Labshake search
Citations for Leica :
901 - 950 of 1261 citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
-
bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S ...
-
bioRxiv - Microbiology 2020Quote: ... After immunolabelling the grids were embedded with a mixture of 50% methylcellulose 2% and 50% sucrose 2.3M and then vitrified by plunge freezing with EMGP plunge freezer (Leica) at 30 °C and 90% humidity.
-
bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S ...
-
bioRxiv - Biochemistry 2022Quote: ... The ibidi dishes were mounted for time-lapse microscopy 2 h after induction on a Delta Vision microscope (Leica). Mitotic progression was monitored by taking images every 5 min for 20 h at different areas of the wells and three z-stacks (5 μm apart) ...
-
bioRxiv - Neuroscience 2022Quote: ... At least 3 different fields from 2 slices per experiment were analyzed by using LAS AF Lite software (Leica). The fluorescence signal corresponding to the PLP-DsRed OLs was quantified by ImageJ software and data were expressed as arbitrary units of fluorescence for each experimental situation ...
-
bioRxiv - Developmental Biology 2021Quote: ... and photographed with an Aperio AT 2 Leica Scanner and an Aperio Scan Scope Console program (Leica, Wetzlar, Germany) available at the Northern Finland Biobank Borealis ...
-
bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
-
bioRxiv - Pathology 2022Quote: ... Super-resolution images were recorded on the confocal laser scanning microscopy LSM 980 equipped with Airyscan 2 (Leica, Germany).
-
bioRxiv - Cancer Biology 2022Quote: ... using the following conditions: antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation with anti-P53 ...
-
bioRxiv - Developmental Biology 2020Quote: Embryos or neural tube explants are fixed in 3.7% formaldehyde for 2 hours and sliced using a vibratome (Leica). Proteins were detected on 50 μm vibratome sections ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections mounted on Pioloform filmed copper grids prior to staining with 2% aqueous uranyl acetate and lead citrate (Leica). Ultrathin sections were examined on a Phillips CM 100 Compustage (FEI ...
-
CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Collected aortas were placed between 2 clear sheets and imaged by M205 FA stereoscope system with Cy5 channel (Leica). CXCR4 co-staining was done after fixation using whole mount staining protocol described above.
-
bioRxiv - Cell Biology 2019Quote: ... Cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and imaged with a Leica TCS SP5 Confocal Laser Scanning Microscope (Leica) using a 40X water immersion objective.
-
bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
-
bioRxiv - Cell Biology 2020Quote: ... then transferred to brass specimen carriers and high pressure frozen with an EMPACT 2 (Leica Biosystems, Buffalo Grove, IL).
-
bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
-
bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 2 to 3 days after infiltration using a SP8 laser scanning microscope (Leica Microsystems GmbH) with LAS AF and SymPhoTime (PicoQuant ...
-
bioRxiv - Neuroscience 2023Quote: ... and MgCl2 7) for 2 minutes and subsequently sliced in the same solution using a vibrating microtome (Leica VT1200S). Slices were allowed to recover for 1 hour at 33°C in carbongenated standard aCSF which contained (mM) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The standard ACD Red Rev BOND Rx protocol was modified to include BOND Epitope Retrieval Solution 2 (Leica Biosystems) pretreatment for 25min at 95°C and to increase Amp5 step time to 30 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... Slides were imaged on an inverted Leica TCS SP5 confocal microscope using a 63x/1.40 oil objective with 2-3x digital zoom at calibrated magnifications and recorded with LAS AF software (Leica). Quantitation of dextran and LAMP-1 colocalization with bacteria was analyzed with CellProfiler 3.1.9 (27 ...
-
bioRxiv - Cell Biology 2022Quote: ... fluorescence images were captured every 2 s for 6 min using fluorescence microscopy (#AF6000-DMI6B; Leica Microsystems, Wetzlar, Germany) (objective lens ...
-
bioRxiv - Biochemistry 2022Quote: ... Plunging was performed at 90-100% humidity via automatic backside blotting (via Leica Microsystems Cryo GP 2 plunge freezer) or dual-side blotting (Mark IV FEI/Thermo Fisher Scientific Vitrobot) ...
-
bioRxiv - Developmental Biology 2023Quote: ... outside of the necrotic region) (Figure 8-figure supplement 1E-F) from 2 sections with the Application Suite Core v2.4 software (Leica).
-
bioRxiv - Physiology 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 95 °C for 5 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
-
bioRxiv - Genomics 2020Quote: ... Peroxide block (Bond Polymer Refine Detection Kit [DS9800], Leica Biosystems) was applied for blocking the tissue endogenous peroxidase activity for 15 mins ...
-
bioRxiv - Cancer Biology 2019Quote: ... using the bond polymer refine detection kit (DS9800, Leica Biosystems). The antibody was incubated for 1 hour at a concentration of 1:100 ...
-
bioRxiv - Genomics 2021Quote: ... using the Bond Polymer Refine Detection Kit (DS9800; Leica Biosystems). Sections were first applied to a peroxide blocking step for epitope retrieval using Epitope Retrieval Solution I and II (AR9640 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Staining was completed with BOND Polymer Refine Detection kit (Leica) including DAB chromogen and hematoxylin nuclear stain ...
-
bioRxiv - Cancer Biology 2022Quote: ... using the Novolink Polymer Detection kit (Leica, Cat# RE7140-CE). Cells were counterstained with haematoxylin (Reagecon ...
-
bioRxiv - Plant Biology 2022Quote: ... and infiltrated using a HistoResin embedding kit (Leica, www.leica-microsystems.com), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the Bond Polymer Refine Detection kit (DS9800, Leica Biosystems). Positive and negative tissue controls and isotype controls were used for examining the specificity of staining and species cross-reactivity for both PHOX2B and COL1A1 in comparison with the experimental tissue slides ...
-
bioRxiv - Cell Biology 2023Quote: ... Bond Refine Detection Kit (Leica Biosystems, Newcastle Upon Tyne, UK), and Opal 6-Plex Detection Kit for Whole Slide Imaging (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2023Quote: ... Using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) as described (52) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Visualization used a Bond Polymer Refine Detection Kit (Leica, #DS9800) (RRID:AB_2891238 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and BOND IHC Polymer Detection Kit (Leica Biosystems, cat# DS9800) was used to stain the antibody with DAB Cromogen ...
-
bioRxiv - Neuroscience 2021Quote: Acute mouse coronal slices containing hippocampus region (250 μm) were prepared from anesthetic mice using a vibrating tissue slicer (Leica VT1000S) in ice-cold artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Genomics 2021Quote: ... primary antibody was diluted 1:10 in the peptide solution directly.) The primary antibodies were detected by 30 minute incubation with HRP-labeled anti-mouse secondary antibody (catalog# PV6114, Leica Microsystems) followed by detection with 3,3′-Diaminobenzidine (catalog# D4293 ...
-
bioRxiv - Evolutionary Biology 2021Quote: Embryonic bat heads and embryonic E16.5 mouse heads were submerged in chilled lx PBS and imaged using a stereo microscope with brightfield illumination (Leica M165 FC) in standard anatomical views ...
-
bioRxiv - Neuroscience 2021Quote: ... and −1.46 mm (dorsal hippocampus) from bregma according to the Paxinos and Franklin mouse brain atlas54 in triplicate using a cryostat (Leica, Germany) and dry mounted on coated Superfrost Plus slides (Thermo Fischer Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
-
bioRxiv - Neuroscience 2021Quote: Embryonic left and right hippocampus was microdissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in Lepagnol-Bestel et al ...
-
bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
-
bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
-
bioRxiv - Immunology 2020Quote: ... Non-invasive intravital imaging of mouse ear was performed using Leica DIVE (Deep In Vivo Explorer) inverted confocal microscope (Leica Microsystems) with full range of visible lasers (Spectra Physics) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... using microhematocrit capillary tubes (10 μL) and measured the hematocrit rate for each mouse using a Critocaps Micro-Hematocrit Capillary Tube Reader (Leica Microsystems) (22).