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Citations for Leica :
1501 - 1550 of 1673 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Neuroscience 2022Quote: ... 2-photon imaging was performed using an upright microscope optimized for confocal and multiphoton microscopy (TCS SP5 II, Leica Microsystems). After the mouse was mounted to a stereotaxic holder (SR-AM and STS-A ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... were imaged using a 0.75 NA 20X objective at 2 μm depth intervals on a Leica TCS SP8 laser scanning confocal microscope (Leica Microsystems). For the validation of the mouse line (assay # 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Non-consecutive sections were cut with a thickness of 10μm and placed onto 2 capture areas of 10X Visium Spatial Gene expression slide using a cryostat (Leica CM3050S). The tissue Optimization kit was used to determine permeabilization times (24 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were injected intravascularly in the duct of Cuvier with 27,6 nL of Membright Cy5-labeled tEVs or 4T1CD63pHluorinmScarlet-tEVs (at 10E10 EVs/ml) with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica) as described previously (Mary et al. ...
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bioRxiv - Developmental Biology 2024Quote: Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Cell Biology 2024Quote: ... or 2 hours of 200nM BafA1 before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were baked and dewaxed on Leica Bond-RX and pretreated with Bond Epitope Retrieval Solution 2 (ER2) (AR9640) from Leica at 100°C for 40 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... They were then embedded in 2% agarose and sliced into coronal sections (70-100µm thick) using a Vibratome (Leica Microsystems). The slices were washed in 0.01M PBS ...
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bioRxiv - Neuroscience 2024Quote: Confocal acquisitions were performed using a Leica SP8 White Light Laser 2 on an inverted stand (Leica Microsystems, Mannheim, Germany) and with a x20 (NA 0.75 ...
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bioRxiv - Neuroscience 2024Quote: ... frozen at -80°C for 30 minute then at -20°C for 2 hours before being sectioned using a cryostat (Leica, 50 µm thick coronal sections for immunocytochemistry) ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... embryos were introduced into an M9 droplet on a 2% agarose pad and immediately imaged on a Leica SP8 confocal on a DM6000 upright microscope (Leica LAS X image acquisition software ...
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bioRxiv - Immunology 2022Quote: ... was performed on sections (5 mm thick) of formalin-fixed lung mounted onto charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Neuroscience 2021Quote: ... were made from 5–7-week-old wild-type animals (C57Bl/6j, Janvier) using a vibrating blade microtome (VT1200, Leica Microsystems, Wetzlar, Germany) in NMDG based cutting solution (in mm) ...
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bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
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bioRxiv - Bioengineering 2022Quote: ... 3.033 μm and z: 5 μm) of the intervention hemisphere were acquired using a confocal laser scanning microscope SP8 (Leica Microsystems, Wetzlar, Germany) with a 10x objective ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Plant Biology 2021Quote: ... The root segments were embedded in agarose 5% (w/v) and cut into 25-30 μm radial sections with a VT1200S vibratome (Leica Microsystems, Nanterre, France). Radial sections were sampled from 15 ectomycorrhizal rootlets (at 200 µm from the root apex ...
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bioRxiv - Microbiology 2022Quote: ... tremulum culture was stained with Auramine O and examined by using the Leica TCS SP 5 Confocal Laser Scanning Microscope (Leica Microsystems, Wetzlar, Germany) in the Laboratory of Bioimaging facility NCCS ...
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bioRxiv - Cell Biology 2021Quote: The epithelium at ALI were incubated at 37°C and 5% CO2 in the 3i Marianis/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA) and high speed time-lapse videos were taken at 32X air at 100 Hz with a total of 250 frames using a scientific Hamamatsu C1140-42U30 CMOS camera (Hamamatsu Photonics ...
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bioRxiv - Cancer Biology 2021Quote: Sections with a thickness of 5 µm of lung adenocarcinoma and matched adjacent non-tumor lung tissue were mounted on MembraneSlides (Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin ...
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bioRxiv - Neuroscience 2020Quote: ... and the brain was cut into 400-um thick coronal sections using a vibratome (Leica VT1000S; Leica, speed 5, vibration frequency 7-8), while in bubbling ice cold ACSF (recipe as above ...
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bioRxiv - Pathology 2023Quote: ... The tissue samples were decalcified and embedded in paraffin before the preparation of coronal sections of 5-μm thickness around the ligation site using a microtome (RM2245, Leica Microsystems, Wetzlar, Germany). Tissue sections were then subjected to TRAP staining using an acid phosphatase leukocyte kit (Sigma-Aldrich ...
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bioRxiv - Zoology 2022Quote: ... and sectioned serially into 5 μm transverse or coronal tissue sections using the Leica X Multicut semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). Additional samples from juvenile T ...
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bioRxiv - Plant Biology 2022Quote: Sections of 5 or 10 μm were prepared on a Leica JUNG RM2055 microtome using a microtome knife (Leica Disposable blades TC-65). The sections were imaged without staining or after staining with Safranin O (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... H-1000) and visualized using a Leica Stellaris 5 Confocal Microscope equipped with a 40x oil-immersion objective (Leica Microsystems Inc, Illinois, USA). Fluorescence intensity profiles across sarcomeres were plotted offline using the FIJI software.
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bioRxiv - Evolutionary Biology 2023Quote: Rehydrated brains were embedded in agarose gel (5% UltraPure agarose power in deionised water) and sectioned using a vibrating blade microtome with an 80 µm interval (Leica VT1200, Wetzlar, Germany). Sections were then washed six times in PBS-TX (0.5% Triton X-100 detergent in phosphate-buffered saline) ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000; Leica Microsystems, Heidelberg, Germany) at high magnification (x200 or x400 depending on the immunolabeled density of cells) ...
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bioRxiv - Plant Biology 2024Quote: 3-D fluorescence imaging of plant tissues was acquired on a Leica STELLARIS 5 confocal microscopy (Laser lines: 488, 561, 638; Leica HyD photon detector).
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bioRxiv - Immunology 2024Quote: ... Serial 5-µm sections were stained with hematoxylin and eosin (H&E) and images were acquired using Aperio AT2 (Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2024Quote: Tilescans and z-stacks encompassing the entire VP were acquired using a confocal microscope (Leica Stellaris 5 LIA; Leica Microsystems, Deer Park, USA) under the 20X objective ...
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bioRxiv - Cell Biology 2024Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
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bioRxiv - Biophysics 2024Quote: ... and 7.5 μm thick cryosections were prepared on Superfrost™ microscope slides (Epredia, Kalamazoo, US) using a Leica CM3050 S cryomicrotome (Leica, Wetzlar, Germany) and stored at -80°C ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Then 3 μL of concentrated hTg were dispensed to the hydrophilic surface of the grid prior to single side blotting for 2 s and plunge freezing in liquid ethane using a Leica EM GP2 plunger (Leica Microsystems) operating at 20 °C and 80% relative humidity.
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bioRxiv - Evolutionary Biology 2022Quote: ... The fixed samples were embedded in Technovit 8100 (Kulzer, Germany) and processed into 2 μm tissue sections using a rotary microtome RM2255 (Leica, Germany). The AlexaFluor555-labeled oligonucleotide probes Eco934 (5’-CAT GCT CCA CCG CTT GTG-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Physiology 2021Quote: ... Images were acquired at a rate of 50 Hz with a wide-field fluorescence microscope (DMIRE-2, Leica Microsystems, Wetzlar, Germany) as previously described [24].
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bioRxiv - Immunology 2020Quote: ... The uptake of the fluorescently labeled SVV in IBRS-2 and 293T cells was visualized with a fluorescence microscope (Leica, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type 1 dpf larvae were positioned on top of a 2% agarose plate and imaged using a Leica M165FC dissection scope (Leica Microsystems) with a SPOT RT3 camera (SPOT Imaging) ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Bioengineering 2021Quote: ... the impact velocity was 6.0 m/s and the depth of cortical deformation was 2 mm with 100 ms impact duration (Impact ONE; Leica Microsystems). After the impact ...
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bioRxiv - Plant Biology 2020Quote: ... mesophyll cells of 2-week-old transgenic Arabidopsis plants expressing Raf36-GFP were observed with a confocal microscope SP8X (Leica Microsystems) with the time-gating method (Kodama ...