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Citations for Leica :
951 - 1000 of 1146 citations for IL 13 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... INS-1E cells were incubated with HO-PI for 15 min (5 mg/ml) and observed using a confocal laser-scanning microscope (Leica SP5; Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The z-stack images of the whole tissue were acquired with a 5 mm step size in a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Images were segmented and volumetrically reconstructed with Imaris software (Bitplane ...
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bioRxiv - Biochemistry 2022Quote: ... The grid is immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunger (Leica, EM GP, Buffalo Grove, IL, USA). The vitrified specimens are analyzed using a FEI Tecnai 12 G2 TEM at 120 kV with a Gatan cryo-holder maintained at −180 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Cell Biology 2021Quote: Interdigitation analysis was performed on cryosections stained with Hematoxylin and Eosin as previously described [74] and imaged using an Aperio ScanScope (Leica Biosystems, Buffalo Grove, IL, USA) and analyzed using Aperio ImageScope v12 software (Leica Biosystems ...
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bioRxiv - Plant Biology 2021Quote: Leaves were cut into small pieces and placed on glass slides and observed with a Leica TCS SP5 confocal laser scanning microscope system (Leica Micro-systems, Bannockburn, IL, USA). GFP fluorescence was detected with excitation at 488nm and emission capture at 500–530 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The double staining was done sequentially on a Leica Bond-IIITM instrument using the Bond Polymer Refine DAB Detection System (Leica Microsystems DS9800, Buffalo Grove, IL) and Refine Red Detection System (Leica Microsystems DS9390) ...
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bioRxiv - Developmental Biology 2022Quote: ... pilot experiments confirmed single cell dissociation with this protocol by visual inspection using the DIC feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL). All groups of dissociated cells had over 80% viability and were diluted to a final concentration of 3,500 cells/ul in 0.04% BSA/HBSS for cDNA library preparation ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Spontaneous contractions of cardiomyocytes were recorded real-time under phase contrast microscopy using a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Molecular Biology 2021Quote: ... High-resolution Stimulated Emission Depletion (STED) images were acquired on Leica TCS SP5 II STED-CW laser scanning confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Emission depletion was accomplished with 592 nm STED laser ...
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were frozen on crushed dry ice and then stored at −80 °C until sliced into 20 μm coronal sections in a cryostat (Leica Biosystems 1850, Buffalo Grove, IL) at −16 to −18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut down the midline and each half vibratome sectioned into 40 µm sagittal slices using a Leica Vibratome (VT1000S; Leica Biosystems Inc., Buffalo Grove, IL) and stored in PBS at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were cut at 10 μm using a cryostat and sections containing the striatum and SNc/ventral tegmental area (VTA) were collected on Leica PET FrameSlides (Leica Microsystems, Buffalo Grove, IL, USA). Slides were stored at −80 °C until staining.
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bioRxiv - Neuroscience 2020Quote: ... which shows expression in uninjured axons.25 All immunostains were performed using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of treated embryos were stained with Hoechst 33342 Dye and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Developmental Biology 2021Quote: ... The heads of quail at HH27 were stained with Hoechst 33342 Dye (see below) and imaged on a stereo dissection microscope under epifluorescent illumination (MZFLIII-TS, Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2021Quote: ... GAD65 perisomatic puncta as well as levels of PV present in hM4DGi-mCherry and mCherry-expressing cells in the prelimbic region of the PFC were estimated from analysis of 10x images acquired on a Leica confocal microscope (Leica Microsystems, Buffalo Grove, IL, USA) of sections stained for either PV and mCherry or GAD65 and MAP2 ...
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bioRxiv - Neuroscience 2020Quote: ... All stained slides were scanned at 20x magnification into digital images using the Aperio scanner system (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems Inc., Richmond, IL, USA) for analysis.
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... Sections of tumors were H&E and von Kossa stained and scanned using an Aperio AT2 digital whole-slide scanning system (Leica Biosystems, Buffalo Grove, IL, USA). Fluorescence was observed under the fluorescence microscope as described above.
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bioRxiv - Microbiology 2022Quote: ... Expression of pUL47eGFP was examined using a Leica M205 FCA fluorescent stereomicroscope with a Leica DFC7000T digital color microscope camera (Leica Microsystems, Inc., Buffalo Grove, IL) as previously described [23 ...
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bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Bioengineering 2022Quote: The Raman spectra of the organoids were acquired by using a Renishaw inVia Confocal Raman spectrometer connected to a Leica microscope (Leica DMLM, Leica Microsystems, Buffalo Grove, IL, USA). A 785 nm near-IR laser ...
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bioRxiv - Neuroscience 2023Quote: Layer 5 of somatosensory cortex was imaged at 33 frames per second for 10 minutes on a Leica DMI 6000B microscope (Leica Microsystems, Inc., Buffalo Grove, IL) with an Andor CSU W1 confocal spinning disc unit ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Immunology 2023Quote: ... The fixed samples were washed with 70% ethanol and embedded in paraffin by TP1020 Leica semi-enclosed benchtop tissue processor (Leica biosystems, Buffalo Grove, IL, USA) and sectioned with microtome (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were flash frozen in a slurry of dry ice and isopentane and sectioned on a cryostat (Leica CM 1860 Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... At day EP14 slices were cut out along with the membrane and placed into the perfusion chamber mounted on a spinning disc microscope (Leica DMI 6000B (Leica Microsystems, Inc., Buffalo Grove, IL), with an Andor CSU W1 spinning disc unit ...
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bioRxiv - Neuroscience 2022Quote: ... USA) to a stainless-steel slicing chuck and 350 μm transverse slices were made using the Leica 1000 vibratome (Leica Microsystems, Buffalo Grove, IL, USA) as described previously (Quinlan et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Cancer Biology 2023Quote: ... which were then quantified for positivity (Npositive/Ntotal) to account for potential differences in collagen deposition in Aperio Imagescope (Leica Biosystems, Buffalo Grove, IL, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... We sectioned brains on a coronal plane at 150 μm using a cryostat at -20°C (Leica CM3050 S, Leica Biosystems Inc., Buffalo Grove, IL) and stored the sections at -70°C until we dissected the medial prefrontal cortex ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed for 24 h and 80μm coronal sections containing the NAcore were collected using a vibrating microtome (Leica Biosystems Inc, Deer Park, IL, USA). For structural plasticity analyses (Experiment 2) ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... were selected based on careful annotation of H&E-stained slides that were digitally acquired using the Aperio ScanScope Turbo slide scanner (Leica Microsystems Inc., Buffalo Grove, IL). HALO software (Indica Labs ...
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bioRxiv - Neuroscience 2020Quote: Fixed mouse brains were sectioned on a sliding microtome (Leica) at 40μm ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-CD163 (clone 10D6, Leica Biosystems, catalog number PA0090) ready-to-use solution (30 minutes) ...
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bioRxiv - Neuroscience 2020Quote: ... or 1:250 mouse anti-Ki67 (Leica Biosystems; Newcastle, UK), 1% NDS ...
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bioRxiv - Microbiology 2022Quote: Staining for LANA (Leica mouse monoclonal PA0050 ready-to-use) and CD31 (Abcam rabbit polyclonal ab32457;1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
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bioRxiv - Molecular Biology 2024Quote: ... #5032) and mouse p53 (NCL-L-p53-CM5p, Leica Biosystems).
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bioRxiv - Neuroscience 2023Quote: PVH sections (10-12) from OXT-hM4Dgi-mcherry injected rats were imaged on a confocal microscope (Leica SP5 DMI, Leica Micro-Systems, Buffalo Grove, IL, USA) at the Microscopy and Advanced Bioimaging CoRE at the Icahn School of Medicine at Mount Sinai ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-Ki67 (1:400, mouse, “MM1”, Leica, NCL-L-Ki67-MM1), anti-MCM2 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was incubated with primary antibody against mouse p53 (CM5, Leica) overnight followed by two hours of conjugation to Protein A+G conjugated magnetic beads ...
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bioRxiv - Cell Biology 2020Quote: Mouse anti–Emerin (1:100; clone 4G5; EMERIN-CE; Leica Biosystems)