin (1:250) and human HLA-DR (1:250, LN-3, Leica/Novocastra) followed by staining with a cocktail of DAPI and fluorochrome-conjugated secondary antibodies and mounting on histological slides.
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bioRxiv - Neuroscience 2020Quote: ... which shows expression in uninjured axons.25 All immunostains were performed using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The entire cerebellum of each animal (N=5 analyzed) was sectioned coronally at 50μm thickness on a vibratome (Leica VT 1000S) and all sections were processed for immunohistochemistry using a human nuclear antigen antibody (HuNu ...
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... were prepared from Sprague Dawley rats (n=3; 3-4 weeks old; Janvier Laboratories) and cut using a vibratome (Leica VT1200S) in an ice-cold oxygenated ...
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bioRxiv - Cancer Biology 2024Quote: ... We generated a union of p53R172H peaks (n= 7,721) from two sets of peaks called with two p53 antibodies (Epicypher p53 antibody and Leica p53 antibody). As a control ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: to detect muscle fibre types we used mouse anti- myosin heavy chain (slow) (Leica, clone WB-MHCs; 1:200); to delimit the fibres we used rabbit anti-collagen type VI (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... pollen grains from mutants and wild type were cultures on the same medium for 3h/ 6h and observed using a microscope under brightfield (DM4000B, Leica; Liu et al., 2018).
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bioRxiv - Cell Biology 2022Quote: ... One 5um section from each tissue type was stained with hematoxylin and Eosin (H&E) and scanned at 20X magnification using an Aperio AT2 (Leica Biosystems, Buffalo Grove, IL) into whole slide digital images.
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bioRxiv - Neuroscience 2020Quote: Quantification of mitochondria transfer events was obtained from randomized n≥ 12 brain ROIs acquired using a confocal microscope (Leica TCS SP5 Microscope) and data are expressed as % ± SEM of fGFP-/MitoDsRed+ particles over total fGFP-/MitoDsRed+ particles found in GFAP+ astrocytes ...
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bioRxiv - Cancer Biology 2020Quote: ... Semi quantitative analysis was done by counting three independent microscopic fields (n=100-200 cells/field) for staining of specific antigen using upright microscope (Leica DM1000, Germany). Tissue array (US Biomax ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were observed using an objective lens (N Plan 5x/0.12 PH 0) on a Leica DM IL LED fluorescence microscope (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Perfused brains (n = 20 / sex; 40 total) were sliced coronally into 6 series at 30 µm on a vibrating microtome (Leica VT 1000S). The samples were stored at -20 °C in cryoprotectant until immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2024Quote: ... n = 7 mice/21 slices for GluN1-Ab) were prepared in ice-cold paCSF on a vibratome (VT 1200S, Leica, Wetzlar, Germany) and transferred for warm recovery in paCSF at 33°C for 12 min ...
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bioRxiv - Cell Biology 2024Quote: ... adjusted pH 7.25–7.3 with 10 N NaOH to set Na 154 mM) using Leica VT1200 S fully automated vibrating-blade microtome (Leica, Wetzlar, Germany). Sections were kept at 37 °C for 20 min and then allowed to return to room temperature for the duration of the experiment ...
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bioRxiv - Neuroscience 2023Quote: Live organoid images were acquired on a Leica DMI6000 inverted microscope utilizing either 5x or 10x objectives (5x Leica N Plan, NA = 0.12, 10x Leica PL Fluotar, NA = 0.3). Excitation/emission filters for ZsGreen were BP 480/40nm and BP 527/30nm ...
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bioRxiv - Neuroscience 2024Quote: Fresh-frozen brain tissue samples from the three groups (n=3) were sectioned at 10 μm thickness using a cryostat (Leica Instruments GmbH, Germany). Tissue optimization was performed with TO slides (part no ...
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bioRxiv - Cell Biology 2020Quote: Imaging of the mouse and human sections was carried with the DM5500 fluorescent microscope (Leica Microsystems). Images were captured with the Leica application suite X version ...
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bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Attached RONAs and human cortical assemblies were sectioned (25 μm) by cryostat (CM3050; Leica, Nussloch, Germany) and collected on SuperFrost Plus glass slides (Roth ...
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bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Neuroscience 2022Quote: Paraffinized human optic nerves were cut in 5 μm sections using the microtome RM2155 (Leica, Wetzlar, Germany) and placed on 1 mm thick microscope slides (Marienfeld ...
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bioRxiv - Immunology 2023Quote: Platelet interactions with human monocytes were imaged in a Leica TCS SP5 SMD confocal system (Leica Microsystem). Cells were seeded in microslide 8-Well IBIDI chamber ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: PFA fixed human postmortem brain tissues (PFC region) were sectioned at 30µm using a cryotome (Leica SM2010R). Sections were then rehydrated and washed in 1X sterile PBS and permeabilized using 1X sodium citrate buffer pH 6.0 (heated at 95°C for 10 mins) ...
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bioRxiv - Neuroscience 2022Quote: Human and mouse brain tissue was sectioned (30 μm-thick sections) using a SM2010 R sliding microtome (Leica) equipped with a BFS-3MP freezing stage and cooling unit (Physitemp ...
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bioRxiv - Cancer Biology 2020Quote: ... and a detection kit (Bond Polymer Refine Red Detection Kit; Leica Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Immunology 2022Quote: ... serial human kidney sections were stained with anti-CD3 (LN10) or anti-CD8 (4B11; both Leica Biosystems, Chicago, IL) antibodies ...
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bioRxiv - Cell Biology 2021Quote: Fixed and stained human pancreatic tissue sections were examined using a commercially available DragonFly200 spinning disk system (Andor) on a DMi8 microscope (Leica) with Plan Apo 40× NA 1.3 and Plan Apo 63× NA 1.4 oil immersion objectives ...
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bioRxiv - Cancer Biology 2021Quote: All staining of human tissues was performed on a Leica Bond RX automated stainer (Leica Microsystems, Inc., Buffalo Grove, IL). To ensure lectin specificity ...
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bioRxiv - Neuroscience 2023Quote: ... The entire human DRG section was imaged and merged using Leica Application Suite X (LAS-X, v.3.7, Leica Microsystems). Acquisition setting were kept consistent throughout imaging across different experimental days ...
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bioRxiv - Bioengineering 2022Quote: ... Bio SB) or anti-human CD163 (clone EP324, Bio SB) followed by an HRP-conjugated anti-mouse secondary antibody (Leica) and revealed with the peroxidase substrate 3 ...
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bioRxiv - Bioengineering 2022Quote: ... The human-distal region skin tissue was cryoprotected with 30% sucrose and continually sectioned (thickness: 50 µm) using a cryostat (Leica CM 1950 ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to analyze 34 human tumor samples on the Leica Bond RX automated system (Leica Biosystems, Buffalo Grove, IL) as per manufacturer’s protocol23 ...
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bioRxiv - Cancer Biology 2024Quote: ... A polymeric kit (Refine, Leica) was used as secondary reagent for detection ...
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bioRxiv - Cell Biology 2021Quote: Cryo-samples prepared from single-tagged CLTA-GFP expressing SK-MEL-2 cells and incubated with Alexa Fluor® 647 ChromPure human transferrin (Jackson Immuno Research Inc.) were imaged on a Leica EM Cryo CLEM system (Leica). The system consists of a Leica DM6 FS widefield microscope that is equipped with a motorized Leica EM Cryo stage and a short working distance (<0.28 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... In regions with CCF two to four consecutive cryosections with a thickness of 12 µm were put on membrane-slides (Leica Frame Slides, Nuclease and human nucleic acid free, PET-Membrane, 1,4 µm, Leica, Wetzlar, Germany) and incubated for one minute in 70% ethanol at −16°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Physiology 2024Quote: Human cells (A549 and BEAS-2B) were investigated using the TCS Sp5 Inverted Confocal laser scanning microscope (Leica Microsystems, Wetzlar, Germany) and a 20x /0.70 HC Plan Apochromat ...
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bioRxiv - Neuroscience 2024Quote: FFPE blocks of post-mortem human midbrains were sectioned at 6 µm thickness using a rotary microtome (HistoCore MULTICUT R Rotary, Leica Biosystems). The sections were mounted on Fisher Scientific slides (catalog #12-550-15 ...
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bioRxiv - Neuroscience 2024Quote: FFPE blocks of human midbrains containing substantia nigra (SN) were cut on a rotary microtome (HistoCore MULTICUT R Rotary, Leica Biosystems) at 6 µm ...