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Citations for Leica :
151 - 200 of 425 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... 5-day-old wild-type Arabidopsis roots or 3-week-old true leaves were frozen in a high-pressure freezer (EM ICE, Leica), followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2 ...
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bioRxiv - Bioengineering 2024Quote: The specimens were immersed in an external cryoprotectant solution (1-hexadecene) and gently placed into HPF sample carriers (depth: 0.2 mm, Type A, Leica 16770141). The cover (Type B ...
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bioRxiv - Systems Biology 2024Quote: ... All data were obtained with a Nikon 100x Plan Apo (NA 1.4) objective in combination with Leica Microsystems immersion oil type F (Leica, Germany) using a Photometrics Prime sCMOS camera and a CoolSNAP HQ2 camera (Photometrics ...
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bioRxiv - Cell Biology 2024Quote: ... Worm pellets were resuspended in 0.15 M sucrose prepared in M9 buffer and loaded into 200 µm deep well of a A-type carrier (Leica). The assembly was covered with flat side of B-type carrier (Leica ...
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bioRxiv - Developmental Biology 2021Quote: ... Water immersion 10× objectives were used in both systems: N-Achroplan 10×/0.30 NA (Zeiss) and HC APO L 10×/0.30 NA (Leica), respectively ...
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bioRxiv - Molecular Biology 2020Quote: Laser powers used were measured behind the objective lens (N PLAN L 20s/0.40, Leica Microsystems, Wetzlar, Germany) with a LabMax-TO laser power meter equipped with an LM-2 VIS sensor (Coherent ...
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bioRxiv - Cancer Biology 2024Quote: ... dried at 37°C O/N and then processed with Bond-RX fully automated stainer system (Leica Biosystems) according to the following protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and processed on automated immunostaining systems (the Bond RX Leica for P-gP and Myh11, and the LEICA BOND III for SMA). Pictures were acquired using a slide scanner (Lamina ...
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bioRxiv - Molecular Biology 2024Quote: Pollen grains were mounted on slides in Alexander’s staining solution [Alexander 1969] and examined under the microscope (MZFL III Leica Microsystems, Wetzlar, Germany). Viable and non-viable pollen grains were observed in three independent experiments.
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bioRxiv - Developmental Biology 2024Quote: ... Fluorescence images of whole-mounted tongues and isolated papillae were obtained using a Leica MZ16FA stereomicroscope with a Fluo III fluorescence module (Leica, Heidelberg, Germany) and documented with a color digital CCD camera and image capture software (Q Imaging Retiga EXi camera and Q Capture software ...
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bioRxiv - Neuroscience 2024Quote: ... Injections were delivered using pulled glass pipettes (Drummond, 3.5”) in an injection system coupled to a hydraulic micromanipulator (Drummond, Nanoject III) on a stereotaxic frame (Leica, Angle Two™), at approximately 10 nl min−1 ...
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bioRxiv - Bioengineering 2021Quote: ... images were taken of a mixed population of wild-type worms on a seeded plate at 1.6x magnification on a stereomicroscope (Leica M165 FC) using a 1.3 MP CMOS camera (Thorlabs DCC1645C ...
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bioRxiv - Plant Biology 2021Quote: ... The root sections were mounted on slides in 50% glycerol. Wild type (cv. AMIGA) cluster root thin sections of 6 μm were produced using a microtome (RM2165, Leica Microsystems). They were counterstained for 5 min either with 0.05% toluidine blue or with 0.1% ruthenium red in 1X phosphate buffer saline (1X PBS pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: Left proximal tibia and distal femur samples from sex and age matched wild-type and knockout littermates were processed using a Leica TP1020 processor (Leica Biosystems) in preparation for methyl methacrylate (MMA ...
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bioRxiv - Genomics 2022Quote: ... ranging from 1 μg/mL to 5 μg/mL depending on tissue type and prepared for heat-induced epitope retrieval (HIER) on a Leica Biosystems automated tissue handler (Bond RX, Leica Biosystems). For cell pellet array (CPA ...
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bioRxiv - Biophysics 2024Quote: ... on top of which the samples (coverslips) were mounted using 10 μl of immersion oil (Type F immersion liquid, Leica Microsystems). After assembling the coverslip ...
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bioRxiv - Biophysics 2024Quote: ... Dissected egg chambers were high-pressure frozen in gold-coated copper Type A carriers (Engineering office M. Wohlwend) using the HPM010 (Wohlwend) or the EM Ice high-pressure freezing systems (Leica Microsystems). The egg chambers were added into the 100 µm or 200 µm cavity of the planchette and 20% Ficoll (70 kDa ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2024Quote: ... we sealed the gene frames with a glass coverslip and covered it with microscopy oil (type F immersion liquid, Leica microsystems). For each pair of species (P ...
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bioRxiv - Neuroscience 2022Quote: ... tissue biopsies were carefully placed inside and covered with the flat side of the lipid-coated specimen carrier type B (Leica, 16770153) and high-pressure frozen (∼2000 bar ...
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bioRxiv - Cell Biology 2024Quote: ... was transferred to B-type planchettes (3 mm) and rapidly frozen with a Leica EM ICE high-pressure freezer (Leica Microsystems). The frozen specimens were freeze-substituted in anhydrous acetone containing 2% osmium tetroxide (OsO4 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... covered by 6 mm type B carrier (side flat) and frozen with a high-pressure freezing machine (EM ICE, Leica microsystems) as previously described112 ...
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bioRxiv - Developmental Biology 2022Quote: ... Blastema tissues (n=3 for neotenic and metamorphic animals) were first embedded in a tissue freezing medium (Leica, 14020108926). Then ...
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bioRxiv - Bioengineering 2023Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ ...
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bioRxiv - Neuroscience 2023Quote: ... and cortex region (n=3/group) were cut at 5μm thickness in a rotary microtome (Leica RM2125RT, Wetzlar, Germany) for histological analysis (Venkidesh et al. ...
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bioRxiv - Bioengineering 2024Quote: ... Images (n = 5) of S-shape embedded filaments were taken with a confocal microscope (SPX8, Leica Microsystems, The Netherlands) and analyzed with ImageJ.
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bioRxiv - Cancer Biology 2021Quote: ... was done on sections of FFPE tissue using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA), with the following antibodies and conditions (dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on sections of formalin-fixed paraffin-embedded patient tissue (FFPE) using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA). Slides were de-paraffinized and heat-mediated antigen retrieval using the Bond Epitope Retrieval 1 solution at pH6 (H1 ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were dried overnight and then subjected to immunohistochemistry (IHC) using a modified protocol F on the Bond-Max III fully automated staining system (Leica Biosystems, Wetzlar, Germany). All reagents from the Bond Polymer Refine Detection Kit DC9800 (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... Human oocytes were imaged using a 63x water immersion objective (N.A1.20, Leica, 506346) with an incubation chamber maintained at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... The antibodies used were anti-human CD117 (EP10; Leica Biosystems, Buffalo Grove, IL), anti-human FcεRI (9E1 ...
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bioRxiv - Neuroscience 2023Quote: Human colonic tissues were embedded in paraffin using an embedding station (LEICA EG1150C) and sections (3 µm ...
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bioRxiv - Genomics 2024Quote: ... anti-human CD8 clone 4B11 dilution 1:30 (MA1-80231, Leica Biosystems, Invitrogen), anti-human CD4 clone SP35 dilution 1:30 (MA5-16338 ...
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bioRxiv - Plant Biology 2021Quote: ... The percentage root colonization was obtained by analyzing 10 random stained roots under microscope at 20X (Leica Microscope. Type 020-518.500, Germany). Staining was performed by softening the sample with 10% KOH for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... Organs of Corti from P34 mice were placed directly in aluminium specimen carriers with 0.2 mm cavity (type A, Leica Microsystems, Wetzlar, Germany). In a subset of experiments (P16 ...
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bioRxiv - Microbiology 2020Quote: ... Mock-infected S2 cells and S2 cells infected with wild-type CrPV or CrPV-DcDV were observed by bright-field microscopy using a Leica DM5000B microscope (Leica, Wetzlar, Germany).
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Microbiology 2020Quote: ... embedded in epoxy resin type TAAB 812 and sectioned (70 nm thick) using a Leica EM UC7 microtome (Leica Microsystems, Wetzlar, Germany). To find the area for transmission electron microscopy (TEM) ...
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bioRxiv - Neuroscience 2023Quote: ... The assembly was covered with a flat side of another B-type carrier and specimens were vitrified using a high-pressure freezing machine (Leica EM ICE).
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bioRxiv - Genetics 2021Quote: ... three sets of N ≥ 8 per genotype were pinched and scored for open wounds under a fluorescent stereo microscope (Leica MZ16FA with Planapo 1.6x objective and appropriate filters) ...
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bioRxiv - Immunology 2021Quote: Kidneys were isolated from resting mice after PBS perfusion and fixed O/N in 20 mL of 10% Neutral Buffered Formalin (Leica). Fixed kidneys were paraffin-embedded using a Shandon Citadel 1000 tissue processor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Slices were mounted onto a glass slice in 0.5 % n-propyl gallate/ 90 % glycerol/PBS (NPG) and the next day imaged by a confocal microscope (Leica Stellaris). Analysis of the dendritic spine density was performed in 3 different TTLL1WT and TTLL1KO brains (at least 10 labelled granule cell dendrites were analyzed per brain).
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bioRxiv - Neuroscience 2022Quote: ... while the remaining sections were selected for immunohistochemistry procedures using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL). Immunohistochemistry was performed for glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2020Quote: Five micron sections of tumors were immunolabeled with one of the following antibodies using a Leica Bond III automated immunohistochemistry instrument (Leica Biosystems, Buffalo Grove, IL): Rabbit anti-CD3 ...
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bioRxiv - Neuroscience 2023Quote: ... using Leica Bond III automated staining system with diaminobenzidine chromogen detection and the same antibodies as for clinical diagnostics (DS9800, Leica Biosystems, Buffalo Grove, IL). Hematoxylin and eosin stain was used for general histopathology ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Neuroscience 2022Quote: ... The epidermal layer was manually dissected from dermal and subdermal compartments of the skin sample and transferred into a type A aluminium specimen carrier (Leica Microsystems, Wetzlar, Germany) with recesses of 200 µm containing polyvinylpyrrolidon and capped with a second carrier without recess (Leica Microsystems ...
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bioRxiv - Immunology 2021Quote: ... The tissues were covered with the flat side of a Type-B brass planchette and rapidly frozen with an HPM-010 high-pressure freezing machine (Leica Microsystems, Vienna Austria). The frozen samples were transferred under liquid nitrogen to cryotubes (Nunc ...
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bioRxiv - Cell Biology 2021Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 40× and 100× objectives using a high-resolution light microscope (Leica DM6000 BD, Leica Microsystems, Wetzlar, Germany). In this analysis ...
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bioRxiv - Biochemistry 2023Quote: ... The tissue was sectioned to 10 μm using Cryofilm type 2C(9) tape (SECTION-LAB Co. Ltd., Yokohama Kanagawa, Japan) and a cryostat (Leica Biosystems, Wetzlar, Germany) while at -20 °C ...