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Citations for Leica :
651 - 700 of 815 citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Embryonic left and right hippocampus was microdissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in Lepagnol-Bestel et al ...
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for blocking (1 hour, room temperature) and with a mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica) or a mouse anti-γ-SG 1:100 (NCL-L-g-SARC ...
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bioRxiv - Immunology 2020Quote: ... Non-invasive intravital imaging of mouse ear was performed using Leica DIVE (Deep In Vivo Explorer) inverted confocal microscope (Leica Microsystems) with full range of visible lasers (Spectra Physics) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using microhematocrit capillary tubes (10 μL) and measured the hematocrit rate for each mouse using a Critocaps Micro-Hematocrit Capillary Tube Reader (Leica Microsystems) (22).
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bioRxiv - Neuroscience 2022Quote: ... then the right OB from each mouse was embedded in 6 % agarose and sliced coronally at 50 μm using a vibratome (Leica VT1000S).
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bioRxiv - Biophysics 2023Quote: 5 µm sections of formalin-fixed and paraffin-embedded (FFPE) mouse tail specimens were H&E-stained by using an automated stainer (Leica XL) at University of Manchester’s Histology Core as previously described [11 ...
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Immunology 2021Quote: ... Confocal images were acquired 24 h after hindbrain injection with the different recombinant S1 proteins on a widefield microscope (Leica THUNDER imager) with a 10x/0.32 objective using a DFC 9000 GTC sCMOS camera (Leica ...
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bioRxiv - Neuroscience 2022Quote: ... Figures from double immunofluorescence labeling of GLP-1R and GFAP proteins were obtained on a Confocal Leica SP5 microscope (Leica Microsystems, France).
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bioRxiv - Cancer Biology 2020Quote: ... and BOND Polymer Refine Detection kit (DS9800, Leica Biosystems, Newcastle, UK) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Polymer detection system (Bond Polymer Refine Detection Kit #DS9800, Leica Biosystems) was used for horseradish peroxidase signal amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... using the BOND Polymer Refine Red Detection Kit (Leica Biosystems, DS9390). Whole slide scanning was performed on an Aperio AT2 digital whole slide scanner (Leica Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... The Bond Polymer Refine DAB Detection kit (Leica Biosystems, DS9800 DAB) was used and all procedures followed manufacture’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... Staining was visualized using the BOND Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems) as instructed by the manufacturer ...
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bioRxiv - Physiology 2023Quote: ... and staining using the Bond Polymer Refine Detection Kit (DS9800, Leica). Whole slides were then scanned using an Aperio AT2 slide scanner (Leica ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained with the Bond Polymer Refine Detection Kit (DS9800, Leica). The slides were scanned with a whole-slide scanner (Aperio AT2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and hematoxylin (Bond Polymer Refine Detection Kit, Leica Microsystems, Cat# DS9800) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and then incubated in blocking solution containing 10% normal donkey serum (017-000-121 ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin-embedded tissues was carried out on a Discovery XT automated slide stainer using a mouse anti-human monoclonal antibody for FOLR1 (Novocastra Clone BN3.2; Leica Biosystems, Wetzlar, Germany) at 15 µg/ml after antigen retrieval with Cell Conditioning 1 (CC1 ...
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bioRxiv - Neuroscience 2022Quote: ... each mouse brain was sectioned into 30-μm-thick coronal slices with a freezing microtome (CM30503, Leica Biosystems, Buffalo Grove, IL, USA). The sections were first washed in PBS three times for 5 min each and incubated in blocking solution for 2.5h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... The left and right hippocampus was micro dissected from genotyped P21 mouse brains using a laser-assisted capture microscope (Leica ASLMD instrument) with Leica polyethylene naphthalate membrane slides as described in ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) then placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany) and secured with ear bars into a nose cone delivering isoflurane to maintain anesthesia ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.8% oxygen in an induction chamber (VetEquip, Livermore, CA) and placed in an Angle Two mouse stereotaxic frame (Leica Biosystems, Wetzlar, Germany). Mice were given a subcutaneous injection of meloxicam (2 mg/kg ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Plant Biology 2019Quote: For BiFC assays Yellow Fluorescent Proteins (YFP) were visualized using a Leica TCS-SP2 laser scanning confocal microscope (LSM) (Leica Microsystems, Heidelberg, Germany) with a 63×1.4 NA oil-objective lens and processed using Leica confocal software v.2.61 about 16 h post-transformation ...
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Cell Biology 2019Quote: The gastrocnemius and quadriceps muscles were isolated from mouse hind limbs and dehydrated in a 30% sucrose solution and then sectioned in a constant cold section box (Leica CM 3000, Germany) at −20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Cell Biology 2023Quote: Fresh mouse kidneys were embedded in low-melting agarose and cut into 300 μm slices using a vibratome (LEICA VT1200 S, Mannheim, Germany). Slices were maintained in carbogenated Krebs-Henseleit-Buffer.14 AKS were incubated with 5 μM propidium iodide for 5 min to label cells damaged by cutting.
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were agroinfiltrated to transiently express the protein of interest and examined with a Leica TCS SP5 Laser Scanning Confocal Microscope (Leica Microsystems, Exton, PA, USA) using a 63x water immersion objective NA 1.2 (HCX PL APO) ...
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bioRxiv - Immunology 2022Quote: ... The antibody was detected using Bond Polymer Refine Detection kit (Leica Biosystems) with diaminobenzidine (DAB ...
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bioRxiv - Physiology 2020Quote: ... and staining using the BondTM Polymer Refine Detection kit (DS9800, Leica Biosystems). Brightfield images were acquired using a Leica DM 1000 LED microscope and processed using Leica Application Suite software (Leica Biosystems).
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bioRxiv - Cancer Biology 2020Quote: ... before peroxidase block was performed using an Intense R kit (Leica, DS9263). Caspase 3 was stained at a previously optimised dilution of 1/500 and Ki67 at 1/100 before washing with Leica wash buffer and then application of rabbit envision secondary (Agligent ...
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bioRxiv - Immunology 2022Quote: ... Slides were counterstained with hematoxylin (Bond Polymer Refine Detection Kit, DS9800, Leica Biosystems.
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bioRxiv - Cancer Biology 2022Quote: ... before peroxidase block was performed using an Intense R kit (DS9263, Leica) for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... with the Bond Polymer Refine Kit (cat. DS9800 Leica Biosystem, Richmond, IL). Immunostaining was performed using the Bond RX automated system (Leica Biosystems ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... the slides were stained with the Bond Refine Detection Kit (Leica Biosystems), according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... For the IHC portion a Bond Refine staining kit (Leica Biosystems, DS9800) was used with a standard protocol minus the peroxide blocking step which was deleted ...