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Citations for Leica :
451 - 500 of 1513 citations for Ethyl 2 pyrrolidin 2 yl 2 3 dihydro 1 3 thiazole 4 carboxylate hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Tissue sections (∼2 x 2 x 2cm) not needed for pathologic evaluation were fixed in 10% formalin and sliced with a vibratome (Leica VT 1200S) to a thickness of 2mm ...
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bioRxiv - Developmental Biology 2020Quote: ... and serial sections (3 μm thick) were cut using microtome (Leica RM 2125, Germany). The representative sections were stained with hematoxylin and eosin and examined under light microscope (Leica ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed for 3 hours and sectioned on a vibrating microtome (Leica Vibratome, VT1000). Free-floating ...
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bioRxiv - Developmental Biology 2023Quote: ... and serial sections (3 μm thick) were cut using microtome (Leica RM 2125, Germany). The representative sections were stained with hematoxylin and eosin and examined under light microscope (Leica ...
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bioRxiv - Biophysics 2023Quote: ... at 40 °C and for 7 min in 3 % lead citrate (Ultrostain II, Leica) at 20 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The measurements were performed 3 dai using a SP8 laser scanning microscope (Leica Microsystems) with LAS-AF and SymPhoTime software as described (Veerabagu et al. ...
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bioRxiv - Neuroscience 2024Quote: ... coronal brain sections (3 μm) were cut using a Leica RM2016 Microtome (Leica, Germany). The sections were first washed in PBS 3×10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 3 μm were cut with a microtome (Leica RM2255, Leica Microsystems, Germany) on standard glass slides (Waldemar Knittel ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections of 3 μm were cut with a microtome (Leica RM2255, Leica Microsystems, Germany) on standard glass slides (Waldemar Knittel ...
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bioRxiv - Cell Biology 2020Quote: ... cells were loaded with fura-2/AM in the dark (5 μM; 30 min; RT; S1) and imaged with an upright microscope (Leica DMI6000FS, Leica Microsystems) equipped for ratiometric live-cell imaging with a 150W xenon arc lamp ...
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bioRxiv - Microbiology 2021Quote: ... the fast-frozen samples were immersed into a freezing tube containing 2% osmium tetroxide in 100% acetone and placed into the freeze-substitution device (Leica EM AFS, Germany) with the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Videorecordings were acquired at 2 frames per second (fps) using a Grasshopper camera (Point Grey) mounted on a stereomicroscope (Leica MZ6 and MZ7.5). Videos were analysed and animal speed calculated using a custom-written MATLAB software (Zentracker ...
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bioRxiv - Cancer Biology 2022Quote: ... was performed in the VCU Tissue and Data Acquisition and Analysis Core with the Leica Bond RX autostainer using heat-induced epitope retrieval buffer 2 (Leica, EDTA pH 8.0) for 20 min with antibody incubation for 45min ...
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bioRxiv - Plant Biology 2021Quote: ... and the following 2 cm of stem were collected and 100 µm sections were generated using a vibratome (Leica VT1000S, Leica Biosystems GmbH, Germany). These sections were mounted in 0.1 M phosphate buffer at pH 5.8 (close to the physiological pH of plant cell walls ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Immunology 2020Quote: Granulomas were embedded in RPMI containing 2% agarose and sliced into 300–350 μm sections using Leica VT1000 S Vibrating Blade Microtome (Leica Microsystems, Exton, PA). Tissue sections were stained with fluorescently labeled antibodies specific to CD4 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli media in a 0.2 mm deep gold-coated copper carrier prior to high-pressure freezing with a Leica EMpact2 high-pressure freezer (Leica Microsystems, Viena, Austria) [70] ...
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bioRxiv - Immunology 2021Quote: ... Semi-thin (2 μm) sections were cut with glass knifes (KnifeMaster II, LKB Bromma 7800) on an ultramicrotome (Leica Ultracut, Leica Microsystems, Wetzlar, Germany), stained firstly with 1% toluidine blue (VWR 34187.185 ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were seeded at 2000 cells/ml (total volume 2 ml) before starting the experiment at 37ºC supplemented with 5% CO2 in a live cell chamber (Leica DMI6000B with PEKON stage). Cells that attached and spread during the first two hours were used to quantify the changes in traction stresses during adhesion ...
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bioRxiv - Cell Biology 2023Quote: ... The polymerized cells were sectioned to a 2 μm depth from the block face using a Leica EM ARTOS 3D ultramicrotome (Leica Microsystems, Wetzlar, Germany) and a diamond knife (DiATOME ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030, Leica Microsystems, Wetzlar, Germany). Using a scoring system bleeding intensity was evaluated in vaginal smears by microscopy ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately eight semi-thin sections (2 μm) from one block were cut on an ultramicrotome with a glass knife (Leica, Reichert Ultracut UTC). These were stained with 1% toluidine blue in 1% Borax and evaluated by light microscopy to locate areas with adequate number of cells for further processing ...
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bioRxiv - Bioengineering 2022Quote: ... The homogeneity of BMP-2 loading inside the film-coated 3D mini-scaffolds was assessed using a BMP-2 loading solution containing 5% of BMP-2Rhod and a fluorescence macroscope (Macrofluo Z16 Apo, Leica Microsystems, Wetzlar, Germany) with a 0.8X objective ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Neuroscience 2024Quote: ... the hemisphere was gelatine-embedded and coronally sectioned between anteroposterior coordinates -2 mm and -4.8 mm (dorsal hippocampus) with a cryostat (Leica CM3050 S, Leica Biosystems). Sections of 40 µM were placed on anti-freeze solution [30% glycerol ...
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bioRxiv - Microbiology 2022Quote: ... covered with the flat side of a type B 3 mm aluminum platelet (Leica Microsystems), and was vitrified by high-pressure freezing using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were blocked on 3% agarose and sectioned using a VT1200 S vibratome (Leica Biosystems), to 40 μm sections ...
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bioRxiv - Cell Biology 2022Quote: ... intact gametocysts bathed in 3% BSA were subjected to high-pressure freezing (HPM100, Leica Microsystems), before freeze-substitution using a temperature-controlled chamber (AFS ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with 3% isoflurane and placed in a stereotaxic frame (Leica Microsystems Inc). For the craniotomy (centered at A/P −4.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... FFPE samples were sectioned in slices with 3 μm thickness (Microtome Leica RM135, Newcastle, UK) and mounted on a glass slide (SuperFrost Plus ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were sectioned at 3 μm thickness on a LeicaRM2145 microtome (Leica Microsystems, Nussloch, Germany) and stained in Richardson solution (1% methylene blue ...
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bioRxiv - Genetics 2022Quote: ... brains were embedded in 3% low-melting agarose and cut with a vibratome (VT1200S, Leica). 100µm thick sections were mounted on gelatine-coated slides and allow to dry for two days before staining ...
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bioRxiv - Microbiology 2022Quote: ... covered with the flat side of a 3-mm type B aluminum plate (Leica Microsystems) and vitrified by high-pressure freezing in a HPM100 system (Leica Microsystems) ...
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bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
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bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
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bioRxiv - Neuroscience 2023Quote: ... TA muscles were embedded in 3% agarose in PBS and sectioned on a vibratome (Leica) at 100 μm or 200 μm ...
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bioRxiv - Bioengineering 2023Quote: SNO were embedded in 3% low melting agarose and sectioned using Leica VT1000S vibratome (Leica) into slices of 400 μm diameter ...
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bioRxiv - Microbiology 2023Quote: ... covered with the flat side of a 3-mm type-B aluminum platelet (Leica Microsystems). The sample was vitrified by high-pressure freezing using an HPM100 system (Leica Microsystems ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections (3 µm thickness) were obtained using a microtome (HM 335E; Leica Microsystems) and stained with 0.2% toluidine blue O for observation under a BX51 light microscope (OLYMPUS) ...
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bioRxiv - Microbiology 2024Quote: ... covered with the flat side of a 3-mm type-B aluminum platelet (Leica Microsystems). The samples were vitrified by high-pressure freezing using an HPM100 system (Leica Microsystems ...
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bioRxiv - Neuroscience 2024Quote: ... washed with PBS and freshly sliced using a vibratome (Leica VT1000S, vibration 10, speed 3) at 50 μm ...
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bioRxiv - Cell Biology 2024Quote: ... The mixture was transferred into the 3 mm specimens carriers (Leica Microsystems Inc, Vienna, Austria), and then was high-pressure frozen with a Leica EM HPM100 High Pressure Freezer (Leica Microsystems Inc ...
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bioRxiv - Neuroscience 2020Quote: ... and resilient (n=12) mice from experiment 2 were sliced into 300 μm coronal slices on a Leica CM3050 S Research Cryostat (Leica Biosystems, Amsterdam, the Netherlands), with a chamber temperature of −12 °C and an object temperature of −10 °C ...
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bioRxiv - Plant Biology 2022Quote: ... the semi-thin sections (thickness 2 μm) were cut by an automatic semi-thin rotary cutting machine (LEICA RM2265, Leica Microsystems Co., Ltd., Germany) and triangular glass knife ...
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bioRxiv - Neuroscience 2024Quote: ... Trimmed except for the cerebrum and embedded in 2% agarose gel to make 100 µm thick sagittal sections using a microtome (VT1200S; Leica Microsystems GmbH, Wetzlar, Germany). Sections were stored at 4 °C in 1 x PBS(- ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were incubated in 3% hydrogen peroxide (Bond Polymer Refine Detection Kit DS9800, Leica Biosystems) for 15 mins to block endogenous peroxidases ...
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bioRxiv - Evolutionary Biology 2021Quote: ... embedded in 3% LM-agarose (Roth) and cut 250 µm-thick with a vibratome (Leica VT1000S). Slices were then transferred on Millicell inserts (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: Broad-band autofluorescence was acquired from sections cut at 3 μm using X20 objective (Leica DM550B). Sections were excited at 458 nm and fluorescence emission captured above 475 nm ...