Labshake search
Citations for Leica :
501 - 550 of 651 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
-
bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
-
bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
-
bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X). WT ...
-
bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X).
-
bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
-
bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
-
bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
-
bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: NCAM (Leica Biosystems, NCL-L-CD56-504, 1:50) and MYCN (Novus Biologicals ...
-
bioRxiv - Developmental Biology 2020Quote: ... Anti-filaggrin primary antibody staining (mouse monoclonal 15C10 from Leica Biosystems, Milton Keynes, UK) was followed by secondary antibody labelling (anti-mouse Alexa 488 ...
-
bioRxiv - Neuroscience 2021Quote: ... The Cy3™ - conjugated secondary antibody was detected by a DPSS laser (Leica, Germany) at 561 nm.
-
bioRxiv - Cancer Biology 2020Quote: ... Biomarker conditions were as follows: Ki67 (clone MM1 DO-7 mouse monoclonal antibody, Leica) was used at a 1:200 dilution with epitope retrieval solution 2 pre-treatment for 30 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tissue microarray slides were processed as above and incubated with EZH2 antibody (Leica Biosystems) for 1hr by Dr Frances Daley (Pathology Core Facility ...
-
bioRxiv - Cancer Biology 2023Quote: ... as well as mouse anti-CD4 monoclonal antibody 4B12 (1:1000, Leica Biosystems; AB_563560) and rabbit anti-IL-17 polyclonal antibody (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... The primary antibodies were detected using the BOND Polymer Refine Detection System (Leica, DS9800). As positive controls of the IHC ...
-
bioRxiv - Immunology 2023Quote: ... Ready-to use secondary antibodies Novolink Post Primary and Novolink Polymer (RE7260-CE, Leica) were used followed by either TSA Cy5 (SAT705A001EA ...
-
bioRxiv - Cell Biology 2024Quote: ... they were coupled with 1 mg/mL of anti-SAG1 antibodies (NCL-Tg, Leica).
-
bioRxiv - Plant Biology 2021Quote: ... the expression of TiAP1 and TaSYP51 proteins were observed and analysed using a confocal laser-scanning microscope (Leica SP5 - X, Germany) according to the methodology of Smigielski et al ...
-
bioRxiv - Molecular Biology 2020Quote: Cells expressing EGFP protein were visualized 48 h post-transfection by fluorescent microscopy using Leica DMi8 inverted microscope (Leica Microsystems, Germany) at magnification 10x ...
-
bioRxiv - Cell Biology 2022Quote: ... Each protein mixture (14 μM for each component) was injected into a homemade chamber and imaged using a Leica microscope (Leica DMi8) using a 40X objective lens ...
-
bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software). Images were acquired as z-stacks with a z-step size of 120 nm and a X-Y resolution of 37.9 nm ...
-
bioRxiv - Plant Biology 2022Quote: ... the protein fluorescence was confirmed with the Leica TCS LSI macroscope with Plan APO 5x and 20x objectives (Leica Microsystems, Germany), using the settings described previously83 ...
-
bioRxiv - Cell Biology 2023Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... The presence of Cas9 in the shoot apical meristem was investigated by observing the fluorescence of the Cas9-GFP protein (Venus-Cas9) using a confocal laser scanning microscope (TCS SP5, Leica Microsystems). The area of each cell within the SAM was calculated using SEM images of the SAM with Image J software (https://imagej.nih.gov/ij/download.html).
-
bioRxiv - Cell Biology 2020Quote: ... neural crest populations were captured at 4 days post-fertilation (dpf) using a Leica Sp8 and analyzed with the Leica Application Suite X (Leica Microsystems, Buffalo Grove, IL) software for image capturing ...
-
bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
-
bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...
-
bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
-
bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
-
bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and incubated with an anti-p53 antibody (1:5000; Leica Biosystems; Buffalo Grove, IL, USA) or an anti-GAPDH antibody (1:104 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibody signals were detected on a Leica DM6000 microscope equipped with a DFC390 camera (Leica). Data acquisition was performed with LAS AF (Leica) ...
-
bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
-
bioRxiv - Cancer Biology 2020Quote: ... then the samples were incubated in polymeric horseradish peroxidase (HRP) conjugated secondary antibody (Leica PV6121) for one hour followed by 5 five-minute 1xTBST washes ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antibodies were diluted as follows: p53 (CM5) (1:500, NCL-L-p53-CM5p, Leica Biosystems), p21 (F-5 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL ...
-
bioRxiv - Pathology 2021Quote: ... 200 uL of anti-mouse secondary antibody (Leica Cat# PV6114, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
-
bioRxiv - Pathology 2021Quote: ... 200 uL of anti-rabbit secondary antibody (Leica Cat# PV6119, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary mouse monoclonal antibodies were directed against synaptophysin (1:100, Leica Biosystems, Wetzlar, Germany, #PA0299), neurofilament ...
-
bioRxiv - Cancer Biology 2022Quote: ... slides were incubated with the corresponding secondary antibodies and visualization systems (Novolink Polymer Linker, Leica) conjugated with horseradish peroxidase ...
-
bioRxiv - Bioengineering 2023Quote: ... Micro-dystrophin detection has been performed by the antibody DysB (NCL-DYSB, Leica, 1:20) and its expression has been quantified by total protein normalization.