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Citations for Leica :
1451 - 1500 of 1673 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... H&E staining was performed on 5 µm sections from paraffin-embedded retinas according to standard protocols for light microscopy and photomicrography (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Cells were then imaged at 37°C in a chamber supplemented with humidified 5% CO2 through white light laser confocal microscope TCS SP8 (Leica, Wetzlar, Germany). Movie S1 was processed by Bitplane-Imaris 9.5 (Andor Technology ...
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bioRxiv - Biophysics 2020Quote: ... stage of growth were imaged every 10 minutes for 12 hours in a customized incubator (37°C, 5% CO2, and 95% humidity) on a confocal microscope (Leica, TCL SP8), with acquisition of fluorescent and bright-field image z-stacks (Fig ...
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bioRxiv - Bioengineering 2021Quote: ... The samples washed in PBS (3x 5 min) and embedded in Mowiol prior to confocal laser scanning microscope analysis (Leica TCS SP8X). Collagen fiber organization was determined as described in the supplementary information (supplementary Fig.S2-4 and supplementary Eq.S1.).
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bioRxiv - Immunology 2021Quote: ... Lung tissue was embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and 5 μm sections were cut on CM1950 Cyrostat (Leica, Weltzlar, Germany). Sections were blocked with 1% Rat serum and 1% FcBlock in PBS for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were collected onto cover glasses and coated with gold with sputter current 30 mA for 5 seconds (Leica EM ACE200). Cover glasses were sealed on aluminum stabs using carbon tape ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm each of carbon and iridium (Leica ACE 600). Prepared samples were imaged on a Zeiss Merlin FE-SEM using an InLens detector at 1.2 – 1.5 kV and 400 – 500 pA depending on the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems, Nussloch, Germany). Brain sections were labelled with Anti-FLAG M2 F1804 ...
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bioRxiv - Plant Biology 2023Quote: ... Stems were rehydrated first in ethanol 50% and later in water and then embedded in agarose 5% before being transversally cut using a vibratome (Leica vt 1000s) to obtain 120 micron thickness sections stored in water ...
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bioRxiv - Neuroscience 2022Quote: ... Delineation of CA1.SR and MDG subregions were performed using light microscope under 5 × objective lens, the newCAST software (Visiopharm, Hørsholm, Denmark) modified for stereology with a digital camera (Leica DFC 295, Germany) and a motorized stage (Ludl Mac 5000 ...
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bioRxiv - Plant Biology 2022Quote: ... The abaxial side of the leaf sample was imaged using a 63× objective (Leica, HC PL APO CS2 5 63×/1.20 Water). GFP was excited with a 488 nm WLL laser and collected at 503-530 nm ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Developmental Biology 2023Quote: The images were initially processed in Fiji with a customed macro to batch open the lif files (Image taken on Leica Stellaris 5) and split the images into individual channels ...
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bioRxiv - Microbiology 2022Quote: ... cells were imaged using a Stedycon optical setup (Abberior Instruments GmbH) integrated with Leica SP 5 microscope (Leica Microsystems GmbH, Manheim, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Fixed brains were washed three times for 5 min with PB and 150 µm-thick coronal sections were cut with a vibratome (Leica Microsystems, VT1000S). Tissue sections were labeled with DiIC18 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal and para-sagittal slices (300 μm) were prepared in ice-cold oxygenated (95% O2 / 5% CO2) ACSF with a vibratome (LeicaVT1000S, Leica Biosystems, Germany), then warmed to 36°C for 45 mins ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
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bioRxiv - Biophysics 2024Quote: ... alveolospheres were cultured in 35 mm petri dish in a customized incubator (5% CO2, 37°C, 95% humidity) on a confocal microscope (Leica, TCL SP8). A 25 × /0.95 numerical aperture water objective and a 63×/1.20 numerical aperture water objective were both used for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... 2.4 CaCl2, and 10 D-glucose bubbled with carbogen gas (95% O2, 5% CO2) using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were allowed to recover for at least 30 min while submerged in a chamber filled with oxygenated aCSF at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Z-stack images with a spacing of 5 µm were obtained using an epifluorescence microscope with an automatic scanning stage (DMi8 S; Leica, Wetzlar, Germany) equipped with a ×10 dry objective (Leica ...
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bioRxiv - Biophysics 2020Quote: ... S cerevisiae (Sc) cells strain #2 after ~5h of starvation were high pressure frozen (HPM010, AbraFluid) and freeze substituted (EM-AFS2, Leica) with 0.1% uranyl acetate in acetone for 55h at −90°C ...
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bioRxiv - Biochemistry 2022Quote: ... Neutrophils were then counted at the site of transection at 2 hours post injury (hpi) using a fluorescence dissecting stereomicroscope (Leica).
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bioRxiv - Developmental Biology 2021Quote: ... Immunofluorescent analysis and imaging were performed on a Leica SP5X Upright 2-Photon Confocal microscope using LAS AF software (Leica) and a Leica 63X (type ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Plant Biology 2021Quote: ... The cross section of 2-4μm was cut with a glass knife using the ultramicrotome Leica EM UC7 (Leica Microsystems). The semi-thin sections were stained for 30mins in 0.1% aniline blue in 0.1M K3PO4 at RT ...
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bioRxiv - Plant Biology 2021Quote: ... The stained sections were observed within 1hr to detect callose deposition using confocal laser scanning microscopy (TCS SP5 2; Leica).
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bioRxiv - Neuroscience 2022Quote: ... brains were flash frozen in 2-methylbutane and stored at −20°C until sectioning at 40μm on a cryostat (Leica Biosystems) into cryoprotectant.
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bioRxiv - Immunology 2022Quote: Staining for SARS-CoV-2 antigen was achieved on the Bond RX automated system with the Polymer Define Detection System (Leica) used per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI) fluorescence signals were detected by confocal laser-scanning microscopy analysis using TCS SP8 (Leica Microsystems). All the images were obtained and processed with Leica LAS AF Lite software ...
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bioRxiv - Physiology 2021Quote: ... snap-frozen in 2-methylbutane on dry ice and sectioned into 10 μm slices using CryoJane Tape-Transfer System (Leica). Masson’s trichrome stain for cardiac fibrosis was performed according to the manufacturer’s instructions (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Thin sections from the area of 2-photon imaging were cut with a diamond knife on an EM UC6 ultramicrotome (Leica), collected on pioloform-coated copper Synaptek slot grids (Electron Microscopy Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 2 µL of the spore suspension per sample was then processed with Leica EM ICE HPF apparatus (Leica Microsystems), and then freeze substituted and embedded in Lowicryl HM20 (Electron Microscopy Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... We cut the Spurr’s blocks into semi-thin sections (0.75–2 μm) with glass knives on an ultramicrotome Leica Ultracut UCT (Leica Microsystems) stained with a solution containing 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Images were captured using Metamorph software at every 15 minutes in 37C (PeCon Tempcontrol 37-2) using confocal spinning disk system (Leica DMI 6000 B equipped with YOKOGAWA CSU-W1 Confocal Scanner Unit ...
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bioRxiv - Neuroscience 2021Quote: ... embedded in 2% LTE agarose/ Tris-buffered saline (TBS) and cut coronally in 50 µm sections on a vibratome (VT 1000S, Leica). Sections underwent antigen retrieval in a solution of 0.1 M sodium citrate (pH 6) ...
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bioRxiv - Developmental Biology 2021Quote: ... The tissues were then cryoprotected in 10% and 30% sucrose solutions for 2 hrs at RT and finally incubated in 30% sucrose and tissue embedding medium (Leica) (1:1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 25% laser power (Pnmy-2nmy-2::gfp) or 13% laser power (Ppie-1mCherry::phPLCδ) was used (Leica TCS SP5-II). All analyses were performed on center Z-slices of Z-stacks through the NSMnb ...
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bioRxiv - Neuroscience 2023Quote: ... brains tissue fixed in 4% paraformaldehyde were cryoprotected in 30% sucrose for 2 hours followed by embedding in OCT for sectioning to 10μm thickness on a cryostat (Leica CM1860). Prior to staining ...
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bioRxiv - Genetics 2023Quote: ... the worms were mounted on a 2% agarose pad and images were captured using a Leica compound microscope (Leica DM5000B).
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bioRxiv - Immunology 2023Quote: ... as described previously.23 All heat induced epitope retrievals were performed with Bond™ Epitope Retrieval 2 (AR9640, Leica Biosystems) for 20 minutes at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... the brain was cut into 2 mm thick coronal sections in 0.15 M cacodylate buffer using a vibratome (Leica VT1200). Then ...
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bioRxiv - Bioengineering 2022Quote: ... a ~ 2 nm layer of gold coating was completed using a sputtering machine (Leica EM ACE 600, Buffalo Grove, IL) and was imaged using an SEM (Apreo 2 ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Neuroscience 2023Quote: ... five µm section were cut followed by a Heat-Induced Epitope Retrieval at 100°C during 20 min in BOND Epitope Retrieval 2 (ER2) solution (Leica) with 0.1 Proteinase K before Deparaffinizing ...