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Citations for Leica :
1301 - 1350 of 1667 citations for RGPD1 2 3 4 5 8 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000 ...
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bioRxiv - Bioengineering 2022Quote: PTFE surfaces were sputter-coated with platinum to develop a 5 nm thick conductive layer on the surface using an EM ACE600 sputter coater (Leica). Images were acquired using a Quanta 450 scanning electron microscope (FEI company).
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bioRxiv - Biochemistry 2022Quote: ... serial 5 μm thick sections of adult scallop’s eye were obtained using a Leica CM1950 freezing microtome (Leica, Wetzlar, Germany) for Raman spectroscopy testing (alpha300R ...
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bioRxiv - Immunology 2023Quote: ... with a stage top incubator to maintain 37 °C and 5% CO2 or on a Leica TCS SP8 Laser Scanning Confocal (Leica) fluorescence microscope with an incubator to maintain 37 °C and 5% CO2.
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bioRxiv - Biophysics 2023Quote: ... A volume of 3ul of the sample was applied onto glow-discharged (Leica EM ACE200, 30 s at 5 mA) grids (Quantifoil R1.2/1.3 200 mesh Cu grids ...
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bioRxiv - Developmental Biology 2023Quote: ... and 18.5 counting images were acquired using Leica Application Suite X (LAS X) on a Leica DMI8 fluorescent microscope from Leica Microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
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bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were imaged either with a Zeiss Observer A1 microscope and the AxioVision software 4.8 or with a STELLARIS 5 confocal microscope (Leica, Germany) at 40x (HC PL APO 40x/1.30 OIL CS2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 μm or 0.5 μm step sizes was performed on a Leica TCS sp8 Lightning Confocal/STED microscope coupled to LasX software (Leica) using a 63x (1.4 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells expressing OMM-Cepia3 and ER-mit RspA-splitFAST were mounted into an open-topped chamber in mKRB supplemented with TFCoral (5 µM) and imaged by a Thunder Imager 3D Cell Culture (Leica), equipped with a LED8 illumination system ...
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bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
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bioRxiv - Plant Biology 2023Quote: ... cell walls were labelled with propidium iodide (5 µg/mL) and imaged using a 20x PLAN APO multi-immersion objective (Leica), with λexc = 488 nm and λem = 551-651 nm ...
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bioRxiv - Pathology 2023Quote: ... 5 μm thick sections were taken from the paraffin blocks using a rotary microtome (Leica RM2155, Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin-eosin (HE) ...
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bioRxiv - Plant Biology 2024Quote: ... The cells were then washed 3 times for 5 min with PBS and covered with PBS before imaging them with a TCS SP8 confocal laser-scanning microscope (Leica). Excitation/emission settings were 488 nm/498-524 nm HyD1 SMD hybrid detector for CAH4 ...
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bioRxiv - Developmental Biology 2023Quote: ... ganglionic eminences explant cultures were placed in a humidified and thermoregulated chamber maintained at 37 °C and 5% CO2 on the stage of a spinning-disk microscope (Leica), equipped with an Orca Flash 4.0 Camera ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin-embedded tissue was sectioned into approximate 0.5cm3 cubes and then cut into 5-μm slices by a LEICARM2235 Slice Machine (Leica, Germany). The samples were stained with haematoxylin/eosin (H/E ...
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bioRxiv - Neuroscience 2024Quote: ... 87 mM NaCl and 25 mM D-glucose) gassed with 95% O2/5% CO2 to give a pH of 7.4 using the VT-1200 Vibratome (Leica, Germany). Slices were then allowed to recover for at least an hour in a chamber filled with ACSF ...
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bioRxiv - Biophysics 2024Quote: ... were evenly positioned on a ring installed 5 cm above the sample positioned at the focal plane of the objective lens (OBJ; 100×/1.44, Leica, Germany), as shown in Fig ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
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bioRxiv - Neuroscience 2019Quote: Wide-field calcium imaging of Hydra was conducted at 2 Hz using a fluorescence dissecting microscope (Leica M165) equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC) ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
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bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Immunology 2022Quote: ... formalin-fixed paraffin-embedded tissue sections were pre-treated with the BOND Epitope Retrieval Solution 2 (Leica Biosystem) at 100°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... 2’,7’-dichlorofluorescein (DCF) fluorescence was visualized by confocal laser scanning microscopy (CLSM) (Leica TCS-SP5, Mannheim, Germany) with excitation/emission at 495 nm/525 nm.
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bioRxiv - Microbiology 2020Quote: ... 0.05 % uranyl acetate in acetone and placed in an AFS-2 freeze-substitution machine (Leica Microsystems, Wetzlar, Germany). Tissues were freeze-substituted at −90 °C for 72 h ...
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bioRxiv - Pathology 2021Quote: ... These were double contrasted using aqueous 2% uranyl acetate (Honeywell International Inc.; Morristown) and lead citrate solutions (Leica) for 10 min each ...
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Multiplexed single-cell transcriptomic analysis of normal and impaired lung development in the mousebioRxiv - Cell Biology 2019Quote: ... Retrieval was accomplished using an ethylenediaminetetraacetic acid (EDTA) buffer (Bond epitope retrieval solution 2, Leica, Concord, ON, Canada), pH9 for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... in dry methanol for 32 hours at −90° C in an AFS 2 freeze substitution unit (Leica Biosystems). The temperature was then raised 5° C per hour to −45° C ...
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bioRxiv - Microbiology 2020Quote: ... samples were de-stained with 50% Lactophenol for 1–2 hours before observation under a light microscope (Leica Microscope ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265 ...
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bioRxiv - Microbiology 2023Quote: ... was applied to glow-discharged Quantifoil R2/2 grids and vitrified with a GP2 plunge-freezing device (Leica).
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bioRxiv - Plant Biology 2023Quote: Roots fixed with 2% (v/v) formaldehyde in Tris were embedded in Cryo-Gel (Leica Biosystems, ID:39475237) and cut onto a cryostat (Leica CM1950 ...
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bioRxiv - Biophysics 2023Quote: ... FFPE tissue samples were cut using a ultramicrotome in 2 πm thick sections (RM2125 RTS, Leica Biosystems, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Physiology 2024Quote: ... and Mac-2-staining were imaged using a Leica DMi8 Inverted Fluorescent Microscope (Leica Microsystems, Buffalo Grove, IL). Histological quantifications were performed using Image J software (v1.52q ...
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixated overnight in 4% paraformaldehyde, followed by sectioning (coronal plane, 50 μm) with a vibratome (VT100S, Leica Biosystems, Wetzlar, Germany). The slices were collected and stored in PB for immunohistochemistry.
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bioRxiv - Immunology 2019Quote: ... eight histological sections from central region of the each alveolar socket were stained with Picrosirius Red stain and the images of 4 histological fields a 40× objective were captured by a polarizing lens coupled to a binocular inverted microscope (Leica DM IRB/E), as previously described [55] ...
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.