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Citations for Leica :
1101 - 1150 of 1228 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then embedded in OCT media and cut into 10-μm-thick sections using a CM1900 Cryostat (Leica, Wetzlar, Germany). Primary human astrocytes were fixed with 4% PFA for 20 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using p53 polyclonal antibodies (NCL-L-p53-CM5p; Leica Biosystems). ChIPs were performed essentially as described previously (Kenzelmann Broz et al. ...
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bioRxiv - Neuroscience 2022Quote: ... slides of frontal cortex specimens stained with Clusterin antibodies were scanned on an Aperio AT2 scanner (Leica biosystems) at 20x magnification and digital slides analyzed using the QuPath platform (version 0.3.1 ...
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bioRxiv - Genetics 2020Quote: Reporter expression and fluorescent antibody-staining were imaged on an MSV269 stereoscope with a DFC365 FX camera (Leica). Freshly hatched adults (<30 minutes ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies were detected using the Bond Polymer Refine (DAB) Detection Kit (Leica Biosystems; Buffalo Grove, IL). Positive control mouse tissues for all antibodies were run in addition to negative controls performed using rat or rabbit isotype IgG.
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody binding is detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... for 30 or 40 minutes and primary antibody binding was visualized using a Polymer Refine Detection Kit (Leica). Slides were scanned with an AT2 (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and hematoxylin counterstain.
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bioRxiv - Genomics 2024Quote: ... Primary antibody binding was detected and visualized using the Leica Bond Polymer Refine Detection Kit (Leica Biosystems DS9800) with DAB chromogen and Hematoxylin counterstain ...
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bioRxiv - Genetics 2021Quote: ... tissues were embedded in paraffin wax 60 (Sakura, Tokyo, Japan) and 10 μm sections were prepared using a Leica RM2125 microtome (Leica, Wetzlar, Germany). Sections were dried on glass slides overnight and then hydrated sequentially with 100% xylene ...
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bioRxiv - Neuroscience 2021Quote: ... The right hemisphere was cut into 40µm-thick sagittal sections in a series of 10 using a sliding microtome (Leica SM2010R, Wetzlar, Germany). The sections were stored in a cryoprotection solution at -20°C for further use ...
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bioRxiv - Molecular Biology 2021Quote: Frozen skin sections (thickness: 10 µm) from three Caucasian males were mounted on membrane slides (PEN-Membrane 2.0 µm, Leica Microsystems, Wetzlar, Germany) treated with 0.1 % (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... Animals for protein loss assay were intracardially perfused with heparinized 0.01 M PBS (10 U ml-1 of heparin, Ratiopharm; ~110 mmHg pressure using a Leica Perfusion One system) for 5–10 min at room temperature until the blood was washed out ...
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bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Neuroscience 2020Quote: ... frozen with liquid nitrogen and cryo-sectioned (25 μm coronal section thickness for brains and 10 μm axial section thickness for spinal cords) using a cryostat (CM1850, Leica, Wetzlar, Germany) with a microtome blade (A35 ...
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bioRxiv - Plant Biology 2021Quote: ... The percentage root colonization was obtained by analyzing 10 random stained roots under microscope at 20X (Leica Microscope. Type 020-518.500, Germany). Staining was performed by softening the sample with 10% KOH for 15 min ...
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bioRxiv - Plant Biology 2020Quote: ... Transverse sections (10 µm thickness) were taken from the middle portion of kernels within the paraffin block on a manual microtome (Leica, Heidelberg, Germany). Sections were collected on Superfrost plus charged slides (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cord sections and longitudinal sciatic nerve sections were cut at 12 μm and DRGs at 10 um thickness using a cryostat (Leica Biosystems, CM3050S). Sciatic nerve and DRG sections were mounted on Superfrost+ microscope slides (Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were sputter-coated with a 10-nm-thick gold-palladium (80/20) layer using a high vacuum sputter coater Leica EM SCD500 (Leica Microsystems GmbH) and visualised with a Hitachi S-4800 field emission scanning electron microscope (Hitachi High-Technologies Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Plant Biology 2021Quote: ... five-day old seedlings were transferred to IAA containing liquid medium (10 µM) and imaged after 24 h (Leica TCS SP8 MP).
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bioRxiv - Cell Biology 2021Quote: ... Time-lapse confocal imaging (intervals of 10 seconds per image) was perfomed on a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany) equipped with HCX PL APO λ blue 40.0×/1.25 oil objective ...
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bioRxiv - Systems Biology 2022Quote: ... were dropped under paraffin oil and their circumference measured by formula using the eye-piece graticule (10 mm = 200 parts) (Leica Microsystems, Germany). A 5μl drop was pulled around the midgut portion and MTs and a 0.3μl drop onto the CNC with the help of a capillary pull glass rod ...
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bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
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bioRxiv - Physiology 2021Quote: ... Diaphragm bundles were processed for immunohistochemical analyses of fiber cross-sectional area and myosin heavy chain isoforms as described previously.57,61 Bundles embedded in Tissue-Tek OCT freezing medium were sliced into sections of 10 μm thickness at approximately -20ºC using a cryostat (Leica, CM 3050S model). Sections were incubated in 1:200 wheat germ agglutinin (WGA ...
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bioRxiv - Cancer Biology 2021Quote: ... The prefrozen liver tissues in OCT were transverse sectioned at a thickness of 10 µm using a CM1950 cryostat (Leica, Wetzlar, Germany). Tissue sections adhering to the Stereo-seq chip surface were incubated at 37°C for 3 min ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with calcein AM and Sir-Actin were captured at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). During the imaging ...
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Physiology 2023Quote: ... Cryosectioning of previously frozen placentas was performed with 10-mm-thick sections using a cryostat (Leica CM1860-UV, Leica Biosystems, Nussloch, Germany). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Neuroscience 2023Quote: ... The cell volume of cultured ependymocytes were visualized by calcein signals at 10-s intervals for 12 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The cell volume from all calcein positive cells in a field of recording selected of every culture dish used in experiment were included in the statistics ...
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bioRxiv - Cancer Biology 2024Quote: ... The brains were cryoprotected using a sucrose gradient (10%, 20%, and 30%) and then coronally sectioned (30 μm thick) using a cryostat (Leica Microsystems, Germany). Three sections around the midpoint of the hippocampus were selected per animal for each stain ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were cut into 50 μm slices, images captured using Carl Zeiss Axio Scan microscope (Promenade 10, Jena, Germany) or confocal scanning microscope (DMi8, Leica, Wetzlar, Germany).
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bioRxiv - Developmental Biology 2020Quote: ... P53 stabilization upon doxorubicin and Nutlin-3a treatment was confirmed by Western blotting using p53 CM5 antibody (Leica Biosystems).
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bioRxiv - Immunology 2021Quote: ... Triple immunostainings were performed by incubating the same sections with CD8 antibody (clone 4B11, 1:50 pH9, Leica Novocastra) and anti-mouse Alexa Fluor 488-coniugated secondary antibody (1:500 ...
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bioRxiv - Pathology 2019Quote: ... A 1:3000 dilution of rabbit anti-CD163 antibody for 15 min and then anti-rabbit Polymer AP (Leica) for 20 min resulting in magenta-red labeling ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Cancer Biology 2020Quote: ... live cells images were taken every 10 minutes with fixed position for 16 hours by using Leica confocal microscope (Leica TCS SP8, Leica Microsystems Ltd, Korea), and the obtained images were analyzed by Leica LAS X program.
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bioRxiv - Plant Biology 2021Quote: ... Samples were then mounted on aluminum stubs with carbon tape and sputter coated with 10 nm iridium (Leica EM ACE 600, Leica Microsystems) and imaged using a FEI Versa 3D scanning electron microscope (FEI ...
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bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
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bioRxiv - Cancer Biology 2022Quote: ... Digital images of these sections were captured with 10-100 × objectives using upright fluorescent microscopy (Leica DM6, Leica Microsystems Inc., Buffalo Grove, IL). Analyses were performed by investigators blinded to the sample identification.
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bioRxiv - Neuroscience 2020Quote: Half brains were immersion fixed in 10 % buffered formal saline (Pioneer Research Chemicals) for a minimum of 48 hours prior to being processed to wax (Leica ASP300S tissue processor). The blocks were trimmed laterally from the midline by ∼0.9-1.4 mm to give a sagittal section of the hippocampal formation ...