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Citations for Leica :
451 - 500 of 633 citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The tissues were then embedded in paraffin wax and cut into 4-μm sections with a microtome (RM 2165; Leica Microsystems GmbH). Sections were stained with haematoxylin and hemoglobin (H&E) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: NCAM (Leica Biosystems, NCL-L-CD56-504, 1:50) and MYCN (Novus Biologicals ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-filaggrin primary antibody staining (mouse monoclonal 15C10 from Leica Biosystems, Milton Keynes, UK) was followed by secondary antibody labelling (anti-mouse Alexa 488 ...
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bioRxiv - Neuroscience 2021Quote: ... The Cy3™ - conjugated secondary antibody was detected by a DPSS laser (Leica, Germany) at 561 nm.
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with the secondary antibody (PowerVision Poly-HRP Anti-Mouse IgG, Leica, PV6119) for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Biomarker conditions were as follows: Ki67 (clone MM1 DO-7 mouse monoclonal antibody, Leica) was used at a 1:200 dilution with epitope retrieval solution 2 pre-treatment for 30 min ...
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bioRxiv - Pathology 2023Quote: ... followed by the PowerVision Poly-AP anti-mouse IgG secondary antibody (Cat # PV6110; Leica) for 30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... as well as mouse anti-CD4 monoclonal antibody 4B12 (1:1000, Leica Biosystems; AB_563560) and rabbit anti-IL-17 polyclonal antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue microarray slides were processed as above and incubated with EZH2 antibody (Leica Biosystems) for 1hr by Dr Frances Daley (Pathology Core Facility ...
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bioRxiv - Cell Biology 2024Quote: ... they were coupled with 1 mg/mL of anti-SAG1 antibodies (NCL-Tg, Leica).
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bioRxiv - Cancer Biology 2022Quote: ... The antigen-antibody binding was detected with Leica Bond Polymer Refine Detection (Leica, DS9800) system and the slides were covered with cover glasses ...
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bioRxiv - Cancer Biology 2022Quote: ... the antigen-antibody binding was detected by Bond Polymer Refine Detection (DS9800, Leica Microsystems) and DAB+ chromogen (DAKO ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were incubated with Secondary antibody powervision poly-HRP anti-rabbit IgG (Leica PV6119) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Slides were incubated with Secondary antibody powervision poly-HRP anti-rabbit IgG (Leica PV6119) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibodies were detected using the BOND Polymer Refine Detection System (Leica, DS9800). As positive controls of the IHC ...
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bioRxiv - Immunology 2023Quote: ... Ready-to use secondary antibodies Novolink Post Primary and Novolink Polymer (RE7260-CE, Leica) were used followed by either TSA Cy5 (SAT705A001EA ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (Supplemental Table S1) with a 30M citrate antigen retrieval (Leica ER1 AR9961) were incubated for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 40 μm brain slices from animals perfused with 4% paraformaldehyde (in PBS, pH-7.4) were cut with a cryostat (Leica CM 1800; Leica Canada) and used for immunohistochemistry (IHC) ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixated overnight in 4% paraformaldehyde, followed by sectioning (coronal plane, 50 μm) with a vibratome (VT100S, Leica Biosystems, Wetzlar, Germany). The slices were collected and stored in PB for immunohistochemistry.
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bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 25-µm-thick slices (1:4 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Sections were subjected to IHC using standard methods ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Cell Biology 2021Quote: ... samples were mounted on aluminum SEM stubs and sputter-coated with a 4 nm platinum-palladium layer using a Leica EM ACE600 sputter coater (Leica Microsystems, Illinois, USA). Images were acquired at 2 kV using a Zeiss Sigma 300 SEM (Zeiss ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Pathology 2022Quote: ... in situ hybridization was performed on 12 µm-thick sections of 4% PFA-fixed OCT mounted on charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval (HIER ...
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bioRxiv - Bioengineering 2022Quote: ... Perfusion devices were then fixed with 4% para-formaldehyde and imaged using a 16x immersion lens on SP8 multiphoton microscope (Leica Microsystems, Morrisville, NC). Tumor volumes were quantified by imaging individual tumors post bioprinting
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then embedded in a 4% agarose/1x PBS w/v solution prior to mounting and slicing on a vibratome (Leica VT 1000 S). 40 μm or 60 μm sections containing the pPVN (−0.70 to −1.06 mm relative to Bregma) ...
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bioRxiv - Pathology 2022Quote: ... Pictures of 4-6 different fields per sample were taken under a Leica DM5000 B microscope equipped with a digital CCD (Leica Camera, Wetzlar, Germany).
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bioRxiv - Physiology 2021Quote: ... Fluorescence emission was detected every 526 ms using a Leica SP8 DIVE multiphoton confocal fluorescence imaging system as described above (emission at 460-520 nm for Fluo-4 and at 580-640 nm for Fura Red) (Leica Microsystems, Heidelberg, Germany).
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X). WT ...
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bioRxiv - Plant Biology 2023Quote: ... The fluorescence of the dyes was visualized and recorded 4 hours after the application using a White Light Laser Confocal Microscope (Leica TCS SP8 X).
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bioRxiv - Neuroscience 2022Quote: ... The brain was dissected out followed by post-fixation in 4% paraformaldehyde overnight and sectioned at 50μm using a Leica vibratome (Leica VT1000S, Leica Inc., Germany). Serial floating sections (300μm apart ...
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bioRxiv - Genetics 2022Quote: ... The left femur was decalcified after μCT scanning and were embedded in paraffin and cut into 4-μm sections using a microtome (Leica RM2016, Leica Microsystems). Hematoxylin & eosin (H&E ...
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bioRxiv - Cancer Biology 2024Quote: Multiplexed immunofluorescence imaging: Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were taken and fixed in 4% PFA at RT overnight and 50 μm slices were cut with a vibratome (Leica VT 1000 S). For brain sections from the anterograde and trans-synaptic tracing studies ...
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bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
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bioRxiv - Immunology 2024Quote: ... Brains were placed in 4 °C s-ACSF and sectioned with a vibrotome at 350–400 μm thick (Leica VT1200S; Leica, Nusslock, Germany). Collected slices were then incubated at 32 °C for 1 h in i-ACSF (124 mM/L NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were stored at 4°C and imaged using the 63x oil immersion objective on the Leica TCS SP5 confocal microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Stained slides were placed at 4°C for at least 24 hrs before imaging immunofluorescence using a light microscope (Leica DMi8, Leica Microsystems). To establish relatively quantitative significance ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were sputter-coated with a 4 nm platinum layer using a Leica EM ACE600 sputter coater (Leica Microsystems GmbH, Wetzlar, Germany) and examined using a Zeiss Crossbeam 340 Field-Emission Electron Microscope (Zeiss Microscopy ...
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bioRxiv - Cancer Biology 2024Quote: ... The immunohistochemistry protocol is briefly described as follows: tissue sections (4 μm) were stained in a Leica BOND RX automated stainer (Leica Biosystems Nussloch GmbH). The tissue sections were deparaffinized and rehydrated following the Leica BOND protocol ...
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bioRxiv - Cell Biology 2020Quote: ... neural crest populations were captured at 4 days post-fertilation (dpf) using a Leica Sp8 and analyzed with the Leica Application Suite X (Leica Microsystems, Buffalo Grove, IL) software for image capturing ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were head-fixed in a 1.5 x 4-inch polycarbonate tube under a custom-built fluorescence macroscope consisting of back-to-back 50 mm objective lens (Leica, 0.63x and 1x magnification), separated by a 495nm dichroic mirror (Semrock Inc ...