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Citations for Leica :
951 - 1000 of 1348 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Physiology 2024Quote: ... and Mac-2-staining were imaged using a Leica DMi8 Inverted Fluorescent Microscope (Leica Microsystems, Buffalo Grove, IL). Histological quantifications were performed using Image J software (v1.52q ...
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bioRxiv - Cancer Biology 2024Quote: ... heat-treated in ER2 (epitope retrieval solution 2) antigen retrieval buffer for 20 min at 93 °C (Leica), blocked in antibody (Ab ...
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bioRxiv - Cancer Biology 2024Quote: ... with a tissue pretreatment of 15 minutes at 95°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Microbiology 2022Quote: ... with a tissue pretreatment of 15 minutes at 90°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265 ...
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bioRxiv - Microbiology 2024Quote: ... with a tissue pretreatment of 30 minutes at 100°C with Bond Epitope Retrieval Solution 2 (Leica Biosystems) and 1:750 dilution of OPAL 570 (AKOYA ...
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bioRxiv - Neuroscience 2024Quote: ... Horizontal slices (300µm) of the OB were cut using a vibratome (Campden Instruments 7000SMZ-2 or Leica VT1000S) and stored in ACSF containing the following (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixated overnight in 4% paraformaldehyde, followed by sectioning (coronal plane, 50 μm) with a vibratome (VT100S, Leica Biosystems, Wetzlar, Germany). The slices were collected and stored in PB for immunohistochemistry.
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Pathology 2022Quote: ... in situ hybridization was performed on 12 µm-thick sections of 4% PFA-fixed OCT mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval (HIER ...
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bioRxiv - Bioengineering 2022Quote: ... Perfusion devices were then fixed with 4% para-formaldehyde and imaged using a 16x immersion lens on SP8 multiphoton microscope (Leica Microsystems, Morrisville, NC). Tumor volumes were quantified by imaging individual tumors post bioprinting
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then embedded in a 4% agarose/1x PBS w/v solution prior to mounting and slicing on a vibratome (Leica VT 1000 S). 40 μm or 60 μm sections containing the pPVN (−0.70 to −1.06 mm relative to Bregma) ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Physiology 2021Quote: ... Fluorescence emission was detected every 526 ms using a Leica SP8 DIVE multiphoton confocal fluorescence imaging system as described above (emission at 460-520 nm for Fluo-4 and at 580-640 nm for Fura Red) (Leica Microsystems, Heidelberg, Germany).
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Immunology 2023Quote: ... Formalin-fixed paraffin-embedded (FFPE) sections of 4 μm thickness were stained with antibodies specific for CD21 (predilute, clone 2G9, Leica Biosystems, Danvers, MA), CD3 (predilute ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X). WT ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X).
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
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bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were stored at 4°C and imaged using the 63x oil immersion objective on the Leica TCS SP5 confocal microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were sputter-coated with a 4 nm platinum layer using a Leica EM ACE600 sputter coater (Leica Microsystems GmbH, Wetzlar, Germany) and examined using a Zeiss Crossbeam 340 Field-Emission Electron Microscope (Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunohistochemistry protocol is briefly described as follows: tissue sections (4 μm) were stained in a Leica BOND RX automated stainer (Leica Biosystems Nussloch GmbH). The tissue sections were deparaffinized and rehydrated following the Leica BOND protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The surface of the cocoon and all the fibers were observed using scanning electron microscopy (SEM, Hitachi, Japan) at an acceleration voltage of 5 kV and a polarizing optical microscope (Leica, Germany). For transmission electron microscope (TEM ...
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bioRxiv - Developmental Biology 2021Quote: ... The entire cerebellum of each animal (N=5 analyzed) was sectioned coronally at 50μm thickness on a vibratome (Leica VT 1000S) and all sections were processed for immunohistochemistry using a human nuclear antigen antibody (HuNu ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Neuroscience 2020Quote: CGEs from E14.5 Plxna4+/+;Htr3a-GFP or PlxnA4-/-;Htr3a-GFP coronal acute brain slices were microdissected under a fluorescence dissecting scope (Leica M165FC) and cells dissociated with 0.25% trypsin-EDTA (Life Technologies) ...
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bioRxiv - Bioengineering 2020Quote: ... The diffusion experiments were performed in the environmentally controlled chamber (set at 37 °C, 5% CO2) of a fluorescent microscope (Leica, CTR6500). The dextran solution was perfused inside the microvessel channel and images were acquired every 30 sec over 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were rapidly removed from the skull and coronal brain slices containing the hippocampal formation (350μm thick) were prepared in the ice-cold cutting solution bubbled with 95%O2/5% CO2 using Vibratome VT1200S (Leica Microsystems) and then incubated in an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Genomics 2021Quote: ... Serial sections cut to a thickness of 5 μm were obtained using a microtome (Leica RM2125RT, Leica Microsystems Nussloch GmbH, Germany) and stained in Gill 2 hematoxylin (Thermo Scientific Kalamazoo ...
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bioRxiv - Microbiology 2021Quote: ... collecting ∼200 000 bright-field images at 5 ms resolution with a 100× oil objective on a Leica DMi8 inverted microscope (Leica, Germany) at a set temperature value of 30°C.
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bioRxiv - Microbiology 2021Quote: ... Images of the colony patterns were taken under a 5× objective using a Leica DM6000B fluorescence microscope (Leica Corporation, Wetzlar, Germany) equipped with a LED fluorescence illuminator (Leica Corporation) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were placed in an incubator chamber at 37 °C with 5% CO2 supplementation mounted on a Leica TCS SP8X microscope system (Leica Microsystems). Laser micro–irradiation was performed using a protocol adapted from (6) ...
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bioRxiv - Cell Biology 2021Quote: ... and xz images were acquired in the presence of the membrane dye CellMask (5 mg/ml, Life Technology) using Leica TCS SP5X confocal microscope (Leica Microsystems). The MFI of Fluo-4 in the cytoplasmic region in individual cells was measured using the NIH ImageJ software ...
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bioRxiv - Molecular Biology 2022Quote: ... The glycolytic and oxidative portions of the TA muscles of 5 mice in the CTR group and 5 mice in the Crush - 3 days group were microdissected with a laser microdissection system (Leica LMD6) and recovered in RNAlater reagent (QIAGEN).