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Citations for Leica :
1151 - 1200 of 1306 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... samples on SEM stubs were coated with a 10 or 15 nm platinum or gold layer using the sputter coating machine EM ACE600 (Leica Microsystems) at 30 mA current to be finally placed into the Crossbeam 540 FIB-SEM (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... H&E staining on 10 μM frozen section was performed using the Leica ST5020 Multistainer workstation (Leica Biosystems, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Four images of each well were taken with Leica DMi8 fluorescent microscope equipped with a 10× magnification objective (Leica, Wetzlar, Germany). Overall ...
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bioRxiv - Cancer Biology 2023Quote: ... A microarray of fresh-frozen DMBA/TPA induced squamous cell carcinoma samples was cryo-sectioned with 10 μm thickness (Leica, CM3050S) and placed on the capture area of a spatial gene expression slide (10× Genomics ...
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bioRxiv - Microbiology 2023Quote: ... sectioned them at 10 µm thickness using a microtome and mounted the sections on thermoexitable POL-membranes (No. 115005191; Leica, Germany). Next ...
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bioRxiv - Neuroscience 2023Quote: ... using the same acquisition setting for all slices and at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany). The images were analysed by a person blinded to the genotype using Qupath and ImageJ (NIH ...
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bioRxiv - Neuroscience 2023Quote: ... The paraffin-embedded brains and lumbar spinal cords were then serially sectioned at 10 μm thickness using a Leica RM2235 manual rotary microtome (Leica Biosystems) and mounted on poly-L-lysine microscope slides (ThermoFisher).
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Neuroscience 2023Quote: ... compound (Sakura Finetek, CA, USA) and cryo-sectioned into 10 µm thick slices using a cryostat (CM1860; Leica Biosystems, Nussloch, Germany), The sections were mounted on glass slides ...
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bioRxiv - Developmental Biology 2022Quote: ... Processed samples were then embedded in OCT (Tissue-Tek: 4583) and transverse sections 10-14μm were taken using a cryostat (Leica: CM 3050S). EdU incorporation was visualized by using a labeling mix containing Copper Sulfate Pentahydrate (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Bioengineering 2023Quote: ... was used to visualize the nano-architecture of PA hydrogel-stabilized cardiac tissue after decellularization (DECIPHER samples) and native cardiac tissue cryosections cut at 10 µm (Leica CM1950) from flash-frozen fresh young hearts embedded in OCT (Tissue-Tek ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue samples were frozen in OCT (Tissue-Tek) on dry ice and sectioned at 10 μm on a cryostat microtome (Leica CM1950). Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X-100 (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... mice were transcardially perfused with 10% formalin and coronal slices (100 μm) containing the hippocampus were cut with a vibratome (VT1000S, Leica Microsystems). For immunohistochemistry ...
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bioRxiv - Neuroscience 2024Quote: ... also known as CD31 staining and GLUT1 staining of two or three sections per segment (thoracic, lumbar and sacral) were imaged at 10× magnification using a TCS SP8 confocal laser scanning microscope (Leica, Germany). For CD31 staining ...
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bioRxiv - Bioengineering 2024Quote: ... Force maps were measured using a bio-AFM Nanowizard 4XP (Bruker-JPK, Germany) placed on a Leica DMi 8 inverted microscope with a 10× objective (Leica, Germany). A 5.73 µm melamine sphere (microParticles ...
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bioRxiv - Neuroscience 2024Quote: ... using the same acquisition settings for all brain slices and at ×10 and ×63 magnification using a Leica SP8 confocal microscope (Leica, Germany). The images were analysed by a person blinded to the genotype using Qupath and ImageJ (NIH ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using p53 polyclonal antibodies (NCL-L-p53-CM5p; Leica Biosystems). ChIPs were performed essentially as described previously (Kenzelmann Broz et al. ...
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bioRxiv - Neuroscience 2022Quote: ... slides of frontal cortex specimens stained with Clusterin antibodies were scanned on an Aperio AT2 scanner (Leica biosystems) at 20x magnification and digital slides analyzed using the QuPath platform (version 0.3.1 ...
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bioRxiv - Genetics 2020Quote: Reporter expression and fluorescent antibody-staining were imaged on an MSV269 stereoscope with a DFC365 FX camera (Leica). Freshly hatched adults (<30 minutes ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies were detected using the Bond Polymer Refine (DAB) Detection Kit (Leica Biosystems; Buffalo Grove, IL). Positive control mouse tissues for all antibodies were run in addition to negative controls performed using rat or rabbit isotype IgG.
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.
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bioRxiv - Genomics 2024Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Neuroscience 2024Quote: ... or secondary antibodies (Table 1) were acquired using the Fast Lifetime Contrast (FALCON) enabled tau-STED module (Leica) with the time-gate on HyD-X detectors adjusted between 0.1 and 6 nanoseconds.
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bioRxiv - Genetics 2021Quote: ... tissues were embedded in paraffin wax 60 (Sakura, Tokyo, Japan) and 10 μm sections were prepared using a Leica RM2125 microtome (Leica, Wetzlar, Germany). Sections were dried on glass slides overnight and then hydrated sequentially with 100% xylene ...
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bioRxiv - Neuroscience 2021Quote: ... The right hemisphere was cut into 40µm-thick sagittal sections in a series of 10 using a sliding microtome (Leica SM2010R, Wetzlar, Germany). The sections were stored in a cryoprotection solution at -20°C for further use ...
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bioRxiv - Molecular Biology 2021Quote: Frozen skin sections (thickness: 10 µm) from three Caucasian males were mounted on membrane slides (PEN-Membrane 2.0 µm, Leica Microsystems, Wetzlar, Germany) treated with 0.1 % (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Plant Biology 2021Quote: ... The percentage root colonization was obtained by analyzing 10 random stained roots under microscope at 20X (Leica Microscope. Type 020-518.500, Germany). Staining was performed by softening the sample with 10% KOH for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... Transverse sections (10 µm thickness) were taken from the middle portion of kernels within the paraffin block on a manual microtome (Leica, Heidelberg, Germany). Sections were collected on Superfrost plus charged slides (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord sections and longitudinal sciatic nerve sections were cut at 12 μm and DRGs at 10 um thickness using a cryostat (Leica Biosystems, CM3050S). Sciatic nerve and DRG sections were mounted on Superfrost+ microscope slides (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were sputter-coated with a 10-nm-thick gold-palladium (80/20) layer using a high vacuum sputter coater Leica EM SCD500 (Leica Microsystems GmbH) and visualised with a Hitachi S-4800 field emission scanning electron microscope (Hitachi High-Technologies Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings were transferred to IAA containing liquid medium (10 µM) and imaged after 24 h (Leica TCS SP8 MP).
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bioRxiv - Cell Biology 2021Quote: ... Time-lapse confocal imaging (intervals of 10 seconds per image) was perfomed on a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany) equipped with HCX PL APO λ blue 40.0×/1.25 oil objective ...
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bioRxiv - Systems Biology 2022Quote: ... were dropped under paraffin oil and their circumference measured by formula using the eye-piece graticule (10 mm = 200 parts) (Leica Microsystems, Germany). A 5μl drop was pulled around the midgut portion and MTs and a 0.3μl drop onto the CNC with the help of a capillary pull glass rod ...
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Physiology 2021Quote: ... Diaphragm bundles were processed for immunohistochemical analyses of fiber cross-sectional area and myosin heavy chain isoforms as described previously.57,61 Bundles embedded in Tissue-Tek OCT freezing medium were sliced into sections of 10 μm thickness at approximately -20ºC using a cryostat (Leica, CM 3050S model). Sections were incubated in 1:200 wheat germ agglutinin (WGA ...
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bioRxiv - Cancer Biology 2021Quote: ... The prefrozen liver tissues in OCT were transverse sectioned at a thickness of 10 µm using a CM1950 cryostat (Leica, Wetzlar, Germany). Tissue sections adhering to the Stereo-seq chip surface were incubated at 37°C for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).