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Citations for Leica :
51 - 100 of 2029 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Evolutionary Biology 2024Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5-µm thick sections using a microtome (RM2155, Leica, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin (HE ...
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bioRxiv - Neuroscience 2023Quote: ... paraffin blocks were then sliced in coronal orientation at a thickness of 4-5 µm on a semiautomated rotary microtome (Leica, Germany). These sections were then collected ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA for 48 h at 4 °C and cut into 60 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... post-fixed in 4% PFA for 48 h at 4°C and cut into 100 μm coronal sections using a vibratome (VT1200S, Leica, Germany). The sections were post-fixed (1 h) ...
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bioRxiv - Neuroscience 2024Quote: ... Extracted brains were then stored in 4% PFA overnight at 4 before being sliced at 60 µm using a vibratome (Leica VT1000S). PVN and rPa regions were identified via the Allen Brain Atlas ...
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bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
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bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cancer Biology 2024Quote: ... immunostaining of formalin-fixed paraffin-embedded sections (2-4 μm) was performed with the Bond RX automated stainer (Leica Biosystems, Germany). Antigen retrieval was 20 mins at 100°C using the Bond Epitope Retrieval solution 2 (EDTA based buffer ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S, Leica, Wetzlar, Germany) in 50 µm slices ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The paraffin blocks were polymerized at 4°C and cut into 5 µm thick sections using a microtome (RM2155: Leica, Wetzlar, Germany). The sections were mounted on microscope slides coated with egg white-glycerin and stained using Delafield’s Hematoxylin and Eosin staining ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Molecular Biology 2023Quote: ... then cryoprotected with 20% sucrose in PBS for 24 hours at 4°C and cut in 5-µm-consecutive thick longitudinal sections using a freezing sliding microtome (Leica 2000R, Germany). Interstitial fibrosis was assessed via Picro Sirius Red Stain Kit (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... sectioned (4 um) using a cryostat CM1850 (Leica, CA), mounted onto microscope slides ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2024Quote: ... and Ultrostain-2 3% Lead citrate (Leica Microsystems#16707235). Images acquisition was done with JEOL JEM-1230 transmission electron microscope ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Cancer Biology 2024Quote: Harvested organs were incubated overnight in 4% paraformaldehyde (PFA) at 4°C and processed with the Leica Tissue Processor ASP-300-S (Leica Biosystems, Milton Kenes, UK). The tissues were incubated in formalin ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μm sections were cut on a microtome (Leica, Germany). Mounted sections on to charged slides and dried 2 hours at 62℃ ...
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bioRxiv - Pathology 2021Quote: ... staining the microtome sections obtained (4 µm: Leica® 2155) with hematoxylin-eosin [36,37].
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bioRxiv - Neuroscience 2022Quote: Photomicrographs were captured with a fluorescent microscope (Leica DM-4) with a 20x objective (80x final magnification) ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections of 4 µm were cut (Leica RM2155 microtome) and stained with hematoxylin and eosin (HE ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS at 4°C until vibratome (VT 1000S, Leica) cutting (30 μm coronal midbrain sections) ...
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bioRxiv - Biophysics 2023Quote: ... for 4 s using a Leica EM GP2 (Leica microsystems) with the chamber set to 30°C and 90% humidity and subsequently plunged into liquid ethane.
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bioRxiv - Neuroscience 2024Quote: ... Photomicrographs were captured with a fluorescent microscope (Leica DM-4) with a 20x objective (80x final magnification) ...
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bioRxiv - Neuroscience 2021Quote: ... Z-stack images of 1 µm interval (total depth of 4 µm) were processed with LAS software (Leica) and analyzed with Fiji software ...
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bioRxiv - Cell Biology 2020Quote: ... SSCs were incubated with the labeled EVs for 1–4 h and visualized using fluorescent microscopy (Leica, Wetzlar, Germany)
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
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bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).