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Citations for Leica :
651 - 700 of 725 citations for Bordetella Pertussis Toxin Frozen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The tissue samples were frozen in OCT (Tissue-Tek) on dry ice and sectioned at 10 μm on a cryostat microtome (Leica CM1950). Tissue slides sectioned from the anterior of the hippocampus containing typical dentate gyrus structures were first incubated in blocking and permeation solution with 2% Triton X- 100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... samples were high-pressure frozen using the High Pressure Freezer Leica EM ICE equipped with the Coral Life workflow (Leica Microsystems). It took 1.0‒1.5 minutes from the last time-lapse imaging until the high-pressure freezing ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were pelleted by centrifugation at ∼800 RCF for five minutes and high-pressure frozen in the 200-µm recess of an aluminum platelet (Engineering Office M. Wohlwend 241) using an HPM100 (Leica Microsystems). The samples were then freeze substituted in 0.1% uranyl acetate ...
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bioRxiv - Neuroscience 2024Quote: ... brains were immediately frozen and stored at −80°C until sectioning at 12 μm on a cryostat (Leica Biosystems, Wetzlar, DE). PVH and ARC tissue was collected from brain sections using laser capture microdissection with a PALM Robot Microbean (Carl-Zeiss ...
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bioRxiv - Immunology 2023Quote: ... and a slice of the brain containing the tumor was included in OCT (FSC22 blue frozen section compound, Leica Biosystems, 3801480). Sectioning was performed using the Agora histology platform and the sections were stored at −80°C ...
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bioRxiv - Cell Biology 2020Quote: ... were quick-frozen by high-pressure freezing using an HPM 010 high-pressure freezing machine according to the manufacture’s instruction (Leica Microsystems, Wetzlar, Germany). The frozen specimens were transferred to the cold stage of a Balzers BAF 400 apparatus and fractured at −115° to −105°C under vacuum at ~ 1 × 10−6 mbar.
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bioRxiv - Developmental Biology 2021Quote: ... Gelatine blocks were flash frozen at −80 °C in pre-cooled heptane and sectioned in 25 μm slices in a cryostat (Leica, CM3050 S). The slides dried overnight at room temperature and were mounted with mowiol and sealed with nail polish prior to confocal microscopy.
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bioRxiv - Neuroscience 2019Quote: ... frozen using dry ice and subsequently sectioned in the coronal plane (40 µm) using a cryostat (Leica Biosystems Inc, Buffalo Grove, IL). For Experiment 1 ...
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bioRxiv - Physiology 2019Quote: ... serial cross-sections (sections cut after on another) (7 μm) were obtained from frozen samples using a microtome (Leica Microsystems, Wetzlar, Germany). The sections were thaw-mounted on Polysine glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... tissue samples were transferred into gold or copper carriers and frozen under high pressure (>300 bar) using an HPM010 (Leica, Wetzlar, Germany). Frozen samples were stored in liquid nitrogen until further processing ...
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bioRxiv - Biophysics 2021Quote: ... The grids with live cells on them were then blotted and plunge frozen in liquid nitrogen cooled liquid ethane (Leica EM GP2) before being transferred to liquid nitrogen storage ...
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Genetics 2022Quote: ... Tissue was then frozen at -20 °C for at least 2 hrs and sectioned (50–100 μm sections) on a cryostat (Leica CM3050 S) and mounted on glass slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Neuroscience 2020Quote: ... brains were rapidly frozen in isopentane and dry ice and cut at 100-μm thick sections in a cryostat (Cryocut 1800, Leica, Heerbrugg- Switzerland) to obtain slices containing the hippocampus ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were plunge frozen in liquid nitrogen and subsequently placed in a cryo-preparation chamber (MED 020/VCT 100, Leica, Vienna, Austria). To sublimate any water vapor contamination (ice ...
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bioRxiv - Developmental Biology 2021Quote: ... The sucrose infiltrated Ring gland in gelatin was mounted on an aluminum pin and snap frozen in LN2 and cryosectioned on a Leica Ultracut EM UC6 ultramicrotome (Leica Microscopy systems). 70nm Sections were picked up using a 1 :1 mixture of 2.3M sucrose and 2% methyl cellulose (cat# M-6385 ...
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bioRxiv - Cell Biology 2020Quote: ... The grids with live cells on them were then blotted and plunge frozen in liquid nitrogen cooled liquid ethane (Leica EM GP2) before being transferred to liquid nitrogen storage ...
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bioRxiv - Cell Biology 2020Quote: ... The grids were manually blotted using Whatman filter paper and plunge-frozen into liquid ethane at −174°C using a Leica EM-CPC equipment (Leica, Wetzlar, Germany). After freezing ...
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bioRxiv - Immunology 2022Quote: ... The frozen SCEM samples were then cut in the sagittal plane with a disposable tungsten carbide steel blade (Leica Microsystems, Nussloch, Germany) using a microtome (CM3050sIV ...
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bioRxiv - Biochemistry 2022Quote: ... 1 at 10 °C and maintained at 90% humidity and then plunge frozen into liquid ethane (−180 °C) using a Leica GP plunger (Leica Microsystems, Austria).
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bioRxiv - Neuroscience 2022Quote: ... The brain was mounted on the vibratome stage using adhesive and placed in the slicing chamber submerged in partially frozen modified aCSF (Leica VT1000S, Leica Microsystems, Germany). 300um coronal sections were taken along the extent of the MGN (AP ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
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bioRxiv - Systems Biology 2023Quote: ... The frozen sample carriers were mounted on a cryo sample holder in the Leica EM VCM loading station (Leica Microsystems, Vienna, Austria) at liquid nitrogen temperature ...
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bioRxiv - Microbiology 2022Quote: ... Grids were blotted for 5 s and plunge-frozen in liquid ethane cooled by liquid nitrogen to approximately -195 °C using an EM GP Plunge Freezer (Leica, Wetzlar, Germany).
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bioRxiv - Cell Biology 2023Quote: ... Seventy nm-thick frozen-hydrated sections were cut at −150°C in a Leica UC7/FC7 cryo-ultramicrotome (Leica Microsystems, Vienna, Austria). The EM grid was positioned underneath the ribbon using a Leica micromanipulator (Studer et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Eight-day-old cotyledons either grown under LL or at 24 h after HL exposure were cut into small pieces and frozen in a high-pressure freezer (Leica EM Ice). To analyze the ultrastructure of chloroplasts ...
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bioRxiv - Cell Biology 2023Quote: ... were quick-frozen by high-pressure freezing using an HPM 010 high-pressure freezing machine according to the manufacturer’s instructions (Leica Microsystems, Wetzlar, Germany). Frozen specimens were transferred to the cold stage of a Balzers BAF 400 apparatus and fractured at -120° under a vacuum of ~ 1 × 10−6 mbar ...
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bioRxiv - Microbiology 2023Quote: ... Samples for milling were frozen on 2/2 200 gold mesh grids by plunge freezing in Leica EM GP2 or GP1 plunge freezer (Leica Microsystems, Germany) at 10°C and 90% relative humidity ...
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bioRxiv - Physiology 2023Quote: ... Cryosectioning of previously frozen placentas was performed with 10-mm-thick sections using a cryostat (Leica CM1860-UV, Leica Biosystems, Nussloch, Germany). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were embedded in OCT and frozen on dry ice and stored at -80°C until sectioning on a cryostat (Leica CM3050-S). Brain sections were mounted at 10 μm thickness on Superfrost Plus slides (1255015 ...
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bioRxiv - Neuroscience 2023Quote: ... Serial fresh frozen sections (18 µm thick) were cut through the entire hippocampus using a cryostat (Leica 1510, Leica Microsystems, Wetzlar, Germany) and stored at -80°C ...
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bioRxiv - Biophysics 2024Quote: ... 4 μL of 1 mg/mL of proteoliposomes were deposited during 30 s on the grid before flash frozen in liquid ethane with an EM-GP plunger (Leica Microsystems, Germany). Images were taken under low dose condition on a Glacios electron microscope (Thermofisher ...
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bioRxiv - Cancer Biology 2024Quote: ... The frozen samples were stored at -20°C in preparation for tissue sectioning using a cryostat (Leica CM3050 S, 10 μm thickness).
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Cell Biology 2020Quote: ... were transferred to 100 μm deep membrane carriers and high pressure frozen with a Leica EM PACT2 or Leica EM ICE freezers (Leica Microsystems, Wentzler, Germany). All samples were processed by freeze substitution in a Leica AFS2 temperature-controlling machine (Leica Microsystems ...
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bioRxiv - Cell Biology 2019Quote: ... EM grids impregnated with cells were sandwiched between a 20 μm–thick copper foil and a flat aluminum disc (Engineering Office M. Wohlwend, Sennwald, Switzerland) and frozen with an HPM 010 high-pressure freezer (Leica Microsystems, Wetzlar, Germany) as described (Tsuji et al. ...
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bioRxiv - Neuroscience 2020Quote: ... brains were frozen in Tissue–Plus (Fisher Healthcare, Houston, TX, USA) and sectioned coronally at 40 μm using a cryostat (CM3050S, Leica Microsystems, Nussloch, Germany). Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fresh-frozen brains were sectioned at 20-micron thickness in the coronal plane with a freezing cryostat (Leica SM2000R; Leica Microsystems, Wetzlar, Germany) and thaw-mounted on poly-L-lysine-coated slides ...
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bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ul of sample were pipetted onto the grids and the sample plunge frozen in liquid ethane using a Leica GP Plunge freezing device (Leica Microsystems, Heidelberg, Germany). The grids were then transferred under liquid nitrogen into a Gatan 626 Cryotransfer system (Gatan ...
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bioRxiv - Microbiology 2022Quote: ... and plunge frozen into a liquid ethane/propane mixture (pre-cooled with liquid nitrogen) using an EM GP2 automatic plunger (Leica Microsystems, Wetzlar, Germany)45 ...
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bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... and then frozen in isopentane cooled in liquid nitrogen before being sectioned coronally at 20 μm thickness with a cryostat (Leica Microsystems, Wetzlar, Germany). Sections were mounted on Superfrost plus slides (Thermofisher ...
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bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Biophysics 2022Quote: ... transferred to W5 buffer, and frozen on a glow-discharged grid (AG150P 200 mesh Cu, Zhongjingkeyi Technology) using a high-pressure freezer (Leica HPM100, Leica Microsystems). The grid was then transferred to Leica UC7+FC7 (Leica Microsystems ...
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bioRxiv - Cell Biology 2019Quote: ... were high-pressure frozen as described using an either an EM PACT2+RTS or an EM ICE high-pressure freezer (Leica Microsystems, Vienna, Austria)(Muller-Reichert et al. ...