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Citations for Leica :
551 - 600 of 702 citations for Simian Virus 40 Large Tumor Antigen Amino Terminal Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... equipped with a GFP filter (470 ± 40 nm excitation and 525 ± 50 nm emission) and documented with the attached camera (DFC425 C, Leica). Leaf discs were cut from positive fluorescent plants using a 7 mm cork borer and placed with the abaxial side up in the bottom of transparent Nunc® 96-wells plates (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Molecular Biology 2021Quote: ... The brains were sectioned sagittally into four series of 40-μm sections and two series of 20-μm sections with a cryostat (Jung CM3000 Leica). The 40-μm sections were mounted on glass slides for subsequent tissue dissection for total RNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S ...
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bioRxiv - Neuroscience 2019Quote: ... and serial sections of 40-nm thickness were obtained (within a few microns from the surface of tissues) using an ultramicrotome (UC7, Leica). The serial sections were picked on the grid coated with pioloform and counterstained with 1% uranyl acetate (7 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sagittal sections (40 μm) of the entire MEC were cut using a vibratome (VT 1200S, Leica, Wetzlar, Germany). Every fourth ection was collected for IHC ...
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bioRxiv - Cell Biology 2020Quote: ... using a Leica SP5DM6000B confocal microscope (488 nm argon and 561 nm DPSS laser, 40× oil immersion objective, NA = 1.3) (Leica Microsystems).
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bioRxiv - Plant Biology 2022Quote: ... The samples were analyzed using a Leica True Confocal Scanner SP8 microscope equipped with an HC PL APO CS2 40 × 1.10 water immersion objective (Leica, Germany) as described in Dahncke and Witte (2013) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Neuroscience 2022Quote: ... the perfused and postfixed brains were equilibrated in RNase-free 30% sucrose and then cut into 40-μm-thick coronal sections on a cryotome (Leica CM300, Leica). Floating brain sections were placed in chamber slides (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... solani IPO 2222 Tn5 mutants was analyzed using a Leica MZ10 F stereomicroscope with 10× and 40× magnifications combined with a Leica DFC450C camera (Leica) as previously described (Lisicka et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Images were taken every 0.6 μm of focal plane using z-stack function using oil immersion ×40/×60 objective (Leica Germany) at Nikon Eclipse Ti microscope (Nikon Instruments ...
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bioRxiv - Neuroscience 2020Quote: ... Imaging for Golgi-Cox staining was performed in bright field mode while Methoxy-X04 fluorescence was captured with a filter cube (excitation filter: BP 360/40 nm, dichroic mirror: 400 nm, emission filter: LP 425; Leica). The distance of the analyzed dendritic segments from the Aβ plaques was determined as the average distance of both segment end-points and the center of the segment to the respective plaque border in merged pictures using ImageJ software ...
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bioRxiv - Biophysics 2020Quote: All commercial microscope experiments were performed on a Leica DMi8 equipped with a Plan Apochromatic 40×/1.30 oil immersion objective (Leica #11506358), motorized XY and focus stages ...
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bioRxiv - Neuroscience 2021Quote: ... we set up a transmission microscope composed of a high NA collimator objective (LEICA HC PL APO 40×/1.1 W) on a 5-axis kinematic mount ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was thoroughly washed in 0.1 M PBS and thereafter sectioned (40 µm thickness) using a vibratome (Leica VT1000, Leica Microsystems). The sections were stored in 1x PBS at 4°C.
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bioRxiv - Neuroscience 2021Quote: ... microglial density quantifications and Iba-1:P2RY12 or Iba-1:TMEM119 colocalization analysis 40 µm free-floating sections were cut with a sliding microtome (Leica) and stained with relevant antibodies in 10% normal goat serum (NGS ...
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bioRxiv - Cell Biology 2022Quote: All images of optoDroplet plasmid-transfected HeLa cells were captured using a 40× objective and TCS SP5 confocal microscope (Leica) to observe IDR behaviour upon blue light activation (47) ...
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bioRxiv - Microbiology 2019Quote: ... The viability of wt-PCN and ov-PCN yeasts was determined by fluorescein diacetate-ethidium bromide staining [53] and detected by fluorescence microscopy (excitation bands: blue-BP480/40; green-BP515-560) (DMI6000B, Leica). Only suspensions containing at least 90% of viable yeasts were included in the studies ...
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bioRxiv - Neuroscience 2021Quote: ... the labelled specimens were included in a bloc of gelatin/albumin as described (Sedó-Cabezón et al., 2015) and sectioned at 40 μm in a vibrating blade microtome (Leica VT1000S). The whole epithelia or the epithelial sections were finally mounted in Mowiol medium.
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bioRxiv - Cell Biology 2021Quote: ... and stained with 0.1% toluidine blue to quantify the different germ cell types at 40× and 100× objectives using a high-resolution light microscope (Leica DM6000 BD ...
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bioRxiv - Neuroscience 2021Quote: ... brains were frozen in isopentane (−40°C) and sliced in serial coronal 40μm sections by using a cryostat (CM-1900, Leica, Germany). Samples were maintained in antifreeze solution before immunostaining procedure.
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Microbiology 2022Quote: ... Alexa 555 (Ex: 550, Dc: 570, Em: 576) and GFP (Ex: 470/40 nm Chroma ET470/40x, BS: LP 498 Leica 11536022 ...
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bioRxiv - Neuroscience 2023Quote: ... and brains were postfixed in 4% paraformaldehyde and preserved with 30% sucrose before sectioning at 40-µm on a freezing-sliding microtome (SM2010R, Leica). Sections were incubated overnight with indicated primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 4% agarose before being cut into 40 um coronal slices with a vibratome (Leica VT S10000) and stored in 0.1% PBS Sodium Azide ...
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bioRxiv - Neuroscience 2022Quote: Images of mounted bladders were acquired with an upright Thunder 3D Tissue Imaging Microscope DM6B-Z with motorized stage and 40 × 0.95 NA dry objective (Leica Microsystems) along with LAS X software (Leica Microsystems) ...
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bioRxiv - Biophysics 2022Quote: ... and proliferation were determined from optical images collected with 10x or 40× air lens and phase contrast using a Hamamatsu camera on a Leica DMIRE2 inverted microscope (Leica). For each condition ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal brain slices were taken at 40 µm using a cryostat at −20°C (Leica Biosystems Inc, Buffalo Grove, IL). The whole brain was collected ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μm slices were made of the entire dorsal hippocampus (dHPC) at -20 °C using a cryostat (CM1510 Leica Biosystems). Sections were stored individually in 96-well plates containing antifreeze solution (30% ethylene glycol ...
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bioRxiv - Neuroscience 2023Quote: ... in 0.1M phosphate buffer (PB) for 30-40 min and PFC containing sections of 50-100 μm thickness were prepared using a Vibratome (Leica) and stored in 0.1M PB with 0.05% Na-azide until further processing.
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bioRxiv - Molecular Biology 2023Quote: ... Frozen eyecups were sectioned to 40 μM thickness at -20°C on a Leica CM3050 S Cryostat (Leica, Wetzlar, Germany) onto TruBond 380 adhesive slides (Electron Microscopy Sciences ...
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bioRxiv - Genetics 2023Quote: ... a 488 nm excitation laser module and a GFP filter (Ex: 470/40 nm Chroma ET470/40x, BS: LP 498 Leica 11536022 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then sliced in coronal slices at 40 µm thick using a freezing microtome (Leica, Wetzlar, Germany; SM 2000R) and stored in 0.1 M PB.
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bioRxiv - Biochemistry 2022Quote: ... samples were capped in DAKO Mounting Medium and analyzed by a fluorescence microscope (Axiophot, Zeiss) at 40 × magnification or a confocal laser scanning microscope (SP5, Leica).
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bioRxiv - Neuroscience 2023Quote: ... Brains were post-fixed 24h in 4% PFA before being cut coronally and serially (10 series of 40 µm sections or 3 series of 100 µm sections for morphological studies) with a vibratome (Leica).
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bioRxiv - Neuroscience 2023Quote: ... 40 µm thick coronal sections were prepared using a vibratome (Microm HM650V, Thermo Scientific, Waltham, MA, USA, or Leica VT1000S) and stored at −20°C in a cryoprotective solution containing 20% glucose (Sigma ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were frozen at -20℃ and coronal sections (40 µm) cut in the frontal plane on a cryostat microtome (YD-1900, Leica). Coronal slices were stored in -20℃ in PBSAF (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Single photons were collected through a 40×/1.3 NA oil-immersion objective and detected on Hybrid Detectors (HyD) (Leica Microsystems) with a 570 – 610 nm ...
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bioRxiv - Biophysics 2023Quote: ... and immediately imaged on Leica Stellaris Will inverted stage scanning confocal microscope using a 40×/1.3 HC PL Apo CS oil objective (Leica Microsystems) at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Serial frozen sections of cervical (C8-T4), thoracic (T3-T12) and lumbar (T9-L3) segments (40 μm, transverse) were prepared using a cryostat microtome (Leica) and stored in antifreeze solution (Glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated with a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems, 3-7 min). The neurons were considered completely photoinactivated when the membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2024Quote: ... dehydrated brain was cut into 40-μm-thick coronal slices using a cryostat (Leica CM3050 S; Leica Biosystems, Wetzlar, Germany) and washed with 0.1 M PBS for 10 minutes ...
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bioRxiv - Physiology 2024Quote: ... Coronal brain sections of the arcuate nucleus were cut at a thickness of 40 μm on a cryostat (Leica, CM3050S). Sections were mounted onto SuperFrost Plus slides and stored at −80 °C with desiccant until the day of the immunohistochemistry procedure ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed in the same solution and 40 μm-thick coronal slices were cut at the vibratome (Leica). Two PMv coronal sections per animal were selected to perform c-fos immunostaining as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... the transgenic worms that express daf-42p::signal peptide::mCherry and grd-10p::gfp transgenes were observed under a fluorescence stereo microscope (Leica M205 FA). At 48 HAE ...
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bioRxiv - Molecular Biology 2020Quote: ... Brains were embedded in optimum cutting temperature (Tissue-Tek) and coronally sectioned at 40 μm using a sliding microtome (Leica, HM450). For immunohistochemistry ...
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bioRxiv - Neuroscience 2019Quote: ... brains were flash frozen using crushed dry ice and coronal sections (40 μm) containing the BNST were collected using a cryostat set to −20° C (Leica Microsystems). Sections were wet-mounted onto gelatin-subbed glass microscope slides ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...