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Citations for Leica :
801 - 850 of 1261 citations for Mouse Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Developmental Biology 2021Quote: High-pressure frozen samples were vacuumed to 5 × 10−7 mbar at -120 °C and a fracture was conducted at the interface between the two HPF discs containing the sample (BAF 60, Leica Micro-systems, Vienna, Austria). One disc containing the exposed cryo-fixed ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Plants were watered and left at ambient conditions (24 °C) with lid on top and imaged two days past transformation with an SP8 confocal laser scanning microscope (CLSM) (Leica Microsystems GmbH, Wetzlar, Germany) with LAS AF and SymPhoTime software (Picoquant GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Cell Biology 2022Quote: ... The plates containing the pseudostratified epithelia were incubated at 37 °C with 5% CO2 in the 3i Marianis/Yokogawa Spinning-Disk Confocal microscope (Leica Microsystems, Sugar land, USA) as reported in our previous publications (53 ...
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bioRxiv - Biophysics 2022Quote: ... Confocal images and super-resolution images (Figure 3A, C, D; Figure S2A) were acquired under 100× objectives on a STED confocal microscope (Leica TCS SP8 STED 3X). Images (Figure S2C ...
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bioRxiv - Microbiology 2021Quote: ... pre-warmed at 37°C and the channels were imaged at x64 magnification using a DM-IRE2 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Germany) and the accompanying Leica Microsystems Confocal Software (version 2.61 Build 1537) ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S, Leica Microsystems Nussloch GmbH, Germany). To separate the organic shields ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2, Leica Microsystems GmbH, Wetzlar, Germany). For 48 hours ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... the discs were loaded into a Leica ICE high pressure freezing machine and vitrified at liquid nitrogen temperature (−192 °C) at 210 MPa (Leica Microsystems GmbH, Wetzlar, Germany). Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2 ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Epon-embedded samples were cured at 70 °C for 72 and then sectioned to 70 nm thin sections using Ultracut UCT ultra-microtome (Leica Microsystems GmbH, Wetzlar, Germany) equipped with an Ultra 45° diamond knife (Diatome Ltd ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was incubated for 30 s at 90 % relative humidity and 16°C inside Leica EM ACE 600 (Leica EM GP2 plunge freezer), blotted for 1.2 s and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S, Leica Biosystems GmbH, Wetzlar, Germany). Brain slices containing hippocampal areas of all three animals were deposited on the same slide ...
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bioRxiv - Cell Biology 2023Quote: ... for 4 hours and followed by Lowicryl HM20 for 24 hours with agitation and polymerized under UV exposure at −45 °C in an automated processor (AFS2, Leica Microsystem Inc., Vienna, Austria). Ultrathin sections (90 nm thickness ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were cast into molds together with liquid paraffin and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Developmental Biology 2022Quote: Images from mouse and human samples were acquired with a Leica TCS SP8 confocal laser scanning microscope (Leica Microsystems). All images are shown as maximum intensity projections of acquired z-stacks of about one cell thickness (6-10 µm) ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 μm transverse zebrafish and mouse cryosections were collected on a Leica CM1900 crysostat (Leica Biosystems, Buffalo Grove, IL). 10 μm paraffin mouse sections were collected on a Thermo Scientific Shandon Finesse ME Microtome (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... transverse slices of 150µm from the hippocampi of a P17 mouse were prepared using a vibrating blade microtome (Leica) in cold (4°C ...
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bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Microbiology 2023Quote: ... FFPE mouse skin was processed and cut onto a polyethylene naphthalate (PEN) slide designed for LCM processing (Leica 11505158). At least 1,000 SGs or 1,000,000 μm2 of tissue was isolated to obtain enough material for RNA extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Neuroscience 2023Quote: ... The mouse brains were then cut into 20 µm thick frozen tissue sections using a cryostat (Leica, Cat# CM1950) and placed on Superfrost Plus Adhesion Microscopic glass slides (Epredia ...
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bioRxiv - Microbiology 2022Quote: ... BondTM Polymer Refine Detection visualisation kit (Leica Byosystems, Germany) was afterwards used for signal detection using DAB as chromogen and counterstaining with hematoxylin.
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bioRxiv - Immunology 2022Quote: ... The revelation system (“Bond Polymere Refine” kit, DS9800, Leica) included a secondary antibody HRP (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... the BOND Polymer Refine Detection Kit (Leica Biosystems, UK) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... and the BOND Polymer Refine Detection kit (Leica Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...