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Citations for Leica :
701 - 750 of 1348 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Perfused brains (4% paraformaldehyde) were sectioned at 50-60 µm using a vibratome (Leica VT1000, Leica Microsystems, Nussloch GmbH, Germany). Sections were washed twice for 5 minutes each in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: Mice brains were harvested after perfusion with 4% PFA in PBS1X and post-fixed in 4% PFA for 2 hours on ice or at 4°C.Brains were then washed with 1XPBS three times and cut in cold PBS1X using a vibratome (Leica VT1200S), in 40 µm thick sections.Brain sections were mounted onto positively charged microscope slides (Superfrost PlusTM Microscope Slides White Tab ...
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bioRxiv - Cell Biology 2024Quote: Mice lung specimens were fixed in 4% paraformaldehyde for 48 h and subsequently embedded in paraffin using ASP200S and EG1150H (Leica). At the time of death ...
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bioRxiv - Cell Biology 2024Quote: ... kidneys were embedded in low-melting agarose (4%) and cut into 300 µm slices using a vibratome (VT1200 S, Leica). Slices were maintained in Krebs-Henseleit-Buffer carbonated with 95% O2/5% CO2.[24] For imaging ...
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bioRxiv - Cancer Biology 2020Quote: ... shRalA and shRalB cells with a Nanoject microinjector 2 (Drummond) under a M205 FA stereomicroscope (Leica), using micro-forged glass capillaries (25 to 30 mm inner diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were first permeabilized with heat in Bond Epitope Retrieval solution 2 (pH 9.0, Leica - AR9640) at 95°C for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100°C for 30mins ...
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bioRxiv - Microbiology 2021Quote: ... They were then freeze substituted in a EM AFS 2 system (Leica Microsystems GmbH, Wetzlar, Germany) and embedded into Epon resin ...
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bioRxiv - Neuroscience 2020Quote: ... (2) The SHG signal was collected from above the retina through a x40 0.8 NA (Leica) objective ...
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bioRxiv - Synthetic Biology 2022Quote: ... #1 prior to plunging into liquid ethane (−182°C) in a Leica EM GP 2 (Leica) vitrification robot (automated blot pressure ...
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bioRxiv - Plant Biology 2021Quote: ... The roots were washed with 1/2 MS media and imaged by fluorescence microscopy (Leica, M205FA). The quantitative measurement of ROS staining intensity was performed as average pixel intensities using ImageJ software [28].
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bioRxiv - Cancer Biology 2020Quote: ... At least 2× step sections per lung were stained on a Bond Automated Stainer (Leica Biosystems) using standard protocol F and Target Retrieval Solution pH 6.0 (Dako ...
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bioRxiv - Plant Biology 2020Quote: ... leaf discs were collected 2 dpi and observed using a Leica TCS SP8 (Leica, Mannheim, Germany) confocal microscope with the following excitation wavelengths ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were mounted in 2% agarose and sectioned at 50μm using a vibratome (Leica VT1000s, Germany). Coronal sections were used for all brains ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were mounted in 2% agarose and sectioned at 50 μm using a vibratome (Leica VT1000s). Coronal sections were used for all brains ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Neuroscience 2023Quote: ... embedded in 2% agarose and sectioned to 50-μm coronal/sagittal sections by a vibratome (Leica). For OE sections ...
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bioRxiv - Neuroscience 2022Quote: ... at - 45°C for 2 days and UV-polymerized for 1 day in EM AFS2 (Leica). The aBA-containing polymerized blocks were further sliced into 100 nm thickness by an ultra-microtome (Leica) ...
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bioRxiv - Cancer Biology 2024Quote: ... unstained sections were heated for 30 minutes in BondTM Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems Newcastle LTD ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections (2 μm thickness) obtained with a Leica RM2245 rotary micro-tome (Leica Biosystems) were mounted on glass slides (Superfrost Plus ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen was unmasked with Tris-EDTA pH 9 (Bond Epitop Retrival Solution 2 Leica, cat# AR9640). Both mouse monoclonal anti-Ki67 CloneMIB-1 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... at −45 °C for 2 days and UV polymerization for 1 day with EM AFS2 (Leica). UV-polymerized blocks containing the NAcSh were sliced by an ultramicrotome (Leica) ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Molecular Biology 2021Quote: ... The paraffin blocks transferred to room temperature and the blocks sections cut 4 mm thick in a rotatory microtome (Leica RM2135) and mounted in glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were removed and post-fixed in PBS containing 4% paraformaldehyde over night before cutting 50μm thick coronal sections on a vibratome (VT 1000 S from Leica, Wetzlar, Germany). Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Neuroscience 2020Quote: Brains were embedded in agar (3-4% w/v dissolved in dH2O) before being cut into 50 μm coronal sections on a vibrating microtome (VT1000S; Leica Microsystems). Free-floating tissue sections were collected in series ...
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bioRxiv - Cell Biology 2021Quote: ... whereas samples for STEM were attached to a sticky Post-It-Note (as described in (31)) and coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The STEM samples were then washed with Buffered Oxide Etchant (diluted 6:1 with surfactant ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
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bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted from the skull and stored in 4% formaldehyde before being cut in sagittal sections (50 μm) using a vibratome (Leica VT1000) and stained with cresyl violet ...
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bioRxiv - Bioengineering 2020Quote: ... and cells were cultured for 4 hours at 37 °C in the climate chamber of a Leica SP8 Confocal system (Leica Microsystems) microscope ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Plant Biology 2020Quote: ... by infiltration for 10 min in a vacuum and after further incubation for 4 h processed using the Leica Semi-Enclosed Benchtop Tissue Processor TP1020 (Leica Biosystems) for an ethanol series (75 % ...
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bioRxiv - Bioengineering 2021Quote: ... according to standard histological procedures and 4 μm thick sections of the paraffin blocks were cut on the microtome (Leica, HM325). The paraffin sections were stained with Haematoxylin-Eosin (HE ...
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bioRxiv - Neuroscience 2021Quote: ... brains of perfused mice (n=4 per group) were serially cut into 35 μm-thick coronal sections using cryostat (Leica, Germany). Sections were permeabilized in PBS containing 0.3% Triton X-100 for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Brains electroporated with the pCAG-mRFP;Hes5p-GFP plasmid were dissected 11 h after electroporation and embedded in 4% Agarose in complete HBSS medium (Polleux and Ghosh, 2002) and sectioned using a vibratome (Leica VT1200S). Electroporated areas of the lateral cortex were microdissected under the fluorescent dissecting scope (Zeiss Axio Zoom) ...
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bioRxiv - Immunology 2022Quote: ... grids were metalized by platinum sputtering (4 nm) using a Leica ACE600 cryo-sputter coater equipped with a cryo-stage (Leica Microsystems). Metallized samples were then transferred to a pre-cooled Crossbeam 550 FIB-SEM microscope (Carl Zeiss NTS GmbH ...