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Citations for Leica :
1751 - 1800 of 1851 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and freeze substituted in anhydrous acetone containing 1% OsO4/2% H2O for 64 h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in EMbed 812 ...
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bioRxiv - Neuroscience 2024Quote: ... the hippocampi of 1-month-old male mice were dissected and sliced transversally using a vibratome (model VT 1200S; Leica Microsystems) with a thickness of 300 µm ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture was incubated at room temperature for 5 min and 1 μL of each sample was pipetted onto a glass dish and imaged using THUNDER Imaging Systems (Leica Microsystems).
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Bioengineering 2023Quote: ... one ending of the tube was connected to the tip of a syringe (cut from a 1 ml syringe; Braun, 9166017V) which held the perfusion needle (Leica, 39471024), and the other ending was immersed in the solution chamber where the animal was placed ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed onion epidermal cells were inoculated on 1/2MS agar plates for ∼12h in the dark and then visualized under a confocal microscope (Leica SP8) for YFP signals ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with 1 µM siR-DNA solution and subsequently migratory behavior of the cells was recorded under a confocal-microscope (Leica SP8) for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 4μm sections of organoid blocks were stained with ARID1A antibody (ab182560, 1/3000) on the automated Leica BOND RX system (Leica Biosystems).
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bioRxiv - Microbiology 2024Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Developmental Biology 2024Quote: ... The individuals were first anesthetized (with MS-222, at a concentration of 150 mgL−1) and then photographed under a stereoscopic microscope (Leica, Germany) to measure the TL ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Biophysics 2019Quote: Samples were freeze-substituted in 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica AFS (Leica Microsystems, Vienna, Austria) following a previously described protocol [52] ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were stained with phalloidin 647 and fluorescent z-stacks were acquired by confocal microscopy (Leica SP8, or Zeiss Examiner Z.1) at a pixel size of 60-70 nm ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...
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bioRxiv - Bioengineering 2020Quote: ... Observations of the samples were carried out under fluorescence microscope (Axio Imager D.1, Zeiss) and confocal microscope (Leica SP5 upright Confocal).
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Microbiology 2020Quote: ... Max projections of confocal z-stacks (25 z steps spaced 1 μm) were acquired on a SP5 resonant APD (Leica, Wetzlar, Germany) at 1.7x zoom using a 63x glycerol objective (numerical aperture 1.4).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Neuroscience 2021Quote: Every 4th brain section was imaged as a Z-stack (1 μm step size) using either the Zeiss LSM 800 (Zen, v2.6.76) or Leica SP8 confocal microscope (Leica Application Suite X, v3.5.6.21594) at 20x magnification ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were stored at −80°C for less than 1 week prior to sectioning (10 μm thick) using a CM1950 Cryostat (Leica, Wetzlar, Germany). Tissue sections were fixed and permeabilized in 1:1 methanol:acetone solution at −20°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were heated at 60°C for 1 h in an oven before H&E staining in the autostainer (Leica Autostainer XL). IHC was performed using a detection kit (Vector Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at -180°C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Plant Biology 2023Quote: 50 µm-thick cross sections were prepared from apical and basal internodes of Arabidopsis inflorescence stems at stage 1 (Altamura et al., 2001) by a vibrating blade microtome (Leica VT1200 S), stained with a 0.05% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was performed using Leica DMI8 confocal microscope and HC PL APO CS2 20x objective (excitation, 488 nm, emission, 498 nm - 550 nm, pinhole size of 1 Airy Unit) (Leica Microsystems, Germany), pixel dimensions 0.48 μm px-1 ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody incubation at 1:250 dilution for 30 minutes was followed by detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... the nuclei were stained with DAPI (1:2000) and the images were captured with 63X oil immersion objective by the confocal microscope (Leica STELLARIS 5). The measurements related to cell shape analysis of the nuclei in apical ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were let dry for at least 1 h prior to imaging at the microscope (Leica DM 2000 LED fitted with a Leica DFC450c camera). Ten images from different regions of the slide were acquired and the number of invaded cells counted using custom-made software further described in the next section ...
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bioRxiv - Plant Biology 2024Quote: ... Root tissue was then embedded in 5% low-melting point agarose (agarose dissolved in 1× PBS) and the embedded blocks were sectioned using a Leica RM2255 microtome (Leica, Nussloch, Germany). The root tissue sections were separated from agarose by rinsing ...
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bioRxiv - Molecular Biology 2023Quote: ... blotted with Whatman 1 filter paper and vitrified into liquid ethane at –180 °C using a Leica GP2 plunger (Leica microsystems, Austria). Frozen grids were transferred onto a Talos 200C Electron microscope (FEI ...