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Citations for Leica :
1051 - 1100 of 1737 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... snap-frozen livers were embedded in Tissue-Tek® O.C.T (Sakura: 4583) and cut in 10 μm sections using Leica Cryostat (Leica Biosystems). 5 sections in a distance of 100 μm to each other were stained per liver ...
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bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
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bioRxiv - Immunology 2023Quote: Morphometric analysis was performed on H&E-stained paraffin tissue sections using light microscopy equipped with a KY-F75U digital camera (JVC) at various magnifications (Leica DM4000B ...
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bioRxiv - Neuroscience 2023Quote: ... the brain tissue was attached to a circular cryostat block and sectioned on a cryostat (Leica CM1860, Leica, Wetzlar, Germany) into 40-μm-thick slices at -17 to -20°C ...
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bioRxiv - Bioengineering 2023Quote: ... Immunostained cells and tissues were imaged on the Evos Revolve widefield microscope using a 20x objective or Stellaris 5 confocal microscope (Leica) with a 10x or 25x water-immersion objective to capture data in DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... the brain tissue was attached to a circular cryostat block and sectioned on a cryostat (Leica CM1860, Leica, Wetzlar, Germany) into 40-μm-thick slices at -17 to -20°C ...
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bioRxiv - Microbiology 2023Quote: ... The tissues were then placed on a slide and visualized immediately using a Leica SP5 confocal microscope (Leica Microsystems, USA). The images were analyzed with the ImageJ 1.50i software package (30).
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections of 30 µM thickness and 600 µM apart were sectioned using a Leica cryostat (Leica Biosystems, Wetzlar, Germany), starting at -0.5 anterior-posterior (AP ...
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bioRxiv - Physiology 2023Quote: ... mouse anterior quadrants were embedded in Epon resin and 65 nm sagittal ultrathin sections were cut through iridocorneal angle tissues using an ultramicrotome (LEICA EM UC6, Leica Mikrosysteme GmbH ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf tissues were transferred to 70% glycerol and viewed using the AS Laser Microdissection system microscope (Leica Microsystems, Deerfield, IL). Note that trypan blue also slightly stains fungal structures.
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bioRxiv - Physiology 2023Quote: ... mouse anterior quadrants were embedded in Epon resin and 65 nm sagittal ultrathin sections were cut through iridocorneal angle tissues using an ultramicrotome (LEICA EM UC6 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were cast into molds together with liquid paraffin and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate) ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was washed in PB and sectioned coronally in a vibratome (Vibratome Sectioning System, VT1200S Vibratome, Leica Biosystems, Germany). Sections containing all layers of the EC were selected and processed for Nissl staining to determine the cytoarchitecture (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... The brains were embedded in OCT tissue TEC (Sakura) and 30 µm-thick frozen coronal sections were cut using a model CM 3050 cryostat (Leica). The sections were blocked with blocking buffer for 1 h and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Bioengineering 2024Quote: ... and fixed tissues/embryos (using a 10x objective) employing the Leica TCS SP8 confocal laser scanning microscope (CLSM) from Leica Microsystems ...
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bioRxiv - Genetics 2023Quote: ... the eyes underwent rehydration using a series of ethanol gradients with the assistance of an automated tissue processor (ASP300S, Leica). Afterward ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed tissues were embedded in CryoMount I (Muto PureChemicals) and 8 μm-thick sections were cut using a cryostat (Leica). For immunofluorescence assay ...
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bioRxiv - Neuroscience 2024Quote: ... The fixed tissue block was sectioned with a 50 μm step-size on a vibratome (VT 1000P, Leica, Wetzlar, Germany), and sections were stored in a cryoprotectant solution (30% ethylene glycol and 30% glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... Acute sagittal slices (250 um) of the cerebellum containing intact Purkinje cells were obtained using a vibrating tissue slicer (VT1200S; Leica) and incubated in ACSF (125 mM NaCl ...
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bioRxiv - Physiology 2024Quote: Cardiac tissue was embedded in Optimal Cutting Temperature (OCT) (Tissue Tek) and cut into 8 μm thick using a cryostat (CM1850; Leica) with the temperature set as −20°C ...
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bioRxiv - Neuroscience 2023Quote: Brains of WT and RIM-BP2 KO mice were quickly removed and transferred to cryomold (Sakura Finetek) filled with tissue freezing medium (Leica). Brains were instantaneously frozen on aluminum block in liquid nitrogen and stored at -80°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and then incubated in succession in 20% and 30% sucrose solution for at least 24 hours each before being embedded in Tissue freezing medium (Leica). Histological and immuno-fluorescence/histochemistry experiments were carried out on 6 μm sections ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue visualization and image collection was performed using a Leica Stellaris 5 Confocal microscope and LAS X software (Leica Microsystems). All images were acquired using 40X (HC PL APO CS2 ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human CD163 mAb (clone, 10D6; Leica Biosystems, Buffalo Grove, IL), rabbit anti-mouse β-catenin pAbs (Proteintech ...
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bioRxiv - Cancer Biology 2021Quote: For immunohistochemical validation studies anti-CD3 (mouse monoclonal, clone LN10, Leica Microsystems) and anti-CD15 (mouse monoclonal ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse was then secured on a stereotaxic frame (Angle Two, Leica Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: Six GFAP immunoreactivity light microscope images per mouse were acquired by Leica DM6B-Z Thunder Imager microscope (Leica Microsystems CMS GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... 300 μm-thick coronal slices containing caudate putamen (CPu) and NAc were prepared from dissected brain tissue using a vibratome (VT1200S, Leica Microsystems) and transferred to a holding chamber containing a HEPES-based buffer solution maintained at room temperature (20-22°C ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were embedded in the same Tissue Freezing Medium and sectioned with 16 μm thickness on a cryostat (Leica CM 3050S). Slides were incubated overnight at 4 °C and washed with PTW for rehydration ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were washed in PTW and incubated once overnight in 30 % sucrose in PTW and once in Tissue Freezing Medium (Leica, #14020108926): 30 % Sucrose in PTW (1:1 ...
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bioRxiv - Neuroscience 2022Quote: ... osmolarity 280-320 mOsm/l) was used both to keep tissue cold during dissection and as the bathing solution during vibratome slicing (Leica VT1200S). 0.4-1 mm-thick dorsal OB slices were taken by near-horizontal slicing ...
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bioRxiv - Molecular Biology 2020Quote: ... Brains were embedded in optimum cutting temperature (Tissue-Tek) and coronally sectioned at 40 μm using a sliding microtome (Leica, HM450). For immunohistochemistry ...
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bioRxiv - Genomics 2020Quote: ... Areas of interest were annotated on tissue scans and isolated with a microscope equipped with a laser-capture microdissection system (Leica LMD7). Microbiopsies were collected in 96-well plates and lysed using the Arcturus PicoPure Kit (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... H2O2 was detected as a reddish-brown colouration in the DAB treated tissue and visualised under a light microscope (Leica DM750).
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bioRxiv - Physiology 2020Quote: ... tissues were mounted in Vectashield with DAPI and examined under a fluorescent confocal microscope (Leica, TCS SP5, Leica Biosystems, Bretton, Peterborough).
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bioRxiv - Neuroscience 2019Quote: ... the initial incubation steps (until dehydration) were performed with the aid of an automatic microwave tissue processor (Leica EM AMW, Leica, Germany) for V2 ...
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bioRxiv - Bioengineering 2021Quote: ... the paraffin-embedded block tissues were sectioned at the surface with ∼7-µm thickness by a microtome (RM2235, Leica Microsystems Inc.). The thin tissue slices were stained by H&E ...
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Epicardial slices: a 3D organotypic model for the study of epicardial activation and differentiationbioRxiv - Bioengineering 2020Quote: ... embedded tissue cubes were mounted with the epicardium face up onto the specimen holder of a high precision vibratome (Leica, VT1200S) and cut to produce slices of 400-500µm thickness ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed in PB and coronally sectioned (150μm thick) in a vibratome (Vibratome Sectioning System, VT1200S Vibratome, Leica Biosystems, Germany). Sections containing EC were selected for Nissl staining ...
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bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
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bioRxiv - Plant Biology 2021Quote: ... The fixed grains were dehydrated by a graded ethanol series and xylene before infiltrating them with paraffin wax (Paraplast Plus) in an automated tissue processor (TP1020, Leica Biosystems). The grains were embedded in paraffin wax on an embedding centre (EG1150 ...
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bioRxiv - Neuroscience 2020Quote: ... The tissues were then sectioned through occipito-temporal region containing hippocampus (14 µm thickness) using a cryotome machine (Leica CM1900, Germany) followed by storage at -20°C for immunohistochemical analysis.
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bioRxiv - Cancer Biology 2020Quote: ... and after two washing steps with PBS the nonspecific binding of immunoglobulins was prevented by incubating the tissue slices with the Protein Block Novocastra Solution (Leica Biosystems) for 60 min ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissue was kept on ice-cold aCSF and tissue sections containing LHb were sectioned at 220μm using a vibratome (Leica; Wetzler, Germany) and subsequently incubated in aCSF at 34 °C for at least 1-hr prior to electrophysiological experiments ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fixed samples were embedded in Technovit 8100 (Kulzer, Germany) and processed into 2 μm tissue sections using a rotary microtome RM2255 (Leica, Germany). The AlexaFluor555-labeled oligonucleotide probes Eco934 (5’-CAT GCT CCA CCG CTT GTG-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then frozen in −20°C isopentane and cut into 20 μm-thick coronal sections on a cryostat (Leica CM1900), collected on silane-coated slides and stored at −20°C ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was washed in PB and sectioned coronally in a vibratome (150μm thickness; Vibratome Sectioning System, VT1200S Vibratome, Leica Biosystems, Germany). Sections containing EC were selected and processed for Nissl-staining and immunocytochemistry to determine cytoarchitecture (Fig ...