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Citations for Leica :
1001 - 1050 of 1209 citations for IL 13 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... GFPdgn direct fluorescence (green) and the stained F-actin (red) were visualized and imaged using a Leica TCS SP8 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cancer Biology 2019Quote: ... These cross-sections of the lungs were stained with H&E and scanned by an Aperio ScanScope® CS System (Leica Biosystems, Buffalo Grove, IL). The metastatic burden was calculated as the percentage of metastasis/lung area and the number of foci/lung area ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Physiology 2021Quote: ... All sections of both patellae and femora were stained with Safranin-O/Fast Green and imaged at 20x magnification using a slide imaging system (Aperio, Leica 122 Biosystems, Buffalo Grove, IL). Two blinded raters scored each section using the Osteoarthritis Research Society International (OARSI ...
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The z-stack images of the whole tissue were acquired with a 5 mm step size in a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Images were segmented and volumetrically reconstructed with Imaris software (Bitplane ...
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bioRxiv - Biochemistry 2022Quote: ... The grid is immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunger (Leica, EM GP, Buffalo Grove, IL, USA). The vitrified specimens are analyzed using a FEI Tecnai 12 G2 TEM at 120 kV with a Gatan cryo-holder maintained at −180 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: Interdigitation analysis was performed on cryosections stained with Hematoxylin and Eosin as previously described [74] and imaged using an Aperio ScanScope (Leica Biosystems, Buffalo Grove, IL, USA) and analyzed using Aperio ImageScope v12 software (Leica Biosystems ...
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bioRxiv - Plant Biology 2021Quote: Leaves were cut into small pieces and placed on glass slides and observed with a Leica TCS SP5 confocal laser scanning microscope system (Leica Micro-systems, Bannockburn, IL, USA). GFP fluorescence was detected with excitation at 488nm and emission capture at 500–530 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The double staining was done sequentially on a Leica Bond-IIITM instrument using the Bond Polymer Refine DAB Detection System (Leica Microsystems DS9800, Buffalo Grove, IL) and Refine Red Detection System (Leica Microsystems DS9390) ...
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bioRxiv - Developmental Biology 2022Quote: ... pilot experiments confirmed single cell dissociation with this protocol by visual inspection using the DIC feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). All groups of dissociated cells had over 80% viability and were diluted to a final concentration of 3,500 cells/ul in 0.04% BSA/HBSS for cDNA library preparation ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Spontaneous contractions of cardiomyocytes were recorded real-time under phase contrast microscopy using a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Molecular Biology 2021Quote: ... High-resolution Stimulated Emission Depletion (STED) images were acquired on Leica TCS SP5 II STED-CW laser scanning confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Emission depletion was accomplished with 592 nm STED laser ...
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were frozen on crushed dry ice and then stored at −80 °C until sliced into 20 μm coronal sections in a cryostat (Leica Biosystems 1850, Buffalo Grove, IL) at −16 to −18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut down the midline and each half vibratome sectioned into 40 µm sagittal slices using a Leica Vibratome (VT1000S; Leica Biosystems Inc., Buffalo Grove, IL) and stored in PBS at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were cut at 10 μm using a cryostat and sections containing the striatum and SNc/ventral tegmental area (VTA) were collected on Leica PET FrameSlides (Leica Microsystems, Buffalo Grove, IL, USA). Slides were stored at −80 °C until staining.
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bioRxiv - Neuroscience 2020Quote: ... which shows expression in uninjured axons.25 All immunostains were performed using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of treated embryos were stained with Hoechst 33342 Dye and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of quail at HH27 were stained with Hoechst 33342 Dye (see below) and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2021Quote: ... GAD65 perisomatic puncta as well as levels of PV present in hM4DGi-mCherry and mCherry-expressing cells in the prelimbic region of the PFC were estimated from analysis of 10x images acquired on a Leica confocal microscope (Leica Microsystems, Buffalo Grove, IL, USA) of sections stained for either PV and mCherry or GAD65 and MAP2 ...
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bioRxiv - Neuroscience 2020Quote: ... All stained slides were scanned at 20x magnification into digital images using the Aperio scanner system (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems Inc., Richmond, IL, USA) for analysis.
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... Sections of tumors were H&E and von Kossa stained and scanned using an Aperio AT2 digital whole-slide scanning system (Leica Biosystems, Buffalo Grove, IL, USA). Fluorescence was observed under the fluorescence microscope as described above.
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bioRxiv - Microbiology 2022Quote: ... Expression of pUL47eGFP was examined using a Leica M205 FCA fluorescent stereomicroscope with a Leica DFC7000T digital color microscope camera (Leica Microsystems, Inc., Buffalo Grove, IL) as previously described [23 ...
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bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
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bioRxiv - Cancer Biology 2024Quote: ... were selected based on careful annotation of H&E-stained slides that were digitally acquired using the Aperio ScanScope Turbo slide scanner (Leica Microsystems Inc., Buffalo Grove, IL). HALO software (Indica Labs ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed for 24 h and 80μm coronal sections containing the NAcore were collected using a vibrating microtome (Leica Biosystems Inc, Deer Park, IL, USA). For structural plasticity analyses (Experiment 2) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... which were then quantified for positivity (Npositive/Ntotal) to account for potential differences in collagen deposition in Aperio Imagescope (Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... We sectioned brains on a coronal plane at 150 μm using a cryostat at -20°C (Leica CM3050 S, Leica Biosystems Inc., Buffalo Grove, IL) and stored the sections at -70°C until we dissected the medial prefrontal cortex ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Bioengineering 2022Quote: The Raman spectra of the organoids were acquired by using a Renishaw inVia Confocal Raman spectrometer connected to a Leica microscope (Leica DMLM, Leica Microsystems, Buffalo Grove, IL, USA). A 785 nm near-IR laser ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2023Quote: Layer 5 of somatosensory cortex was imaged at 33 frames per second for 10 minutes on a Leica DMI 6000B microscope (Leica Microsystems, Inc., Buffalo Grove, IL) with an Andor CSU W1 confocal spinning disc unit ...
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bioRxiv - Immunology 2023Quote: ... The fixed samples were washed with 70% ethanol and embedded in paraffin by TP1020 Leica semi-enclosed benchtop tissue processor (Leica biosystems, Buffalo Grove, IL, USA) and sectioned with microtome (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were flash frozen in a slurry of dry ice and isopentane and sectioned on a cryostat (Leica CM 1860 Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... At day EP14 slices were cut out along with the membrane and placed into the perfusion chamber mounted on a spinning disc microscope (Leica DMI 6000B (Leica Microsystems, Inc., Buffalo Grove, IL), with an Andor CSU W1 spinning disc unit ...
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bioRxiv - Neuroscience 2022Quote: ... USA) to a stainless-steel slicing chuck and 350 μm transverse slices were made using the Leica 1000 vibratome (Leica Microsystems, Buffalo Grove, IL, USA) as described previously (Quinlan et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... for electron microscopy analysis in coronal sections on a sliding microtome equipped with a freezing stage (Leica SM 2010R, Leica Biosystems, Deer Park, IL, USA). Slices for immunofluorescence staining were stored at −20°C in antifreeze solution (20% Glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... injury on the left hemisphere at the primary motor and somatosensory cortex using an electromagnetic Impact One stereotaxic impactor (Leica Microsystems, Buffalo Grove, IL, USA). The impact site was localized at 2 mm lateral and 2 mm posterior to Bregma with a 3 mm diameter flat impact tip ...
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bioRxiv - Biochemistry 2024Quote: ... and finally incubated with 3 μM MitoSOX Red for 10 min and fixed with paraformaldehyde (2%) and analyzed by fluorescence microscopy (Leica TCS SP5, Buffalo Grove, IL).
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bioRxiv - Neuroscience 2020Quote: Fixed mouse brains were sectioned on a sliding microtome (Leica) at 40μm ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-CD163 (clone 10D6, Leica Biosystems, catalog number PA0090) ready-to-use solution (30 minutes) ...
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bioRxiv - Neuroscience 2020Quote: ... or 1:250 mouse anti-Ki67 (Leica Biosystems; Newcastle, UK), 1% NDS ...