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Citations for Leica :
801 - 850 of 1064 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: Pancreas were dissected and directly fixed in 4% paraformaldehyde overnight before embedding in paraplast (Paraplast X-tra, 39603002, Leica). 6 μm-thick sections were produced ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffinized tissue blocks were sliced at a 4-μm thickness using a Sliding Microtome SM2000R (Leica Biosystems, Wetzlar, Germany) and processed for IHC staining ...
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bioRxiv - Bioengineering 2024Quote: ... embedded in 4 % agarose and cut at a thickness of 150 µm using a vibratome (VT1000S vibratome, Leica Biosystems). Brain slices were stained with Hoechst 33342 (10 µg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... flattened hemispheres were embedded in agar gel 4% before cutting slices of 60µm thickness with a vibratome (VT 1000 S, Leica).
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% agarose/10% sucrose and sectioned at 50 µm using a vibratome (Leica VT1200S). Sections were stored at −20°C in a glycol mixture until use ...
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bioRxiv - Neuroscience 2024Quote: ... The samples embedded in paraffin were sagittally sectioned at 4 μm using a microtome HistoCore Biocut (Leica, Madrid, Spain). Sections corresponding to similar brain coordinates were deparaffined and rehydrated ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting resin blocks were then sectioned into 4 μm slices using a Leica Histocore AUTOCUT rotary microtome (Leica) and stained with either 0.01% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... The embedded tissues were sectioned to 3 μm-thick sections and stained with Haematoxylin-Erythrosin-Saffron (HES) before visualizing under a Leica DMRBE light microscope (Leica Microsystems, Germany). The stained sections were scanned with a Hamamatzu NanoZomer S60 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm segments from the root tip were embedded in 3% agarose and were sectioned at 150-200 μM using a vibratome (Leica VT1000 S). The sections were stained in Clearsee buffer (Ursache et al ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... At least 3 pictures/section were acquired at 40x and 63x magnification by DMI600 wide field fluorescence microscope (Leica microsystems, Wetzlar, Germany). Hoechst signal (cell nucleus ...
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bioRxiv - Neuroscience 2021Quote: ... The STG or stn near its entrance to the STG was then illuminated for 3-7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica, Wetzlar, Germany). Complete photoinactivation of LP was confirmed when the membrane potential reached 0 mV ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Neuroscience 2024Quote: Paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 30 NCs) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany) (46 ...
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bioRxiv - Developmental Biology 2024Quote: ... Brains were embedded in 3% low-melting point (LMP) agarose and sectioned coronally at 100 μm using a vibratome (Leica, model VT1200). Free-floating sections were incubated in block solution (2% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Plant Biology 2022Quote: ... The roots were washed in water and one cm segments from the root tip were embedded in 3% agarose and made sections of 150-200 μM using a vibratome (Leica VT1000 S). Confocal Laser Scanning microscopy was performed on a Zeiss LSM700 confocal with the 20X objective ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were rinsed in 0.1 M phosphate buffer (PB; 3 × 10 min) and 50-70 μm-thick coronal sections were obtained with a vibrating blade microtome (Leica VT1000 S).
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Cancer Biology 2024Quote: All stained sections except for those from experiments involving Lepob mice (Fig. 3) were scanned at 20x magnification using a Leica Aperio AT2 slide scanner (Leica Microsystems, UK) prior to subsequent analysis of scanned slides as described below.
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bioRxiv - Biophysics 2024Quote: ... grids were applied with 3 μL 10 nm diameter BSA Gold Tracer (Aurion, The Netherlands) and single-side blotted using the EMGP plunger (Leica, Wezlar, Germany). Subsequent imaging of vitrified grids was performed on a Titan Krios microscope (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae subjected to TBI (as described above) at 3 dpf were anesthetized at 7 dpf with MS-222 and imaged under a fluorescent dissecting scope (Leica M165 FC). GFP+ puncta were counted from a lateral view (blinded).
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bioRxiv - Neuroscience 2024Quote: ... coli) and transferred onto 3% (w/v) agarose pads on a glass slide for confocal microscopy study (TCS SPE, Leica, Wetzlar, Germany). The excitation wavelength was set at 488 nm (Jex ∼488 nm) ...
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bioRxiv - Neuroscience 2024Quote: The paraffin-embedded fixed postmortem human brain tissues (from 34 AD patients and 31 NC subjects) were cut into 3 µm sections using a Leica microtome (Leica Microsystems, Germany). Hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2024Quote: ... The immunostaining on each slice (3 sections per slice) was imaged using the Leica TCS SPE-II laser scanning confocal microscope (Leica, Wetzlar, Germany) and averaged per rat.
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of each sample were plunged into liquid ethane with a Leica EM GP automatic plunge freezer (Leica Microsystems, Wetzlar, Germany). The plunging chamber was set to have the following conditions ...
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bioRxiv - Cell Biology 2024Quote: ... adjusted pH 7.25–7.3 with 10 N NaOH to set Na 154 mM) using Leica VT1200 S fully automated vibrating-blade microtome (Leica, Wetzlar, Germany). Sections were kept at 37 °C for 20 min and then allowed to return to room temperature for the duration of the experiment ...
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bioRxiv - Biophysics 2021Quote: ... and incubated for 10 s prior to blotting on Whatman 1 paper for 4 s and plunge-frozen in liquid ethane using a GP2 plunge freezer (Leica) equilibrated to 10°C and 85% humidity ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by immersion in 30% sucrose at 4°C sucrose and then embedded in an OCT block for cryosectioning at 35μm thickness (Leica, Germany) and used for histological studies.
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bioRxiv - Evolutionary Biology 2021Quote: ... 300µm-thick acute coronal brain sections were cut after embedding in 4% low melt-agar using a vibratome (Leica, #VT1000S) under RNase-free conditions ...
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bioRxiv - Neuroscience 2020Quote: ... the other half was placed first in 4% paraformaldehyde for 48 hr and then in a 30% sucrose solution for 48 hr at 4°C and cut into 30-µm-thick sections with a freezing microtome (Leica). For Extended Data Fig ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5% glutaraldehyde and 4% paraformaldehyde in buffer solution) removed and coronally cut into 350 µm thick sections using a Vibratome (Leica VT1000 S ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Neuroscience 2020Quote: ... the slices were embedded in 4% low melting agarose and cut in 70µm thick sections in a vibrating microtome (Leica, VT1000S). Sections around the tip of the recording electrode were collected and cryoprotected in 10% and 20% sucrose solutions in PB ...
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bioRxiv - Physiology 2021Quote: ... then individually embedded in paraffin blocks and sectioned in serial sagittal sections (4 μm) using a rotary microtome (Leica, Germany). Tissue sections were permanently mounted ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... with an Apochromat 63 x 1.4 NA oil-immersion objective and a charge-coupled device camera (ORCA-R2; Hamamatsu Photonics) using LAS-AF software (Leica). Color-coded time projections were generated in ImageJ using a publicly available plugin (K ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 20 µl of the protoplast suspension were pipetted to 200 µL high osmolarity solution (Supplementary Table 4) in a perfusion chamber mounted on the stage of an inverted microscope (DMi8, Leica). Protoplasts were allowed to settle down for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: HEK293 TauRDP301S-CFP/YFP and HEK293 TauRDP301S-CFP cells treated with Tau condensates were fixed with 4% PFA in PBS prior to confocal FLIM on a Stellaris 8 FALCON (Leica) equipped with a 100x objective ...
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bioRxiv - Genomics 2022Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Microbiology 2022Quote: ... the eyes were cut into 4 μm sections and stained with H&E (BBC Biochemical) using an autostainer (ST5010, Leica). Images were obtained using an Olympus BX51 microscope (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... images were taken as a time series on an xy plane across 6 z planes at an interval of 4 s using a 10× objective with an NA of 0.4 and an optical zoom of 4 on an SP5 inverted confocal microscope mounted with a resonant scanner (Leica Microsystems). The z project across time was then obtained as a time series.
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bioRxiv - Neuroscience 2020Quote: ... One hemisphere was post-fixed by incubation for 48 h in 4% paraformaldehyde (PFA) and cut into 35 μm sections with a vibratome (Leica) for histological analyses (brains used for RNAscope were perfused with both PBS and 4% PFA prior post-fixation and cut in 10 μm sections with a cryostat) ...
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bioRxiv - Cell Biology 2021Quote: ... brains fixed in 4% paraformaldehyde (PFA) were embedded in paraffin and sectioned 5µm thick using a microtome system (Leica RM2165). Immunohistochemistry was performed on brain sections by permeabilizing them with TBS-0.5% Triton-X (TBS-Tx ...
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bioRxiv - Physiology 2021Quote: ... tissue was fixed in 4% buffered paraformaldehyde and samples subsequently paraffin-embedded and 5 μm sections prepared on a microtome (Leica). Adipose sections were viewed at 20 x magnification ...
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bioRxiv - Cancer Biology 2020Quote: Paraffin tissue-blocks were serially sectioned with slices thickness of 4 µm on a microtome (Leica Byosystem, Buffalo Grove, IL) and allowed to dry at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed in 4% PFA overnight and coronally sectioned at 75 µm on a Vibratome (Leica VT1000 S). All slices were then mounted on Superfrost Plus slides using Fluoromount-G mounting medium (Southern Biotech ...