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Citations for Leica :
1051 - 1100 of 2056 citations for N 2 Chloro 5 1 oxo 2 3 pentadecylphenoxy butyl amino phenyl 4 4 dimethyl 3 oxovaleramide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections (∼2 x 2 x 2cm) not needed for pathologic evaluation were fixed in 10% formalin and sliced with a vibratome (Leica VT 1200S) to a thickness of 2mm ...
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bioRxiv - Neuroscience 2023Quote: ... Stellaris 5 (Leica), or LSM 900 (Zeiss ...
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bioRxiv - Physiology 2024Quote: ... directing it to the infinity-corrected objective (Leica 2.5 N Plan, NA=0.7). The stirring mirror and the telescope were adjusted to achieve homogeneous illumination at the objective’s working distance ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Microbiology 2024Quote: ... It was outfitted with an oil immersion objective (Leica N PLAN 50x/0,90 OIL), a fiber-coupled mercury lamp ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Biophysics 2021Quote: N&B imaging was performed on a TCS SP8 confocal microscope (Leica Biosystems, Wetzlar, Germany) equipped with a HyD hybrid detector in photon counting mode ...
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bioRxiv - Molecular Biology 2022Quote: ... All images were taken no longer than 2 hrs after gavage using the wide-field feature of a LEICA DM6 confocal microscope (Leica Microsystems Inc., Buffalo Grove, IL).
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bioRxiv - Microbiology 2020Quote: ... Time-lapse imaging of the samples was performed in imaging medium (RPMI1640 without phenol red containing 10% FBS and 2 µg/ml lysostaphin) on a Leica TCS SP5 confocal microscope using a 40x (Leica HC PL APO, NA=1.3) or 63x (Leica HCX PL APO ...
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bioRxiv - Neuroscience 2020Quote: ... The slices of cochlear nucleus were cut at 220-300 μm in ice-cold sucrose solution on a vibratome (Leica VT1200S or Campden 7000smz-2). Slices were transferred into standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Biochemistry 2022Quote: ... and 380 nm (F380) and λem = 510 nm in a Leica DMi-8 microscope equipped with a Fura-2 filter (Leica Microsystems, Buffalo Grove, IL, USA). Cells were followed through additions of CGP (10 μM) ...
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bioRxiv - Neuroscience 2024Quote: High-pressure freezing and freeze-substitution (HPF-FS) were performed at Harvard Medical School using the Leica EM ICE and EM AFS-2 (Leica Microsystems Inc, Buffalo Grove, IL). Whole brains were immediately frozen upon dissection ...
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bioRxiv - Plant Biology 2023Quote: ... mature rice pollen grains were stained with 2 % I2-KI (w/v) solution and observed under a bright field microscope (Leica DM IL LED S-80) according to Chhun et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Resin blocks were trimmed to approximately 2 mm2 and sectioned at 70 nm using a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc., Buffalo Grove, IL) and a Diatome diamond knife (Electron Microscopy Sciences ...
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bioRxiv - Developmental Biology 2022Quote: ... and Stellaris 5 (Leica Microsystems) confocal microscopes.
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bioRxiv - Cell Biology 2020Quote: ... Sections were then washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and mounted on slides (Leica Surgipath® X-tra™ Adhesive, 3800202) using Vectashield Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal ultrathin sections (70 nm thick) were prepared using an ultramicrotome (Ultracut N ultramicrotome, Leica Microsystems) and stained with uranyl acetate for 5 min and Reynold’s lead citrate solution for 3 min ...
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bioRxiv - Microbiology 2023Quote: ... Ultrathin sections were prepared using a Porter Blum ultramicrotome (Reichert-Nissei ULTRACUT-N; Leica, Wetzlar, Germany) and mounted on a copper grid (200 mesh ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Neuroscience 2021Quote: ... with immersion oil conforming to ISO 8036 with a diffraction index of n=1.5180 (Leica Microsystems, Germany). For gSTED imaging ...
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bioRxiv - Neuroscience 2021Quote: ... with immersion oil conforming to ISO 8036 with a diffraction index of n=1.5180 (Leica Microsystems, Germany). Emitted light was detected with two HyD detectors in photon counting mode (Leica Microsystems ...
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bioRxiv - Plant Biology 2023Quote: ... Leica DMI6000B equipped with a Leica N Plan L 20×/0.4 CORR PH1 objective (Leica, Wetzlar, Germany) and a Hamamatsu Orca camera (model C4742-80-12AG ...
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bioRxiv - Developmental Biology 2023Quote: ... used for CBs and a x10 objective (N PLAN 10x/0.25 achromatic corrected objective, Leica Microsystems CMS) used for OZ and AM ...
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bioRxiv - Genomics 2020Quote: ... Microtome sections (5 μm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Cell Biology 2024Quote: ... Microtome sections (5 µm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Developmental Biology 2021Quote: ... Water immersion 10× objectives were used in both systems: N-Achroplan 10×/0.30 NA (Zeiss) and HC APO L 10×/0.30 NA (Leica), respectively ...
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bioRxiv - Molecular Biology 2020Quote: Laser powers used were measured behind the objective lens (N PLAN L 20s/0.40, Leica Microsystems, Wetzlar, Germany) with a LabMax-TO laser power meter equipped with an LM-2 VIS sensor (Coherent ...
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bioRxiv - Cancer Biology 2024Quote: ... dried at 37°C O/N and then processed with Bond-RX fully automated stainer system (Leica Biosystems) according to the following protocol ...
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bioRxiv - Genetics 2023Quote: ... Longitudinal microtome sections (5 µm, Leica RM2245) were mounted on adhesive slides (Klinipath-KP-PRINTER ADHESIVES) ...
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bioRxiv - Physiology 2023Quote: ... The 5-µm tissue sections (Leica CM1950) embedded with paraffin were dewaxed in xylene and rehydration ...
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bioRxiv - Developmental Biology 2023Quote: ... or a Stellaris 5 confocal microscope (Leica) or two Spinning disk Confocals (3i and DragonFly from Andor).
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bioRxiv - Cell Biology 2023Quote: ... or a confocal microscope (Leica Stellaris 5) with a 63 × (1.4 NA ...