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Citations for Leica :
951 - 1000 of 1064 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2023Quote: ... After the brain samples were fixed in 4% paraformaldehyde overnight, they were dehydrated in 30% sucrose (Daejung, Korea) and embedded in frozen section compound (Leica, Germany). Coronal sections (10 µm ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000; Ted Pella, or VT1000P; Leica). Sections were collected in cryoprotectant (25% glycerol ...
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bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Immunology 2023Quote: ... from Apoe-/-LysMCre and Apoe-/- LysMCreCnr1flox/flox male mice collected after 4 weeks WD were mounted on RNase-free membrane metal frame slides (POL FrameSlide, Leica Microsystems). Following deparaffinization under RNase-free conditions ...
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bioRxiv - Neuroscience 2024Quote: ... and LHA (for recordings) were cut in ice-cold (4 °C) choline chloride ACSF using a Leica VT1000 vibratome (Leica Biosystems) and transferred to warm (33 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse brains were post-fixed in 4% PFA overnight and sectioned coronally at 50 µm thickness (approximately 240 sections per brain) on a vibratome (Leica VT1200S). Brain sections containing regions of interest were permeabilized in 1% Triton X-100 in TBS and blocked in 5% normal goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... brains were post-fixed in 4% PFA at 4 °C and then rinsed in PBS before being sectioned at a thickness of 60 µm using a vibratome (Leica Biosystems). These sections were then mounted onto Superfrost Plus slides (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were removed and postfixed overnight at 4°C and then cut into 50 µm coronal sections using a vibratome (#TPI1000, Ted Pella, or VT1000P, Leica). Sections were collected into cryoprotectant (25% glycerol ...
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bioRxiv - Developmental Biology 2024Quote: ... at room temperature, incubated with 20% sucrose (Fisher BioReagents, BP220-1) overnight at 4°C and then embedded in OCT (Leica, 14020108926) and stored at −80°C until further use.
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C with the following dilutions in blocking buffer: p53 (CM5) (1:300, NCL-L-p53-CM5p, Leica Biosystems), Cleaved Caspase-3 (Asp175 ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were imaged in brightfield using a Point Grey Flea®3 color camera (Teledyne FLIR, Wilsonville, OR) mounted on a Leica S8 APO stereo microscope (Leica Camera, Wetzlar, Germany). Representative images are shown.
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bioRxiv - Pathology 2021Quote: Four micron thick sections of left and right ventricular FFPE tissue blocks per decedent were cut and labeled using an automated staining platform (Leica Bond 3, Leica Biosystems, Buffalo Grove, IL) with the following monoclonal antibodies against ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were rinsed 3 times in phosphate buffered saline and 50 μM coronal brain slices were obtained using a vibratome (Leica VT1000, Buffalo Grove, Il), without embedding the tissue ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed an additional 3 times in 100% ethanol for 15 minutes each and dried using a Leica EM CPD030 critical point dryer (Leica Microsystems, Buffalo Grove, IL) with CO2 as the transitional medium ...
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bioRxiv - Bioengineering 2023Quote: ... were acquired over a ∼30 mm x ∼3 mm rectangular area by using the tile scan feature of the LAS X software (Leica Microsystems, Buffalo Grove, IL). This involved frame by frame acquisition (36 × 5 = 180 frames ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Cancer Biology 2021Quote: ... and red tdTomato/CMDiI channels and were recorded as approximately 1 x 4 stitches at 10x magnification using a Leica sp8 confocal microscope (Leica, Wetzlar, Germany). Consecutive stitch sequences were processed into a single image using Fiji 33 using the plugin by Preibisch et al. ...
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bioRxiv - Bioengineering 2020Quote: ... eyes were fixed in 4% paraformaldehyde (w/v) in PBS for 1 hour and embedded in optimal cutting temperature compound (Leica Biosystems, Germany) and stored at -80C until cryosectioning ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...