Labshake search
Citations for Leica :
1101 - 1150 of 1737 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Each hippocampus was placed in the groove of an agar block and 400 µm thick hippocampal slices were cut using a vibrating tissue slicer (VT 1200, Leica, Germany) and transferred to a chamber containing a carbogenated mixture of 50% d-ACSF and 50% ACSF (22.5 mM glucose ...
-
bioRxiv - Neuroscience 2021Quote: ... the brain tissues were frozen in liquid nitrogen and cut as 20 µm sections with cryostat (Leica Microsystems GmH, Wetzlar, Germany).
-
bioRxiv - Neuroscience 2020Quote: ... The cochlea was processed and embedded in OCT compound, (Tissue-Tek Sakura, Finetek, USA) and cut using a cryomicrotome (#818, Leica, Germany). Sections were frozen and subjected to antigen-retrieval using citrate buffer (#ab93678 ...
-
bioRxiv - Cancer Biology 2021Quote: ... High-resolution optical images of stained tissues were generated using an Aperio CS2 digital pathology slide scanner (Leica Biosystems, Wetzlar, Germany). Hematoxylin and Entellan® were purchased from Merck (Darmstadt ...
-
bioRxiv - Molecular Biology 2022Quote: ... The tissue was then embedded in OCT (Sakura) and cross sectioned at the middle part of gill in a cryostat (Leica CM1950). Morphology of the gill sections (10 µm thickness ...
-
bioRxiv - Molecular Biology 2022Quote: ... Brain samples were then frozen with optimum cutting temperature compound (O.C.T compound, Tissue-Tek) and cut on a cryotome (Leica CM1850, Leica Biosystems) into 30-μm thick coronal sections through the entire substantia nigra (SN ...
-
bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides, Leica Biosystems). Then the tissue was deparaffinized and digested with Protease Plus (ACD Bio ...
-
bioRxiv - Cancer Biology 2019Quote: ... the lung lobes with multiple metastases were dissected and sliced into 20 µm thick tissue sections by using a cryostat (Leica, CM3050S). The lung sections were washes 2 times with 0.01 M PBS and then incubated in Alexa 488 conjugated Lectin (4 µg/ml ...
-
bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
-
bioRxiv - Neuroscience 2019Quote: ... these inclusions were laser-cut from inverted adjacent tissue polyethylene naphthalate (PEN) membrane slides positioned on the stage of a LMD6500 (Leica Microsystems) microscope ...
-
bioRxiv - Molecular Biology 2019Quote: ... 150-µm-thick slices of the cerebellum were sectioned using a vibrating tissue slicer (Leica VT 1200S, Leica Microsystems, Wetzlar, Germany). The slices were then transferred to a suspended mesh within an incubation chamber filled with artificial cerebrospinal fluid (ACSF) ...
-
bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
-
bioRxiv - Neuroscience 2019Quote: ... The tissue section was mounted in an agar mount and sliced into several 100μm slices using a microtome (Leica, VT1000 S). The slices were incubated for 3 - 4 hr at room temperature with Cyanine-3-conjugated (Cy3 ...
-
bioRxiv - Neuroscience 2021Quote: ... Coronal sections (50-µm thick) were prepared from the tissue blocks containing the entire amygdalar region using a Leica VT1000S vibratome (Leica Microsystems). Sections were stored in a cryoprotectant antifreeze solution consisting of glycerol ...
-
bioRxiv - Neuroscience 2021Quote: ... Horizontal hippocampal slices (350 µm thick) were obtained using an oscillating tissue slicer (Microm, HM650V, Thermo Fisher Scientific, or VT1200 S, Leica). Brain slices were then transferred to a holding chamber (made in-house ...
-
bioRxiv - Physiology 2021Quote: ... Gel-embedded platelets were stained with uranyl acetate and lead aspartate followed by dehydration with ethanol and infiltrated with Epon resin in a Tissue Processor (Leica EMTP). 70nm sections were cut from blocks with a Reichert Ultracut E and imaged with a Tecnai 12 electron microscope (ThermoFisher UK).
-
bioRxiv - Neuroscience 2020Quote: ... Spinal cord sections 1.5 mm rostral and 1.5 mm caudal to the injury epicentre were placed in Tissue-Tek OCT compound (IA018; Proscitech) and then sectioned horizontally at 20 μm thickness on a cryostat (CM1850; Leica Microsystems) at −20 °C.
-
bioRxiv - Biochemistry 2022Quote: ... fresh unfixed kidneys were embedded in Tissue-Tek OCT compound (SAKURA, 83-1824, Japan) and sliced using a CM3050S cryostat (Leica, Germany). Eight-micrometer cryosections fixed with 4% paraformaldehyde for 10 min were stained with oil-red O ...
-
bioRxiv - Cancer Biology 2022Quote: ... Ki-67 immunohistochemical staining was performed by the UT Southwestern Simmons Comprehensive Cancer Center Tissue Management Shared Resource using a BOND-RX Automated Stainer (Leica Biosystems) and human-specific anti-Ki-67 antibody (Cell Signaling Technology #9027 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Direct fluorescence acquisition of labelled tissue sections was performed using an inverted confocal laser scanning microscope (Zeiss LSM 510 and Leica CF6) as described previously (9).
-
bioRxiv - Plant Biology 2023Quote: The fluorescence of the mScarlet reporter in the live transgenic tissues was observed under a fluorescent stereoscope (M205 FA, Leica, Germany) with a red fluorescence filter (Exc 540–580 nm ...
-
bioRxiv - Neuroscience 2022Quote: ... tissue blocks containing the hippocampal formation were mounted and 300 μM thick coronal sections were cut on a vibratome (Leica VT1000). Slices containing the dorsal hippocampal CA1 were placed in a chamber filled with normal aCSF containing 2 mM CaCl2 and 1 mM MgCl2 (continuously bubbled with 95% O2 and 5% CO2 ...
-
bioRxiv - Plant Biology 2022Quote: ... Ultrathin sections (90nm) were cut with a Diatome diamond knife from the embedded tissues using the Leica EM U7 Ultramicrotome (Leica Microsystems). The thin sections were then stained with 4% uranyl acetate for 20 minutes followed by lead citrate for 30 seconds ...
-
bioRxiv - Neuroscience 2022Quote: ... brains were embedded in optimum cutting temperature (OCT; Tissue-Tek) and sectioned coronally at 40 μm using a sliding microtome (Leica, HM450). For immunohistochemistry ...
-
bioRxiv - Neuroscience 2022Quote: ... Each hippocampus was placed in the groove of an agar block and 400 μm thick hippocampal slices were cut using a vibrating tissue slicer (VT 1200, Leica, Germany) and transferred to a chamber containing a carbogenated mixture of 50% d-ACSF and 50% ACSF (22.5 mM glucose ...
-
bioRxiv - Pathology 2023Quote: ... the specimens were paraffin-embedded and subsequently sectioned into 4-μm-thick tissue slices by a microtome (RM2235, Leica Microsystems Inc.). Finally ...
-
CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... tissue was frozen in liquid nitrogen and thin cryosections were cut with a Leica EM UC7 cryoultramicrotome (Leica Microsystems, Vienna, Austria). The sections were picked on Butvar – coated nickel grids ...
-
bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with half seed in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems). with vacuum infiltration ...
-
bioRxiv - Plant Biology 2023Quote: ... Paraplast blocks were then prepared with dozens of seeds in each block using the Leica Heated Paraffin Embedding Module EG1150 H with the added Leica Cold Plate for Modular Tissue Embedding System EG1150 C (Leica Biosystems) with vacuum infiltration ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were embedded in Tissue-Tek OTC compound (SAKURA) and sliced at a 50 μm thickness by the cryostat (Leica Biosystems). Free-floating slices were permeabilized and blocked with PBS containing 0.25% Triton X-100 and 5% bovine serum albumin (BSA) ...
-
bioRxiv - Neuroscience 2023Quote: ... The tissue was then dehydrated in a series of increasing concentrations of ethanol and then embedded in paraffin (Paraplast Plus, Surgipath) using the TES Valida paraffin embedding center (Medite) and Leica TP1020 automatic benchtop tissue processor (Leica Biosystems). The paraffin-embedded brains and lumbar spinal cords were then serially sectioned at 10 μm thickness using a Leica RM2235 manual rotary microtome (Leica Biosystems ...
-
bioRxiv - Bioengineering 2023Quote: ... the tissue was placed in a cryostat chamber at -20°C and sliced into 15 µm thick sections (CM1850, Leica microsystems). The FBR to the polymer coatings was assessed by staining with haematoxylin and eosin (H&E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
-
bioRxiv - Bioengineering 2022Quote: ... epithelial gap and granulation tissue areas were measured from Hematoxylin and Eosin (Cat#3801571 and Cat#3801615, respectively; Leica, Heidelberg, Germany) stained sections using morphometric image analysis (LASX ...
-
bioRxiv - Bioengineering 2023Quote: ... The images were collected from at least three sections of nerve tissues for each rat under a Leica DMi8 fluorescent microscope (Leica Microsystems). Quantitative analysis of images was performed using Image J (v1.8.0 ...
-
bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
-
bioRxiv - Developmental Biology 2022Quote: ... Processed samples were then embedded in OCT (Tissue-Tek: 4583) and transverse sections 10-14μm were taken using a cryostat (Leica: CM 3050S). EdU incorporation was visualized by using a labeling mix containing Copper Sulfate Pentahydrate (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
-
bioRxiv - Immunology 2023Quote: ... the tissues were frozen using a cryo-gel embedding compound (Surgipath Cryo-Gel Embedding Compound for Frozen Section Leica, Ref: 39475237). Tissue sections with a size of 15-20 microns were obtained using a cryostat device (Leica CM1950 Cryostat) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were waxed in liquid paraffin for 2 h and embedded to make paraffin blocks using a tissue embedder (EG1150, Leica, Germany). Four-micron sections were cut by paraffin slicer (RM2245 ...
-
bioRxiv - Neuroscience 2023Quote: ... Fluorescently labeled tissue sections were imaged on a wide field fluorescence microscope (Zeiss, Inc) or laser confocal systems (Leica SP8 system).
-
bioRxiv - Plant Biology 2023Quote: ... the root tissues were harvested and subjected to cryopreservation by rapidly freezing them using a high-pressure freezer (Leica EM PACT1) equipped with copper platelets (100 nm deep and 1.5 mm wide ...
-
bioRxiv - Plant Biology 2023Quote: ... and viable maize silk tissue were observed under a Leica TCS SP5 confocal laser scanning microscope (DM 6000B upright microscope, Leica, Germany) at the Molecular and Cellular Imaging Facility at the University of Guelph ...
-
bioRxiv - Molecular Biology 2023Quote: ... Each z-stack acquired tissue volume (2 µm spacing) with 200 Hz line frequency using LAS X (Leica Application Suite X).
-
bioRxiv - Neuroscience 2023Quote: ... The brain tissue containing CN was sliced into 200-300 µm thick sections parasagittally in the NMDG solution with a microtome (Leica, VT1200). The slices were transferred into the oxygenated NMDG solution (34 ± 0.5 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... they were embedded in OCT compound (Tissue-Tek, Sakura) and transverse sections of 30 μm thickness were cut in a cryostat (Leica cm1950) and collected on gelatinized slides ...
-
bioRxiv - Systems Biology 2023Quote: Frozen serial 8 μm sections of mice heart tissues were cut at −20°C with a Microtome (CM1950; Leica, Nussloch, Germany) and mounted on ITO-coated glass slides for MALDI-MSI and on coated glass slides for HE staining ...
-
bioRxiv - Genomics 2023Quote: LED-based widefield imaging of tissues was performed using the THUNDER Imager 3D Live Cell and 3D Cell Culture (Leica Microsystems). Low magnification images were obtained in combination with a 20x objective (NA ...
-
bioRxiv - Neuroscience 2023Quote: ... The tissue samples were frozen in OCT (Tissue-Tek) on dry ice and sectioned at 10 μm on a cryostat microtome (Leica CM1950). Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma ...
-
bioRxiv - Genetics 2024Quote: ... Tissue sections were sealed with a DAPI-containing sealer (Beyotime, Shanghai, China) to visualize the gonadal tissue nuclei and photographed under a fluorescent inverted microscope (Leica, Germany).