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Citations for Leica :
801 - 850 of 2056 citations for N 2 Chloro 5 1 oxo 2 3 pentadecylphenoxy butyl amino phenyl 4 4 dimethyl 3 oxovaleramide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... images were captured every 2 h for 72 h by the IncuCyte Live-Cell analysis system (Leica).
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bioRxiv - Developmental Biology 2023Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in a FS system (AFS-2, Leica Microsystems). Larvae were embedded in Epon-Araldite mix (EMS hard formula) ...
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bioRxiv - Immunology 2022Quote: ... Paraffin embedded kidney sections (2 µm) were cut on a Leica RM 2165 microtome (Leica, Wetzlar, Germany) and dried at 60°C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitope Retrieval Solution 2, Leica Biosystems, cat# AR9640).
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bioRxiv - Bioengineering 2022Quote: ... and counted for expression of opie-2-dsRed and 3xP3-tdTomato using a fluorescent stereoscope (Leica M165FC). The difference in total larval counts compared to total egg counts were considered to be dead during embryonic or early larval stages ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05% uranyl acetate in acetone and placed in an AFS-2 Freeze-Substitution Machine (Leica Microsystems, Vienna). Samples were freeze-substituted at -90°C for 72 h ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% glutaraldehyde and 0.25% uranyl acetate for 60 h in an FS system (AFS-2; Leica Microsystems). The embryos were embedded in an Epon-Araldite mix (EMS hard formula) ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... 2 μm thin slides were then prepared and immunohistochemistry was performed on a Bond Rxm system (Leica) with primary antibodies against Hexokinase 1 (1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... Semi thin (2 μm) and ultra-thin (70 nm) sections were cut with an ultra-microtome (Leica Ultracut ...
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bioRxiv - Immunology 2024Quote: ... Further submersion in 2% agarose allowed preparation of 200μm Vibratome sections (Leica VT1000 S Vibrating blade microtome). mTOs (or sections ...
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bioRxiv - Synthetic Biology 2024Quote: ... Confocal microscopy was performed by immobilizing samples on 2% agarose pads (Fisher; BP1356-500) using SP8 (Leica). Images were acquired and processed with Leica Application Suite software ...
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bioRxiv - Biochemistry 2024Quote: ... Grids were vitrified in 100% liquid ethane using a Leica EM GP 2 plunge freezer (Leica Microsystems) with a blot time of 1 s or 3 s ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Artemia individuals (n = 47) were observed and photographed (Leica EZ4HD stereomicroscope with integrated HD camera ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
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bioRxiv - Cancer Biology 2021Quote: Larvae fixed in 4% PFA overnight at 4°C were embedded in 4% low melting temperature agarose and transverse sections collected at 200 µm intervals using a vibrating microtome (Leica VT 1000S). Sections were blocked with 1% BSA in PBS/0.3% Triton X-100 and incubated with a 1:750 dilution of mAb414 (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections from V1 were mounted on gelatin-coated slides and cover-slipped with Prolong Diamond Antifade Mounting Media (P36965 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were post-fixed for 24 hours and then included in agar 4% and serial cuts of 40 µm thick were made using vibratome (Leica Biosystems, S2000).
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bioRxiv - Cancer Biology 2021Quote: ... The specimens were embedded in paraffin blocks and serial sections of 4 μm thickness were cut using a microtome (Leica Biosystems, Germany). The sections were stained by Hematoxylin & Eosin (H&E)34 Histological photographs were obtained using a digital light microscope (Olympus ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were postfixed in 4 % PFA for 24 hours until they were sliced (70 µm thickness) using a Leica Vibratome (Leica, Wetzlar, Germany). Slices were directly mounted on an object slide (Gerhard Menzel ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated in 10% and then 30% sucrose in PBS for 12-24 hours at 4 °C before being cut into 50 µm sections by a freezing-sliding microtome (Leica SM 2101R). Sections with LGN and V1 were then mounted on gelatin-coated slides and cover-slipped with mounting media (Prolong Diamond Antifade Mounting Media ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Microbiology 2022Quote: ... Grids were sputter-coated with a ∼4 nm thick layer of tungsten using ACE600 cryo-sputter coater (Leica Microsystems GmbH, Vienna, Austria) and afterwards transferred into a Crossbeam 550 FIB-SEM dual-beam instrument (Carl Zeiss Microscopy ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were post-fixed overnight in 4% PFA and stored in PBS until histological slicing was performed using a vibratome (Leica VT1200 S) and the slices were mounted in DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Developmental Biology 2022Quote: ... Electroporated hemispheres were infiltrated with 30% sucrose at 4°C overnight prior to embedding in TissueTek tissue freezing medium (Leica Biosystems, Germany). 16 µm-thick sections were cut using a cryostat (Leica Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Formalin-fixed paraffin-embedded (FFPE) samples were cut to a thickness of 4 μm and stained on an automated stainer (BOND-MAX, Leica Biosystems, Germany) as part of the diagnostic routine in a clinically accredited histology lab ...
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bioRxiv - Immunology 2020Quote: ... at which time tissues were removed from the BSL-3 laboratory and stored in 1X PBS at 4°C until processed in a Leica PELORIS automated vacuum infiltration processor (Leica, Wetzlar, Germany), followed by paraffin embedding with a Histocore Arcadia paraffin embedding machine (Leica) ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Ribbons of 150-200 serial sections of thicknesses of approximately 55nm were cut with 4 drops of pure ethanol in the water boat and an ionizing instrument (Leica EM Crion) activated and oriented towards the cutting edge of the knife from above ...
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bioRxiv - Neuroscience 2023Quote: ... Brains not used for two-photon imaging were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 µm coronal (S1 injections) or parasagittal (SNc injections) sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% paraformaldehyde in PBS on a shaker at 4°C and cut into 50 μm coronal sections on a vibrating microtome (Leica, VT-1000S). Sections were collected sequentially into 6 tubes containing cryoprotectant ...
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bioRxiv - Developmental Biology 2023Quote: ... The oocytes were fixed in 4% PFA/PBS for 30 min and observed using the Leica M205FA stereomicroscope (Leica Microsystems, Wetzlar, Germany) or FV3000 Confocal Microscope.
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bioRxiv - Bioengineering 2024Quote: ... The fixed constructs were embedded in low-melting point agarose 4% w/v in pre-warmed PBS and sliced to a thickness of 300 μm with a vibratome (Leica Biosystems, VT1000S) filled with PBS 1x ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Bioengineering 2024Quote: ... and fetal pig kidneys were fixed with 4% paraformaldehyde (FUJIFILM Wako Pure Chemical Corporation) and sectioned with a cryostat (Leica Microsystems GmbH) to prepare frozen sections ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were cryoprotected in 30% sucrose/PBS on a rotor shaker overnight at 4°C and sectioned at 20 µm on a cryostat (Leica 1850 UV). Sections were permeabilized in 0,3% Triton X-100/PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were embedded in 4% oxidized agarose as previously described (Ragan et al., 2012) to limit artifacts during sectioning on a vibratome (Leica VT1000 S). For 20 μm cryotome sectioning ...
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bioRxiv - Immunology 2024Quote: ... The brains were quickly dissected and fixed in 4% paraformaldehyde (PFA) overnight and then cut into 30 µm sections using a vibrating microtome (Leica, VTS1200, Germany). For immunostaining ...
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bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
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bioRxiv - Neuroscience 2024Quote: Hemibrains were drop-fixed in 4% paraformaldehyde for two days and sectioned at 40 mm on a vibratome (Leica, Buffalo Grove, IL). Sections were collected in a 1 in 4 series and stored in PBS at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Microbiology 2024Quote: ... sucrose in PBS overnight at 4°C and embedded in OCT compound (Agar Scientific) and cryosectioned (10 µm thick, Leica CM1850 Cryostat). Sections were dried overnight onto Thermo Scientific SuperFrost Plus Adhesion slides ...
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bioRxiv - Developmental Biology 2024Quote: ... The tissues were then embedded in paraffin wax and cut into 4-μm sections with a microtome (RM 2165; Leica Microsystems GmbH). Sections were stained with haematoxylin and hemoglobin (H&E) ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Novolink DAB Substrate Buffer (Leica Biosystems, 1:20, 5 min). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... 0.05% uranyl acetate in acetone and then placed in a AFS-2 freeze substitution machine (Leica Microsystems, Vienna). Samples were freeze substituted at −90°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then cut into sections of 2 μm thickness with an ultra-microtome (Leica EM UC6). Sections were placed on glass coverslips for immunohistochemistry.
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal cerebellum sections 300 μm thick were cut with a vibratome (VT1200S, Leica or 7000smz-2, Campden Instruments) in ice-cold high-sucrose ACSF ...
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bioRxiv - Neuroscience 2020Quote: Fluorescence images were acquired using a Leica SP5 Tandem Scanner Spectral 2-Photon scanning confocal microscope (Leica Microsystems), or Marianas Yokogawa type spinning disk confocal microscope with back-thinned EMCCD camera ...