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Citations for Leica :
1251 - 1300 of 1667 citations for RGPD1 2 3 4 5 8 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were post-fixed overnight in 4% paraformaldehyde and then cryoprotected in a 30% sucrose and 0.1M PBS solution prior to sectioning (Leica CM3050S, Leica Biosystems). Three sets of immediately adjacent sections (40 μm ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... A series of 4-μm thick longitudinal sections and transverse sections (rapidly elongating region) were made with a rotary microtome RM2265 (LEICA EM UC7). Fixed sections were stained with toluidine blue O for 30 min and photographed with an Olympus BX51 microscope equipped with a DP74 camera.
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bioRxiv - Neuroscience 2019Quote: ... Brains were postfixed in 4% paraformaldehyde overnight on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S), with sections collected into six tubes (containing cryoprotectant solution as described[32] ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... The embedded tissue blocks were serially cut into 5-μm-thick ground sections using a microtome (Leica SM 2000; Germany) and mounted on glass slides ...
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bioRxiv - Neuroscience 2019Quote: ... Imaging was performed for 5min at 1Hz at 37°C 5% CO2 on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40 ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were placed on the ice block to dry for 5 minutes prior to LCM using a laser microdissection microscope (Leica).
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bioRxiv - Developmental Biology 2021Quote: ... Stained embryos were embedded in Technovit 7100 resin (Kulzer Technique) and 5 µm sections were obtained using an RM2245 microtome (Leica). Sections were counterstained with Mayer’s hematoxylin solution and images were captured by BX61 microscope (Olympus ...
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bioRxiv - Biophysics 2020Quote: ... These polyps were then embedded in 5 % Agar and sectioned into 200 μm slices using a vibratome (Leica VT1200 S). The sections were then stained with 1 % Osmium tetroxide (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting samples were cut to 5 µm by using a Reichert-Jung 820H Histostat rotary microtome (Leica, Wetzlar, Germany) and Drukker VC186200 diamond knives (Cuijk ...
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bioRxiv - Neuroscience 2020Quote: ... brains were embedded in a 5% low-gelling agarose gel (in PBS) and 50µm coronal slices were cut using a vibroslicer (Leica VT1000S). Slices were then stored in PBS-Azide (0.02-0.05% ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA (10 µl of 5 µg/µl solution) was injected into each testis using glass capillaries under a stereomicroscope (M165C; Leica). Testes were held between a pair of tweezers-type electrodes (CUY21 ...
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bioRxiv - Neuroscience 2022Quote: ... Near-horizontal middle entorhinal cortical (bregma –6.5 mm to –5.1 mm) slices of thickness of 350 μm were obtained using a vibrating blade microtome (Leica Vibratome) while the brain was submerged in oxygenated ice-cold cutting solution ...
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bioRxiv - Cell Biology 2019Quote: ... Tissue was rinsed in MilliQ water for 5 minutes and then incubated in Hematoxylin 560 MX (Leica Biosystems, cat. 3801575) for 2 minutes and in running water for 1 minute ...
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bioRxiv - Biochemistry 2019Quote: 5-micron FFPE sections were mounted onto MMI membrane slides (MMI, 50102) and stained using an automated stainer (Leica XL) at the Histology Core at University of Manchester ...
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bioRxiv - Neuroscience 2019Quote: ... Rorb thickness was quantified by dividing the cortical plate into 5 equal bins and intensity in each bin was quantified using Aperio ImageScope (Leica).
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bioRxiv - Plant Biology 2019Quote: ... cross sections 5 µm thick were obtained with an automatic advanced rotary microtome (model RM2155, Leica microsystems Inc., Deerfield, USA), were stained with toluidine blue then photographed using a digital camera (Zeiss AxioCam HRc ...
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bioRxiv - Plant Biology 2019Quote: ... Semi-thin sections (5 μm thickness) for light microscopy were obtained with a microtome (HM 335E, Leica Microsystems, Heerbrugg, Switzerland) and stained with toluidine blue O.
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bioRxiv - Microbiology 2021Quote: ... imaging the colony and crystal formation at regular intervals at 5× magnification with a Leica MZ7.5 stereomicroscope (Leica, United Kingdom), using an Infinity 2 Lumenera microscope camera (Teledyne Technologies Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μm sections were taken for H&E staining using standard methods and imaged using a DMI 6000B microscope (Leica). To examine tdTomato expression in sections ...
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bioRxiv - Cancer Biology 2022Quote: Hematoxylin and Eosin (H&E) staining was performed on 5 μm thick slices using an automated system (Leica Autostainer, ST5010) with the protocol as described in Supplemental Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Retinal cups were embedded in 5% agar moulds and immediately underwent sectioning on a fully automated vibratome (Leica VT1200 S). Sections were ‘Chased’ by incubation with 0.6 µM SNAP-Cell 647-SiR (New England Biolabs (S9102S ...
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bioRxiv - Neuroscience 2022Quote: ... Horizontal slices containing the substantia nigra were cut at 222 μm in Krebs buffer bubbled with 95/5% O2/CO2 using a vibrating microtome (Leica). Slices were allowed to recover at 30°C in vials with 95/5% O2/CO2 Krebs with MK801 (10 μM ...