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Citations for Leica :
1251 - 1300 of 1348 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Paraffin-embedded WAT was sliced into 5 μm sections using a Thermo Scientific™ HM 355S Automatic Microtome and mounted on Surgipath® X-tra® Microscope Slides (Leica Biosystems). To represent the whole tissue ...
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bioRxiv - Microbiology 2022Quote: ... The target probe was validated on reproductive tracts from mice experimentally inoculated with Chlamydia muridarum strain Nigg.5 Slides were stained on an automated stainer (Leica Bond RX, Leica Biosystems) with RNAscope 2.5 LS Assay Reagent Kit-Red (322150 ...
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bioRxiv - Neuroscience 2020Quote: ... equilibrated with 95 % O2-5% CO2) and parasagittal spinal cord slices (280 μm) were prepared with a vibratome (VT1200S, Leica Microsystems AG, Wetzlar, Germany) in the same solution ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in ice-cold 95% O2/5% CO2-equilibrated sACSF and sectioned at 250 µm in the sagittal plane with a vibratome (VT1200S; Leica Microsystems Inc., IL, USA). Slices were then transferred to a holding chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... IHC staining for CAV1 was performed on formalin-fixed paraffin-embedded HER2-expressing gastric tumor sections (5 μm thickness) on a Leica Bond RX automated stainer (Leica Biosystems, Buffalo Grove, IL). Following deparaffinization and heat-induced epitope retrieval in a citrate buffer at pH 6.0 ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anaesthetized using 2.5% isoflurane and 1.5 l/min air for 5 min and impacted directly over their intact head using an electromagnetic impactor (Leica Impact One Stereotaxic impactor, Leica Biosystems, Richmond, IL, USA) with a 3mm round tip at a speed of 3m/s ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then mounted on aluminum stubs with carbon adhesive tabs and coated with 5 nm of carbon and 6 nm of iridium (Leica ACE 600, Vienna, Austria). SEM images were acquired on an FE-SEM (Zeiss Merlin ...
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Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cuesbioRxiv - Developmental Biology 2023Quote: ... The images were captured by the Leica STELLARIS 5 confocal microscope with 63X oil immersion objective ((Leica; HC PL APO 63x/1.40 OIL CS2) using Application suite X.
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bioRxiv - Plant Biology 2022Quote: To generate the training set we used two different stereo microscopes equipped with an integrated camera for image capture (ZEISS Stemi 305 with a 1.2 Megapixel integrated colour camera; Leica MZ16 coupled with a Leica CLS100x white light source and a Leica DFC420 5 Megapixel colour camera). For the downstream experiments using the adapted CNNs we only used the ZEISS Stemi 305 since it is easier to operate and to transport to the field ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The number of fluorescent and non-fluorescent worms was counted in triplicates in droplets of 5-20 µl depending on worm density using a fluorescent dissecting scope (Leica Microsystems GmbH, Wetzlar, Germany). Competitive fitness was determined as relative proportion of non-fluorescent mutant worms compared to the fluorescent reference strain CL2122.
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bioRxiv - Bioengineering 2024Quote: ... and the cells were imaged daily for the next 5 days with an inverted microscope (Leica DM IL LED, Leica Microsystems, Buffalo Grove, IL) connected to a camera (Leica DFC3000G ...
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bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was blocked in 2% agar and 200 µm thick coronal sections were cut using a vibratome (Leica VT 1000 S). The slices were collected on gelatin-coated glass slides and stained with solution D and E before being dehydrated through graded alcohols and mounted using Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... GFP fluorescence resulting from LanB2:GFP expression in Dilp2 neurons was imaged with a Leica TCS SP8 MP 2-photon microscope (Leica, Wetzlar, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Brains were isolated and acute horizontal slices (310 μm thick) were prepared using a vibratome (5000mz-2, Campden Instruments Ltd.; or VT1200S, Leica Biosystems). Slices recovered for 30 min in ∼37°C oxygenated Ringer’s solution that was identical to the dissection solution except with lower Mg2+ concentrations (1 mM MgSO4 ...
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bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Histological analysis of the 2 µm paraffin-embedded tissue sections was performed with the Bond Polymer Refine Detection Kit (Leica, Wetzlar, Germany) on a BondMax system (Leica) ...
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bioRxiv - Plant Biology 2020Quote: ... Light scattering changes in the presence of 50 μM MV were induced by applying red light (1,000 μmol m−2 s−1, Leica KL 1500 LED). Scattering changes were measured first in dark for 100 s ...
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bioRxiv - Neuroscience 2021Quote: ... the cochlea was glued on a metal block with Loctite 401 and cut to 2 halves by a vibratome (VT1200S, Leica, Wetzlar, Germany) with FREQ index at 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 3-5 week old mice were sacrificed by Ketamine/Xylacine injection and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 220 μm sections using a slicing solution continuously oxygenated containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... in PBS and the extracted lumbar spinal cord portion was embedded in 2% low meting point agarose (Bio&SELL) and sliced with a vibratome (Leica VT1200S, Germany) into 300 μm sections using a slicing solution containing (in mM) ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of the samples were dispensed onto glow discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10°C ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Biochemistry 2023Quote: 3 µl of the individual complexes were applied onto glow-discharged Quantifoil holey carbon grids (Cu R2/1 or R2/2) and vitrified in liquid ethane (−181 °C) using a Leica GP plunger (Leica Microsystems, Austria) maintained at 90% humidity and 10 °C ...
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bioRxiv - Physiology 2023Quote: ... the worms were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images acquired using a confocal microscope (Leica TCS SPE) with a 63× objective lens ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal brain sections (20 μm) were cut around the bregma 0 to -2 mm using a Leica CM1950 cryostat (Leica Biosystems, Germany). For P2X7R immunofluorescence labelling ...
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bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the neuron somata were taken with 0.38 μm step size using a 63× oil objective with 2× digital zoom magnification (Leica TCS SPE, Germany). Stacks were then processed using FIJI/ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: ... Images from the same area of the CA1 region of each hippocampus were acquired using a Leica DMLB 2 microscope (Leica, Wetzlar, Germany) connected to a Leica DFC320 FireWire digital microscope camera (Leica ...
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bioRxiv - Bioengineering 2022Quote: Following embedding within 2% agarose and fixation overnight samples were dehydrated and embedded in paraffin using an automatic tissue processor (ASP300; Leica, London, UK). All samples were sectioned at a thickness of 6 μm using a rotary microtome (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Bioengineering 2023Quote: ... Lateral and longitudinal cross-sections of the samples were imaged after 7 days of culture in EGM-2 BulletKit medium using a Thunder imaging system (Leica Microsystems, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... the brains were removed and sectioned into 150 µm transverse slices after embedding in 2% low-melting agarose using a vibratome VT1000 (Leica, Wetzlar, Germany). The OTCs were cultured on Millicell cell cultures inserts in 6-well plates using 1 mL of FCS-free medium consisting of DMEM/F12 supplied with 1 x B27 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut, Leica Microsystems, Bensheim, Germany). The ultra-thin sections were post-contrasted in a LEICA EM STAIN (Leica Microsystems ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+-fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software for examining Ca2+ fluctuation amplitude and frequency ...
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bioRxiv - Neuroscience 2024Quote: ... Selected sections were also studied on a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) or scanned with an Olympus VS200 Research Slide Scanner (Olympus / Evident ...
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bioRxiv - Plant Biology 2024Quote: ... The plants were kept at 22℃ and leaves were screened after 2 to 3 days using a confocal microscope (Leica SP8 upright).
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and cut into coronal slices (thickness 300 µm, -1.5 mm to 2 mm relative to bregma) on a vibratome (Leica VT1200S, Leica Biosystems, Nussloch, Germany) in ice-cooled artificial cerebrospinal fluid (aCSF) ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were then embedded in 2 % agar in PBS and cut into sagittal sections with a thickness of 50 µm using a microslicer (VT1000S, Leica, Wetzlar, Germany). The serial sections were cover-slipped with mounting medium (DAPI Fluoromount-G ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections (∼2 x 2 x 2cm) not needed for pathologic evaluation were fixed in 10% formalin and sliced with a vibratome (Leica VT 1200S) to a thickness of 2mm ...
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2022Quote: ... The z-stack images of the whole tissue were acquired with a 5 mm step size in a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Images were segmented and volumetrically reconstructed with Imaris software (Bitplane ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Physiology 2021Quote: ... The lung tissue sections of 3-5 micron were stained with H&E stain and were microscopically analyzed using microscope (Make: Leica DM1000 LED; Camera: Leica MC170HD). The images were taken with 10X objective lens (Magnification X 100 ...