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Citations for Leica :
601 - 650 of 702 citations for Simian Virus 40 Large Tumor Antigen Amino Terminal Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and post-fixed for at least 24 hours before sectioning 40 μm thick sections in the horizontal plane on a vibratome (Leica VT1000S). Sections to be stained with COX4 underwent antigen retrieval by boiling free floating sections in 1.7ml tubes for 3 min in nanopure water ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Cancer Biology 2021Quote: ... SA-β-gal positive cells were quantified based on three independent images from different stained regions analyzed by digital inverted light microscopy (40× phase-contrast; Leica DMi1). To evaluate senescent gene signature and SASP activity ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred into 30 % sucrose in phosphate buffer until they sank down before they were cut into 40 µm- or 60 µm- thick coronal sections using a dry-ice-cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at −20 °C in cryoprotectant solution (25 % ethyleneglycol ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were frozen and coronal sections of 40 µm containing the AON and the anterior piriform cortex (PCx) were cut on a sliding microtome (Leica, Germany) and collected as serial tissue sections spaced by 240 µm.
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bioRxiv - Neuroscience 2022Quote: ... The mouse brains were dehydrated in 30% sucrose and sectioned at a thickness of 40 μm with a cryostat (Leica Microsystem), and the brain sections were immunostained for mCherry (1:1000).
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Immunology 2022Quote: ... Larvae were then immediately mounted in 1% low-melting agarose containing 40 μg/ml tricaine and live imaged overnight using confocal microscopes (Zeiss 780 NLO or Leica SP8). The fluorescence intensity over time was calculated using SUM intensity projections ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were removed and placed in additional 10% formalin phosphate until sectioning into 40 µM thick sections via cryostat (CM 3050s; Leica Biosystems). Brain sections were stained with cresyl violet in order to visually confirm tetrode recording sites.
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bioRxiv - Neuroscience 2021Quote: Mouse brains were fixed in 4% paraformaldehyde and cut into 40-μm-thick sections using a vibratome (Leica Biosystems, Nussloch, Germany). The sections were blocked using blocking solution (X0909 ...
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bioRxiv - Neuroscience 2022Quote: The fixed brains were sliced in PBS into coronal sections (40 μm) at a Leica VT1000S vibratome (Leica Biosystems, Wetzlar, Germany). The coronal sections at the hippocampal level (Bregma −1.34 mm to −2.70 mm according to Franklin and Paxinos ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were dissected and post-fixed for at least 24 hours before sectioning 40-50 μm thick sections in the horizontal plane on a vibratome (Leica VT1000S). All brain sections immunostained with MCU or COX4 underwent antigen retrieval by boiling free floating sections for 3 min in 1ml of nanopure water ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were flash frozen with crushed dry ice and coronal sections (40 μm) containing the BNST were collected using a cryostat (Leica Microsystems) at −20° C ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were incubated in 30 % sucrose in phosphate buffer for two days and cut into 40 μm coronal sections using a dry-ice-cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4 °C in cryoprotectant solution (25 % ethyleneglycol ...
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bioRxiv - Neuroscience 2019Quote: ... The slices were then re-sectioned at 40 µm thickness using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL) and mounted on a microscope slide using Fluoromount-G mounting medium (Cat# 0100-01 ...
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bioRxiv - Neuroscience 2019Quote: ... embedded them in agar and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
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bioRxiv - Physiology 2020Quote: ... cells were washed once with DPBS and read by a fluorescence microplate reader (excitation 485/20 nm and emission 528/20 nm) or imaged using a LEICA DMI6000 B inverted microscope at 40× magnification (CTR6000 laser, Leica Microsystems). Fluorescence intensity was normalised to total protein content or nuclei number (using DAPI staining) ...
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bioRxiv - Neuroscience 2021Quote: ... brains were cut into sagittal (40 μm) sections using a Leica VT-1200 vibrating blade microtome (Leica Biosystems, Buffalo Grove, IL). Parasagittal sections were immersed in 2% osmium tetroxide (OsO4 ...
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bioRxiv - Cell Biology 2021Quote: Live-cell imaging was carried out on a Dragonfly spinning disk confocal microscope (Andor Technologies) equipped with Okolab boldline incubator (Okolab) and mounted on a Leica DMI8 using 40 X objective (Leica Camera). Focus stability was maintained using the Perfect Focus System (PFS ...
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bioRxiv - Biophysics 2021Quote: ... Cryo-planing of approximately 40 μm of the surface was performed in a cryo-microtome (EM UC6/FC6 cryo-microtome, Leica Microsystems) using a 45° diamond trimming knife with a clearance angle of 6°C (DiAtome) ...
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bioRxiv - Developmental Biology 2022Quote: Images were ftaken under 40 X or 63 X objectives on a laser-scanning confocal microscope (Zeiss LSM980; Leica microsystems; Germany). Images were analyzed using ImageJ (version 1.43 U ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence images from 9 fields of view were captured in each well using a 1.5 pin hole and a 40 x objective of a Leica SP8x confocal microscope (Leica Microsystems, Germany). External CFW stained spore fluorescence was excited with a 405nm LED and collected on a HyD detector (410-450 nm ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were cut in the rostro caudal direction (40 μm) using a sliding microtome Leica SM2010R (Leica Microsystems, Heidelberg, Germany). Primary antibodies mouse anti-Tyrosine Hydroxylase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were cut into 40-,tm thick sections using a vibrating blade microtome (Leica VT1000 S, Leica Biosystems, Nussloch, Germany). The sections were washed in 0.1 M PBS (3 × 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... Seminal roots were observed with a fluorescence microscope at 460/40 nm emission and 510/30 nm excitation (DMR HC, Leica, Germany), but due to their relatively large diameter ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Neuroscience 2023Quote: ... 40-μm coronal sections from the injection site and the VTA were cut with a cryostat (CM3050S, Leica Biosystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2022Quote: ... brains were cut into 40 μm coronal sections using a dry ice–cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4°C in cryoprotectant solution (25% ethylene glycol and 25% glycerol in 0.1 M phosphate buffer).
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bioRxiv - Neuroscience 2022Quote: Mouse brains were drop-fixed overnight in 4% PFA in 1X PBS and transferred to a 30% sucrose solution in 1X PBS before slicing (40 or 50 mm) on a sliding microtome (Leica Microsystems). The slices were permeabilized and blocked in 3% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in 30 % sucrose in phosphate buffer for two days and cut into 40 μm coronal sections using a dry-ice-cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4 °C in cryoprotectant solution (25 % ethyleneglycol ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in optimum cutting temperature (OCT; Tissue-Tek) and sectioned coronally at 40 μm using a sliding microtome (Leica, HM450). For immunohistochemistry ...
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then transferred to PBS and 40 μm coronal consecutive brain sections were obtained employing a vibratome (Leica VT1200S, Leica Microsystems), collected in PBS and stored in cryoprotective solution (40% PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Embedded frozen brains were serially sectioned in the coronal plane at 40-μm thickness using a freezing microtome (CM3050S, Leica Microsystems). Every sixth section was sequentially transferred to 6-well plates of PBS for subsequent immunohistochemical staining.
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections (40 μm) through the rostro-caudal extent of the hippocampus were generated using a vibratome (Leica VT1000 S, Germany), and processed for immunohistochemistry ...
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bioRxiv - Bioengineering 2022Quote: ... PGP 9.5-immunoreactive nerve fibers present in the epidermis of each section were counted at a magnification of 40× under a light microscope (Leica ICC50D, Leica, Germany).
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Neuroscience 2023Quote: ... Brains were dissected and post-fixed for at least 24 hours before sectioning 40 μm thick sections in the horizontal plane on a vibratome (Leica VT1000S). All brain sections immunostained with MCU were washed in 1X PBS-0.1% Triton X-100 (0.1% PBST ...
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bioRxiv - Neuroscience 2023Quote: ... Then they were blocked in OCT and cryosectioned at 40 µm thick in the horizontal plane using Leica Cryostat (Leica CM1860). CTL (n=8 ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2023Quote: ... Half brains were fixed in 4% paraformaldehyde for 24h and sectioned at 40 μm thickness in the coronal plane on a vibratome (Leica VT1000S).
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bioRxiv - Microbiology 2023Quote: ... Slides were imaged using Leica TCS SP8-X microscope with HC Plan Apochromat 40× and 63× oil objective and analysed using Leica LAS X Software (Leica Microsystems).
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bioRxiv - Immunology 2023Quote: ... Stained thick sections were microscopically analyzed using a Leica SP8 confocal microscope equipped with an HC PL APO CS2 40× (NA, 1.30) oil objective (Leica Microsystem, Inc.) and images were processed with Leica LAS AF software (Leica Microsystem ...
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bioRxiv - Immunology 2023Quote: ... Mounted lung sections on the chamber slide were microscopically analyzed using a Leica SP8 confocal microscope equipped with an HC PL APO CS2 40× (NA, 1.30) oil objective (Leica Microsystem, Inc.) and images were processed with Leica LAS AF software (Leica Microsystem ...
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 40 µm in the coronal plane with a Leica SM 2000R freezing microtome (Leica Microsystems). The sections were stored at -20 °C in 24-well tissue culture plates filled with antifreeze solution (30% ethylene glycol ...
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bioRxiv - Neuroscience 2024Quote: ... brains were cut into 40 µm coronal sections using a dry ice–cooled copper block on a sliding microtome (Leica, SM2000R). Sections were stored at 4°C in cryoprotectant solution (25% ethylene glycol and 25% glycerol in 0.1 M phosphate buffer) ...
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bioRxiv - Neuroscience 2023Quote: Brains were cryoprotected with 30 % sucrose in 30 % sucrose 0.1 M PB (81 mM Na2HPO4, 19 mM NaH2PO4) and sectioned in the coronal plane (40 μm thick) using a Cryostat (Leica 3050S). For anti-HA immunostaining ...
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bioRxiv - Cancer Biology 2021Quote: ... Metaphase chromosomes were GTG-Banded by a conventional trypsin-Giemsa technique and karyotypes (20-40 metaphases per cell line) were analyzed using a capture and analysis Cytogenetic software (Citovysion Version 7.7, Leica Biosystems Richmont, inc.).
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bioRxiv - Neuroscience 2019Quote: ... frozen using dry ice and subsequently sectioned in the coronal plane (40 µm) using a cryostat (Leica Biosystems Inc, Buffalo Grove, IL). For Experiment 1 ...
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bioRxiv - Genetics 2019Quote: ... and examined with a Leica SP5DM6000B confocal microscope (488 nm argon and 561 nm DPSS laser, 40× oil immersion objective, NA = 1.3) (Leica Microsystems, Wetzlar, Germany).