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Citations for Leica :
701 - 725 of 725 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The tissues were covered with the flat side of a Type-B brass planchette and rapidly frozen with an HPM-010 high-pressure freezing machine (Leica Microsystems, Vienna Austria). The frozen samples were transferred under liquid nitrogen to cryotubes (Nunc ...
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bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
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bioRxiv - Neuroscience 2021Quote: ... Regularly spaced brain series (5 × 1-in-5) of 30 μm-thick frozen sections were cut in freezing microtome (SM2000R, Leica, Wetzlar, HE, Germany) in the coronal plane ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 3 μl were added to the plasma cleaned grids and subsequently plunge-frozen in liquid ethane using a Leica EMGP (Leica microsystems, Wetzlar, Germany). Blotting time was set to 1s with the chamber temperature and humidity being at 20°C and 85% ...
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bioRxiv - Microbiology 2022Quote: ... back-blotted for 6-8 s after a 30-s pre-blotting time and immediately plunge frozen into liquid ethane using a GP-EM2 (Leica Microsystems, Wetzlar, Germany) with a chamber maintained at 20 °C and 95% humidity ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen and coronally sectioned at a thickness of 30 μm (1:5 series) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Fos2A-iCreER+/-::Ai14+/- sections were mounted on gelatin-coated slides and coverslipped with Vectashield containing DAPI (cat ...
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bioRxiv - Biochemistry 2023Quote: The high-pressure frozen samples were mounted on a holder and transferred to a BAF 60 freeze-fracture device (Leica Microsystems, Vienna, Austria) using a VCT 100 Vacuum Cryo Transfer device (Leica) ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then frozen rapidly on dry ice before being coronally sectioned into 100 μm sections using a Leica CM3050 cryostat (Leica Biosystems, Richmond, IL). Slices were placed in 0.01M PBS and the ones containing the site of infection were selected to be stained ...
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bioRxiv - Neuroscience 2023Quote: ... The brainstem areas containing the DVC and BAT sections were put in 15% followed by 30% sucrose until saturated and frozen in a cryo-embedding medium (Leica Biosystems, Nussloch, Germany). Brain sections were cut at 30 µm and BAT sections were cut at 5 µm using a cryostat (Leica Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... elegans embryos were collected in cellulose capillary tubes (Pelletier et al., 2006) and high-pressure frozen as described using an EM ICE high-pressure freezer (Leica Microsystems, Vienna, Austria) (Muller-Reichert et al. ...
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bioRxiv - Cell Biology 2023Quote: ... On day 1 after seeding the grids were incubated with 10 nm colloidal gold particles for 1 minute and rapidly frozen in liquid ethane using GP2 plunge-freezer (Leica Microsystems, Vienna, Austria). Tilt series of vitrified samples were collected using a Tecnai F20 microscope (FEI Company ...
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bioRxiv - Cell Biology 2023Quote: ... elegans embryos were collected in cellulose capillary tubes(44) and high-pressure frozen as described using an EM ICE high-pressure freezer (Leica Microsystems, Vienna, Austria)(45) ...
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bioRxiv - Neuroscience 2023Quote: ... All brains were then flash frozen in OCT solution and cut at 100um thickness using a cryostat (Leica CM 1950) (Leica Biosystems, Illinois/USA). Pyramidal neurons in dorsal CA1 region of the hippocampus ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Centrifuged cells were immersed in a cryoprotectant (20% BSA in cell medium) and immediately frozen with a Leica HPM100 high-pressure freezer (Leica Microsystems, Vienna, Austria). The cells were freeze substituted in 2% OsO4 and 100% acetone using an automatic freeze substitution system Leica EM AFS2 ...
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bioRxiv - Bioengineering 2019Quote: ... United States) and both 150-μ;m and 10-μ;m frozen sections were collected via the Leica CM350 Cryostat (Leica Microsystems, Wetzlar, Germany). The 150-μ;m sections were plated on glass slides and FLI images were collected using the IVIS Lumina XRMS In Vivo Imaging System (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Physiology 2021Quote: Frozen forebrains of DIO/DR generation F7 were sectioned in four series of 25 μm thickness on a cryostat (Leica CM3050 S; Biosystems; DE). Sections were mounted directly onto Superfrost® glass slides (Superfrost® Plus ...
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bioRxiv - Neuroscience 2022Quote: ... The cryoprotected blocks were then flash-frozen using dry ice and sectioned at 40 μm using a Leica SM2000R sliding microtome (Leica Biosystems, Deer Park, IL). For immunohistochemistry ...
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bioRxiv - Neuroscience 2022Quote: ... The brain was mounted on the vibratome stage using adhesive and placed in the slicing chamber submerged in partially frozen modified aCSF (Leica VT1000S, Leica Microsystems, Germany). 300um coronal sections were taken along the extent of the MGN (AP ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μl was transferred into an HPF flat specimen carrier and frozen with a Leica EM PACT high-pressure freezer (Leica Microsystems, Inc., Bannockburn, IL) at a typical rate of 1,700°C/s ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Neuroscience 2022Quote: ... 10 control and 10 MK801-treated brains were cut to 10-μm thick frozen sections with a freezing microtome (Leica CM 1860 UV, Leica, Germany). The sections were stained with hematoxylin and eosin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were flash frozen in a slurry of dry ice and isopentane and sectioned on a cryostat (Leica CM 1860 Leica Biosystems, Buffalo Grove, IL, USA).