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Citations for Leica :
601 - 650 of 694 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the epithelium at ALI was visualized for Green Fluorescent Protein (GFP) at 20X by fluorescent microscopy using 3i Marianas/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA), assessed for functional phenotypes (as described below) ...
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bioRxiv - Microbiology 2023Quote: ... Concentrations of stock solutions of NP and MP beads were calculated using a hemocytometer and a Leica DM IL microscope with a green fluorescent protein (GFP) filter (Leica microsystems, Wetzlar, Germany) with an attached CoolLED pE-300 ultraviolet light system (Andover ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... harvested from the Inbred immunocompetent & NOD.SCID treatment study mice were stained for Cm major outer membrane protein (MOMP) using an automated staining platform (Leica Bond RX, Leica Biosystems). After deparaffinization and heat-induced epitope retrieval in citrate buffer at pH 6.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody staining was completed using the Bond Polymer Refine IHC protocol and reagents (Leica, Wetzlar, Germany). Slides were counterstained on the Leica Autostainer XL (Leica ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody staining was completed using the Bond Polymer Refine IHC protocol and reagents (Leica, Wetzlar, Germany). Slides were counterstained on the Leica Autostainer XL (Leica ...
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bioRxiv - Genetics 2021Quote: ... Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used ...
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used: mouse anti-CD45 (Leica Biosystems, NCL-L-LCA, 1/70), mouse anti-CD3 (Leica Biosystems CD3-565-L-CE ...
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bioRxiv - Cancer Biology 2020Quote: ... The TMA slides were sequentially stained with antibodies specific for CD4 (T cells, 4B12, Leica Biosystems), CD8 (T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were applied for 60 mins and detected with Polymer Refine Detection Kit (Leica, DS9800). For detection of P63 ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical analyses were performed utilizing ready-to-use Ki67-specific antibody solution (K2, Leica Biosystems, PA0230). Sections were dewaxed ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies were diluted as follows: anti-p53 (CM5) (1:500, Leica Biosystems, NCL-L-p53-CM5p), anti-p21 (F-5 ...
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bioRxiv - Immunology 2024Quote: ... Ready to Use Antibody reagent was added as marker and incubated for 15 min (CD4: Leica Bio systems ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were agroinfiltrated to transiently express the protein of interest and examined with a Leica TCS SP5 Laser Scanning Confocal Microscope (Leica Microsystems, Exton, PA, USA) using a 63x water immersion objective NA 1.2 (HCX PL APO) ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
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bioRxiv - Immunology 2021Quote: ... Western blot was performed according to the supplier’s instructions using rabbit polyclonal anti-p53 antibody (CM5, Leica) and rabbit-monoclonal anti-vinculin antibody (EPR8185 ...
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bioRxiv - Microbiology 2021Quote: ... For both antibodies antigen unmasking was undertaken using Heat Induced Epitope Retrieval (HEIR) solution 1 (Leica AR9961) for 30 minutes at 100°C.
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bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...
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bioRxiv - Cancer Biology 2021Quote: ... five-micron sections of formalin-fixed paraffin-embedded tissue were stained using antibody against Androgen Receptor [(Leica AR-318-L-CE ...
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bioRxiv - Bioengineering 2022Quote: ... secondary and myofiber membranes were identified with anti-α-sarcoglycan antibody (Leica Biosystems, Wetzlar, Germany, 1:200) with an Alexa Fluor™ 568 secondary (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with the corresponding secondary antibodies and visualization systems (OptiView DAB for ALK and Leica Bond Polymer Refining Kit for ROS1 and BRAF ...
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bioRxiv - Genetics 2021Quote: ... were ruled out by a monoclonal antibody against α-synuclein (NCL-L-ASYN; Leica Biosystems, Wetzlar, Germany). The staging of AD was performed by using the ABC score according to the updated National Institute on Aging-Alzheimer’s Association guidelines (50) ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was then incubated in primary antibody solution made with 1:250 mouse anti-Ki67 (Leica Biosystems), 1% NDS ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of tissue-bound primary antibody was performed using the Bond Intense R Detection System (Leica Biosystems). The stained slides were converted to digital data using the NanoZoomer Digital Pathology 2.0-HT digital slide scanner (Hamamatsu).
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bioRxiv - Developmental Biology 2020Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using p53 polyclonal antibodies (NCL-L-p53-CM5p; Leica Biosystems). ChIPs were performed essentially as described previously (Kenzelmann Broz et al. ...
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bioRxiv - Neuroscience 2022Quote: ... slides of frontal cortex specimens stained with Clusterin antibodies were scanned on an Aperio AT2 scanner (Leica biosystems) at 20x magnification and digital slides analyzed using the QuPath platform (version 0.3.1 ...
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bioRxiv - Genetics 2020Quote: Reporter expression and fluorescent antibody-staining were imaged on an MSV269 stereoscope with a DFC365 FX camera (Leica). Freshly hatched adults (<30 minutes ...
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bioRxiv - Pathology 2020Quote: ... The primary antibodies were detected using the Bond Polymer Refine (DAB) Detection Kit (Leica Biosystems; Buffalo Grove, IL). Positive control mouse tissues for all antibodies were run in addition to negative controls performed using rat or rabbit isotype IgG.
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bioRxiv - Cancer Biology 2021Quote: The following primary antibodies were used: mouse anti-Synaptophysin (1:50, clone 27G12, Leica, NCL-L-SYNAP-299), rabbit anti-Chromogranin A (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubations were followed by signal detection using BOND Polymer Refine Detection Kit (Leica Biosystems, IL, USA) with Mayer’s hematoxylin nuclear counter stain as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with secondary antibodies and visualization were performed according to the manufacturer’s protocol (Novocastra Peroxidase Detection System, Leica). After each step ...
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bioRxiv - Immunology 2023Quote: ... Detection of the second primary antibody was performed using the Bond Polymer Red Refine Kit (DS9390, Leica Biosystems). Slides were counterstained ...
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bioRxiv - Neuroscience 2024Quote: ... or secondary antibodies (Table 1) were acquired using the Fast Lifetime Contrast (FALCON) enabled tau-STED module (Leica) with the time-gate on HyD-X detectors adjusted between 0.1 and 6 nanoseconds.
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bioRxiv - Developmental Biology 2020Quote: ... P53 stabilization upon doxorubicin and Nutlin-3a treatment was confirmed by Western blotting using p53 CM5 antibody (Leica Biosystems).
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bioRxiv - Immunology 2021Quote: ... Triple immunostainings were performed by incubating the same sections with CD8 antibody (clone 4B11, 1:50 pH9, Leica Novocastra) and anti-mouse Alexa Fluor 488-coniugated secondary antibody (1:500 ...
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bioRxiv - Pathology 2019Quote: ... A 1:3000 dilution of rabbit anti-CD163 antibody for 15 min and then anti-rabbit Polymer AP (Leica) for 20 min resulting in magenta-red labeling ...
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bioRxiv - Cancer Biology 2021Quote: ... A polymer detection system that includes an anti-mouse secondary antibody (DS9800, Novocastra Bond Polymer Refine Detection, Leica Biosystems) was then applied in the tumor samples ...
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bioRxiv - Cancer Biology 2023Quote: ... automated methods on the Leica Bond Max autostainer using a mouse monoclonal anti-human CD3 antibody (Leica, clone LN10). Whole slide images of IHC stained slides were digitally captured using an Olympus IX83 microscope at 10x magnification and fixed exposure times ...
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bioRxiv - Bioengineering 2024Quote: ... Dystrophin detection (both full-length and µDystrophin) has been performed by the antibody DysB (NCL-DYSB, Leica, 1:20) and its expression has been quantified by total protein normalization.
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bioRxiv - Cancer Biology 2024Quote: ... four-micron formalin fixed paraffin embedded (FFPE) tissue sections were incubated with the primary anti-ER 6F11 antibody (Leica) for 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested to prepare chromatin for immunoprecipitation using either p53 polyclonal antibodies (Leica Biosystems, Cat # NCL-L-p53-CM5p) or Mettl3 polyclonal antibodies (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were washed and the brains were mounted in Slowfade and imaged at a confocal microscope (Leica SP8) using a 63x glycerol objective ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μm sections from the TMA-2 human adenocarcinoma cohort were duplex stained using a Ventana autostainer with antibodies for cytokeratin AE1/AE3 (1:250, #NCL-L-AE1/AE3, Leica detected with Roche discovery yellow chromogen ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibody (polymer) incubation (10 minutes) and diaminobenzidine-based signal generation (10 minutes) were performed per manufacturer’s instructions (Leica # DS9800). Pictures were taken using SPOT Insight Gigabit camera and SPOT Imaging Software (5.1).
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated for 60 minutes and detected using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag-specific antibody was used at 1:100 dilution (Cat # R960-25, Invitrogen) (. Cells were imaged using a Leica DMi8 confocal microscope (63x ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed and stained with appropriate secondary antibodies and imaged using an inverted laser scanning confocal microscope (TCS SP5 X, Leica) using Leica Application Software (LAS ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...