Labshake search
Citations for Leica :
501 - 550 of 633 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: Frozen coronal ovine hypothalamic blocks (n=4 per group) for in-situ hybridization were cut into 16µm sections using a cryostat (CM3050s Leica Microsystems, Ltd., Milton Keynes, UK), and thaw mounted onto poly-l-lysine coated slides (VWR International ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Neuroscience 2021Quote: ... and sacrificed via transcardiac perfusion with 10% sucrose (isotonic at 9.25% in distilled water; prewash) and 4% paraformaldehyde (PFA; fixative) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
-
bioRxiv - Neuroscience 2022Quote: ... embedded them in 4% w/v agar/PBS and prepared 40 µm thick slices using a vibrating blade microtome (VT1200S, Leica Microsystems, Buffalo Grove, IL). The slices were post-fixed in 4% PFA/PBS for 30 min at room temperature (RT ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.4 mM L-ascorbic acid and the pH was adjusted to 7.4) and then sliced on a vibratome (Leica, VT1200 S, Deerfield, IL, USA). Brain slices (300 μm thick ...
-
bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
-
bioRxiv - Plant Biology 2024Quote: ... 4 DAT seedlings were treated with 1 M PEP1 for 24h before they were imaged using confocal miscrscopes (Leica TCS SP8 and Zeiss LSM700). To visualize the GFP protein ...
-
bioRxiv - Cancer Biology 2024Quote: Cells were seeded on glass bottom dishes then image acquisitions were performed every 2 min for 4 using LiveSR spinning-disk microscope (Leica, Leica Microsystems GmbH, Wetzlar, Germany) with environmental control at 37°C and CO2 provider ...
-
bioRxiv - Neuroscience 2024Quote: ... following imaging and either underwent cardiac perfusion with phosphate buffered saline and 4% paraformaldehyde or had their brains extracted and post-fixed in 4% paraformaldehyde before being cut on a vibratome in 40 µm sections (Leica VT1000S, Deer Park, IL). Sections through the olfactory bulb were mounted on slides and were coverslipped using Fluoromount-G containing DAPI (SouthernBiotech ...
-
bioRxiv - Neuroscience 2024Quote: ... Sagittal 300-μm-thick slices were cut from the dorsal level of the hippocampus48 at 2-4 mm from midline using a vibratome (Leica VT-1200, Nussloch, Germany). Slices were stored in a reserve chamber containing slicing solution at 34°C for at least 30 min and subsequently at room temperature (20 – 25°C).
-
bioRxiv - Neuroscience 2024Quote: ... They were then perfused transcardially with ice-cold (4°C) phosphate buffered saline (PBS) using the Perfusion Two™ system (Leica Biosystems, Chicago, IL).
-
bioRxiv - Developmental Biology 2020Quote: ... and incubated with an anti-p53 antibody (1:5000; Leica Biosystems; Buffalo Grove, IL, USA) or an anti-GAPDH antibody (1:104 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibody signals were detected on a Leica DM6000 microscope equipped with a DFC390 camera (Leica). Data acquisition was performed with LAS AF (Leica) ...
-
bioRxiv - Microbiology 2020Quote: The following antibodies were used-anti-VP1 (NCL-ENTERO, clone 5-D8/1, Leica Biosystems) and cleaved caspase 3 (Asp175 ...
-
bioRxiv - Cancer Biology 2020Quote: ... then the samples were incubated in polymeric horseradish peroxidase (HRP) conjugated secondary antibody (Leica PV6121) for one hour followed by 5 five-minute 1xTBST washes ...
-
bioRxiv - Cancer Biology 2022Quote: ... Antibodies were diluted as follows: p53 (CM5) (1:500, NCL-L-p53-CM5p, Leica Biosystems), p21 (F-5 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Visualization after staining with the antibodies was performed using a high-resolution slide scanner (Leica SCN400FL ...
-
bioRxiv - Pathology 2021Quote: ... 200 uL of anti-mouse secondary antibody (Leica Cat# PV6114, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
-
bioRxiv - Pathology 2021Quote: ... 200 uL of anti-rabbit secondary antibody (Leica Cat# PV6119, diluted 1:125 in PBS) was added to each slide and incubated 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slides were then incubated with HRP-conjugated anti-rabbit secondary antibody (Leica Microsystems, cat. PB6119) for 30 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... IgG (0.5 µg) provided with the kit or 1 µL p53 antibody (CM5, Leica Biosystems) were used for immunoprecipitation ...
-
bioRxiv - Molecular Biology 2024Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary mouse monoclonal antibodies were directed against synaptophysin (1:100, Leica Biosystems, Wetzlar, Germany, #PA0299), neurofilament ...
-
bioRxiv - Cancer Biology 2022Quote: ... slides were incubated with the corresponding secondary antibodies and visualization systems (Novolink Polymer Linker, Leica) conjugated with horseradish peroxidase ...
-
bioRxiv - Developmental Biology 2023Quote: ... The antibody information was listed in Supplementary Table 13 and images were taken from Leica TCS SP8 confocal laser-scanning microscope.
-
bioRxiv - Cell Biology 2023Quote: ... The staining conditions for all primary antibodies were optimized using chromogenic DAB detection (Leica Biosystems). Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences) ...
-
bioRxiv - Biochemistry 2024Quote: ... 10 μg the mouse p53 polyclonal antibody CM5 was used from Leica (#P53-PROTEIN-CM5) or control mouse IgG ...
-
bioRxiv - Microbiology 2024Quote: ... Primary antibodies used for staining included the following: HHV8/LANA (cat# PA0050, Leica, Bannockburn, IL), CD31 (1:200 ...
-
bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
-
bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
-
bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
-
bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
-
bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
-
bioRxiv - Biochemistry 2024Quote: ... The excess liquid was blotted for 4 s prior to plunge-freezing into liquid ethane using a Leica EM GP2 plunger (Leica Microsystems; 95% humidity, 14 °C). Grids were stored in liquid nitrogen prior to data collection ...
-
bioRxiv - Cell Biology 2021Quote: ... Antibody staining was completed using the Bond Polymer Refine IHC protocol and reagents (Leica, Wetzlar, Germany). Slides were counterstained on the Leica Autostainer XL (Leica ...
-
bioRxiv - Genetics 2021Quote: ... Proper secondary antibodies were eventually used and images acquired using a confocal microscope (Leica TCS SP5). The following antibodies were used ...
-
bioRxiv - Microbiology 2021Quote: The following primary antibodies were used: mouse anti-CD45 (Leica Biosystems, NCL-L-LCA, 1/70), mouse anti-CD3 (Leica Biosystems CD3-565-L-CE ...
-
bioRxiv - Cancer Biology 2020Quote: ... The TMA slides were sequentially stained with antibodies specific for CD4 (T cells, 4B12, Leica Biosystems), CD8 (T cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were applied for 60 mins and detected with Polymer Refine Detection Kit (Leica, DS9800). For detection of P63 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies were PowerVision Poly-HRP anti-rabbit or anti-mouse (Leica, PV6114 and PV6119) and developed with DAB.
-
bioRxiv - Cancer Biology 2023Quote: ... Immunohistochemical analyses were performed utilizing ready-to-use Ki67-specific antibody solution (K2, Leica Biosystems, PA0230). Sections were dewaxed ...
-
bioRxiv - Cell Biology 2024Quote: ... Antibodies were diluted as follows: anti-p53 (CM5) (1:500, Leica Biosystems, NCL-L-p53-CM5p), anti-p21 (F-5 ...
-
bioRxiv - Immunology 2024Quote: ... Ready to Use Antibody reagent was added as marker and incubated for 15 min (CD4: Leica Bio systems ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
-
bioRxiv - Immunology 2021Quote: ... Western blot was performed according to the supplier’s instructions using rabbit polyclonal anti-p53 antibody (CM5, Leica) and rabbit-monoclonal anti-vinculin antibody (EPR8185 ...
-
bioRxiv - Microbiology 2021Quote: ... For both antibodies antigen unmasking was undertaken using Heat Induced Epitope Retrieval (HEIR) solution 1 (Leica AR9961) for 30 minutes at 100°C.
-
bioRxiv - Cell Biology 2020Quote: Western blot analysis for human iPSC–derived muscles was performed using antibodies to Dystrophin (NCL-DYS1, Leica), gamma-dystroglycan DAG1 (SC-165997 ...