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Citations for Leica :
501 - 550 of 815 citations for Mouse WD repeat containing protein WRAP73 WRAP73 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Microbiology 2022Quote: ... and the success of the intravitreal injection was confirmed by visualizing the fluorescein-containing vitreous through the dilated pupil with a Leica M620 TTS ophthalmic surgical microscope (Leica Microsystems, Inc). Eyes with significant hemorrhage ...
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bioRxiv - Microbiology 2022Quote: ... The eye was examined and the success of the subretinal injection was confirmed by visualizing the fluorescein-containing bleb through the dilated pupil with a Leica M620 TTS ophthalmic surgical microscope (Leica Microsystems, Inc). Eyes with significant hemorrhage or leakage of vector solution from the subretinal space into the vitreous were excluded from the study ...
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Molecular Biology 2023Quote: ... Acute brain slices containing perirhinal cortex and hippocampus were prepared at 275 μm thickness using a vibratome (Leica VT1200 S, Wetzlar, Germany). Slices were incubated for 25 min at 35° C and then transferred at room temperature into a custom made holding chamber.
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bioRxiv - Neuroscience 2023Quote: ... Sections from electrophysiological or calcium imaging experiments containing the vH were examined with a fluorescent microscope (M205 FCA, Leica Microsystems, Wetzlar, Germany) using a 2.0x magnification objective ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was cut into 400 µm thick horizontal slices containing the hippocampal formation with a vibratome (Leica VT 1200S, Leica Biosystems, Germany) and slices were transferred to interface-type recording chambers perfused with warm and carbogenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2023Quote: ... Formalin-Fixed Paraffin-Embedded blocks containing tumor or matched normal patient samples were cut into sections of 4μm thickness using a microtome (Leica, HistoCore RM2125 RTS) (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Neuroscience 2024Quote: ... Tilted at 30° horizontal cortico-striatal slices or horizontal slices containing SNpc were prepared on a vibratome (Leica VT 1200S, Leica Biosystems) in ice-cold choline chloride-based cutting solution of the following composition (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal slices containing DCN were cut at 210 µm in ice-cold sucrose solution on a vibratome ((VT1200S; Leica Microsystems, Wetzlar, Germany) or (7000smz-2 ...
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bioRxiv - Genetics 2021Quote: ... Protein localization was visualized by fluorescence confocal microscopy on a Leica SP5 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured with LASAF as described previously [20].
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was quantified as positive pixels using Aperio Positive Pixel Count Algorithm software (Leica Biosystem, IL).
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein localization was analyzed 24-48 hours after infiltration by confocal laser scanning microscope (TCS SP8, Leica). GFP was excited at 488 nm and the fluorescence detected between 498-525 nm.
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bioRxiv - Physiology 2023Quote: ... or BondTM proteolytic enzyme kit (AR9551, Leica). Sections were then incubated with the indicated antibodies in BondTM primary antibody diluent (AR9352 ...
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bioRxiv - Cancer Biology 2023Quote: ... or Bond proteolytic enzyme kit (AR9551, Leica). Thereafter ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed mouse brains and monkey or human brain samples were sectioned with a cryostat (model CM3050S, Leica) or a vibratome (Leica ...
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bioRxiv - Neuroscience 2019Quote: ... unfixed C57BL/6 mouse brains (N = 3) were embedded in tissue freezing medium (Leica Biosystems, Richmond, UK), frozen on a dry ice ethanol bath ...
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bioRxiv - Systems Biology 2022Quote: ... Two tile scans of 9 images each per mouse for quantification were acquired using confocal microscopy (Leica SP8 ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse skeletal muscle (quadriceps) was mounted on a piece of cork in Tissue Freezing Medium (12020108926, Leica) with fibers oriented perpendicular to the cork and then snap-frozen in liquid N2-cooled 2-methylbutane and stored at -80°C until sectioning ...
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bioRxiv - Pathology 2021Quote: ... using monoclonal mouse anti-DYS1 (Rod domain, no. NCL-DYS1; clone no. Dy4/6D3, Leica Biosystems, Wetzlar) as primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse coordinates: AP 1mm-1.4mm from bregma) were sliced on a vibrating microtome (Leica Biosystems, VT1000 S). Serial sections were stored in 0.1M PBS with 0.2% sodium azide until processing ...
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bioRxiv - Neuroscience 2022Quote: Images (n=5 per brain area per mouse) were acquired using a SP8 confocal microscope (LEICA, Germany) with a 63x oil immersion objective (PL APO CS2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse lung sections were imaged at 20X magnification using an Aperio Digital Pathology Slide Scanner (Leica Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Neuroscience 2021Quote: ... and 60 µm thick coronal slices containing the CA1 region of hippocampus were cut on an oscillating blade vibratome (Leica VT1000, Leica, Germany) and transferred to PBS ...
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bioRxiv - Cell Biology 2020Quote: ... the cell culture insert was removed and the blocks of resin containing the embedded cells were trimmed and finally cut using an ultra-microtome (Leica Microsystems, Wetzlar, Germany). Ultra-thin sections with an average thickness of 55nm were transferred on formvar-coated copper grids and stained with lead citrate ...
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bioRxiv - Immunology 2021Quote: ... Cells were extensively washed and mounted with VECTA SHIELD (containing DAPI) mounting medium and observed using a 63x oil objective on a confocal microscope (Leica SP8, Wetzlar, Germany).
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... and parasagittal slices containing the vermis of the cerebellum were cut at 350 mm on a Leica VT1200 (Leica Microsystems, Buffalo Grove, IL). Sections were incubated in a holding chamber maintained at 32°C ...
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bioRxiv - Neuroscience 2020Quote: Brain samples containing the hippocampus were cut serially into 16-μm-thick coronal sections using a cryostat (Leica CM 3050; Leica, Welzlar, Germany) and mounted onto glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... A block of the left hemisphere containing the entire frontal and parietal cortices was prepared and cut in the coronal plane with a vibratome (Leica model VT 1000S) into serial 150 μm-thick sections ...
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bioRxiv - Neuroscience 2020Quote: ... Horizontal slices (400 μm) containing the thalamus were cut in ice-cold protective recovery solution using a vibratome (VT1200, Leica Biosystems, Wetzlar, Germany). Slices were trimmed to remove the hippocampus and the hypothalamus ...
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bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL). For identification of DAT-specific deletion of PERK and consequential reduction of phosphorylated eIF2α (p-eIF2α ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The brains were quickly dissected and coronal midbrain slices containing the DRN (300 μm) were obtained using a vibratome (VT1200S; Leica Biosystems, Wetzlar, Germany). The brain slices recovered at 34 °C for 30 min in a chamber containing the choline-Cl-based aCSF described above ...
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was removed and coronal slices (200 μm) containing either the parabrachial nucleus (PBN) or the periaqueductal gray (PAG) were sectioned on a vibratome (Leica VT1200S, Wetzlar, Germany) in ice-cold oxygenated sucrose solution (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... Coronal brain sections (45-μm thickness) containing the amygdala and surrounding regions were cut using the cryostat microtome (CM1900, Leica Microsystems, Nussloch, Germany). Sections were treated with 3% H2O2 for 10 min and then blocked with 0.1% Triton X-100 in TBS containing 2% BSA and 2% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Neuroscience 2021Quote: ... Coronal slices containing the IL were cut at a thickness of 240 μm using a model VT1200S vibratome (Leica Microsystems, Buffalo Grove, IL). After resting 1 hr at 37°C in aCSF ...
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bioRxiv - Microbiology 2022Quote: ... The sample carriers containing the samples were mounted on a cryo sample holder in the Leica EM VCM loading station (Leica Microsystems, Vienna, Austria) at liquid nitrogen temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 400 μm thick horizontal slices containing the cortex and hippocampus were cut with a vibratome (OTS-4500, Electron Microscopy Sciences /Leica VT1000s, Leica Biosystem) under ice-cold ACSF ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was cut into 400 µm thick horizontal slices containing the hippocampal formation with a vibratome (Leica VT 1200S, Leica Biosystems, Germany) and slices were transferred to interface-type recording chambers perfused with warm and carbogenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...