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Citations for Leica :
1801 - 1850 of 1851 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: ... 30μm serial sagittal sections were collected from Cx3cr1GFP/+ animals in a tempero-medial manner (1 in 12 series) using a vibratome (Leica VT 1200S) and kept as free-floating sections in ice-cold PBS ...
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bioRxiv - Microbiology 2024Quote: ... followed by fine trimming of an initial region of interest (ROI) spanning 2 x 2 x 1 mm using a glass knife and Leica UC7 ultramicrotome (Leica Microsystems, Austria). The trimmed block was then mounted on an ultramicrotome stub using conductive silver epoxy (SPI ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...
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bioRxiv - Immunology 2021Quote: ... z-stacks were generated with a 20x objective at a z-step of 1 µm using a SP8 laser scanning confocal microscope (Leica Biosystems, Nußloch, Germany). Composite images were generated using ImageJ with Fiji distribution [31].
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Pathology 2021Quote: ... Slides were then stained with anti-Iba1 antibody (1:2000; Wako; 019- 19741 Richmond, VA) using the Bond Polymer Refine Red Kit (cat. DS9390 Leica Biosystem, Richmond, IL). The slides were stained using a pan-cytokeratin antibody (1:1000 ...
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bioRxiv - Bioengineering 2019Quote: ... Confocal laser scanning microscopy of macroMs entrapment/dead cells at 4h and day 1 was performed with a Tandem confocal system (Leica TCS SP8 STED), equipped with a white light laser (WLL) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RNAscope staining was followed by the C219 IHC at a 1:200 dilution for 30 minutes on a Bond RX auto-stainer using the Bond Polymer Refine Kit (Leica, Buffalo Grove, IL) minus DAB & Hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Neuroscience 2021Quote: images were obtained using the Axio Imager 1 microscope (Zeiss, Oberkochen, Germany) and confocal images were obtained with a laser scanning confocal microscope (Leica SPE, Mannheim, Germany) associated with a Leica LAS AF interface ...
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bioRxiv - Cell Biology 2020Quote: ... samples for the projects as listed in Table 1 were cryo-immobilized by using the following high-pressure freezers: EMPACT2+RTS (Leica Microsystems, Vienna, Austria), Leica EM ICE (Leica Microsystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Microbiology 2023Quote: ... Z stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (33) ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Microbiology 2023Quote: ... Z-stacks of horizontal plane images were acquired in 1 μm steps using a Leica SP8 AOBS inverted laser scanning microscope (CLSM, LEICA Microsystems, Wetzlar, Germany) at the INRAE MIMA2 platform (doi.org/10.15454/1.5572348210007727E12)54.54 ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... immersed in 1-Hexadecene as cryoprotectant and immediately fixed by high pressure freezing (HPF) using the Leica EM ICE (Leica Microsystems GmbH, Germany). Freeze substitution and embedding of the HPF-fixed samples were conducted in a temperature-controlled device ...
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Neuroscience 2020Quote: ... the brain of each mouse extending from anterior to the DG to the cerebellum (from 0.22 to −5.34 μm from Bregma) was sectioned at 30 μm in a 1:9 series using a freezing microtome (Leica SM 2000 R Sliding Microtome). Sections were stored in 1xPBS with 0.01% sodium azide at 4°C until processing for IHC ...
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bioRxiv - Plant Biology 2019Quote: ... the cotyledons were stained with 1 µg/ml propidium iodide and observed under a Confocal microscope (Carl Zeiss LSM710, Carl Zeiss LSM880 or Leica TCS SP8). For excitation ...
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bioRxiv - Cancer Biology 2021Quote: ... Agilent M087601-2, used at a dilution of 1/50 for 15mins at room temperature. HIER was performed on-board using Leica ER1 solution (low pH) for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the thecal plates of the dinoflagellates were stained with the fluorescent dye Calcofluor white M2R at a final concentration of 10–20 mg mL−1 (Fritz & Triemer, 1985) and examined under epifluorescence (lamp 50W) using a Leica DM IRB (Leica Microsystemps GMbH, Wetzlar, Germany) inverted microscope ...
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bioRxiv - Cancer Biology 2022Quote: Tissue sections were incubated for 1 hour in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections at 1 µm were prepared using the resin-embedded buds using a glass knife on an EM UC6 ultramicrotome (Leica Microsystems GmbH, Wetzlar, Germany). Sections were mounted on glass slides ...
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bioRxiv - Immunology 2024Quote: ... Sections were then stained for Ly6G (clone 1A8) at a previously optimized dilution (1/60000) for 30 min at room temperature with the autostainer Bond Rx (Leica Microsystems GmbH, Wetzlar, Germany). The IHC sections were visualised with 3,3’Diaminobenzidine (DAB) ...
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bioRxiv - Molecular Biology 2023Quote: ... Larvae were then positioned and oriented laterally to acquire two z-stacks of 30 optical sections (ΔZi, Zi-1 = 683.46μm) of the gastrointestinal tract using a 4X objective (Leica HC PL FLUOTAR 4X/0.13 DRY) at 10 msec exposures ...
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bioRxiv - Cancer Biology 2023Quote: Tissues sections were incubated for 1 h in primary antibodies and detected using the BOND Polymer Refine Detection System (DS9800; Leica Biosystems, Buffalo Grove, IL) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Lewy bodies were counted on sections from the described regions following immunohistochemistry for alpha-synuclein (Leica, cat# ncl-l-asyn, 1:100-400 dilution). Of note ...
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bioRxiv - Immunology 2023Quote: ... Frozen brains were then embedded in Epredia™ M-1 Embedding Matrix (Thermo Ficher Scientific) and cut in coronal sections using a cryostat (Leica CM3050S, Mannheim, Germany), and mounted on slides for either fluorescence immunocytochemistry (IF ...
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bioRxiv - Neuroscience 2024Quote: Immuno-stained anti-rabbit Iba-1 brain sections were imaged at a 40x oil objective using a confocal imaging system (Leica Microsystems, Deerfield, IL, USA) to further investigate microglia structure and morphology ...
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bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Cell Biology 2019Quote: Quantification of protein by Western blotting was carried out using standard techniques and the following primary and secondary antibodies: anti-α-SMA (SMA-R-7-CE, Leica Biosystems, dilution: 1:100), anti-COL1 (1310-01 ...
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bioRxiv - Cancer Biology 2021Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope by a senior pathologist (T.J.H.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Optimal primary antibody stripping conditions between rounds in the seven-color assay were performed following 1 cycle of tyramide deposition followed by heat-induced stripping (see below) and subsequent chromogenic development (Leica Bond Polymer Regine Detection DS9800) with visual inspection for chromogenic product with a light microscope (TH) ...
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bioRxiv - Molecular Biology 2023Quote: ... Zi-1 = 75.61μm) of pancreatic beta cells using the microscope’s TXR filter and a 40X objective (Leica HCX APO L U-V-I 0.80 W), at 20 msec exposures ...
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bioRxiv - Molecular Biology 2023Quote: ... three z-stacks of 75 optical sections each (ΔZi, Zi-1 = 151.75μm) were acquired of the bigger liver lobe using a 20X objective (Leica HCX APO L U-V-I 0.80 W). One z-stack was in bright field and acquired at 20 msec exposures ...
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bioRxiv - Plant Biology 2022Quote: ... high-light illumination of the cells was achieved by exposing the cuvette to white light set to an intensity of 620 μmol photons m-2 s-1 (Leica KL1500 LCD, peak 648 nm, FWHM 220 nm), chosen as the minimal light level necessary to saturate photosynthetic reactions and induce qE activity at the onset of illumination ...