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Citations for Origene Technologies :
451 - 500 of 1832 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 0.75 μl of the forward and reverse primer (OriGene, HP205798), and 10.75 μl of RNase-free water were added to each reaction well followed by 1μl of cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Stable TFEB knockdown was achieved by transfecting TFEB-specific shRNA cloned in pRFP-C-RS plasmid (OriGene RNAi, 0513). The same plasmid containing non-specific shRNA was used as a control (OriGene RNAi ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: goat anti-mCherry (Origene, AB0040-200, 1:500), mouse anti-His (Dianova ...
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bioRxiv - Cell Biology 2023Quote: ... anti-cytokeratin 8 (Origene BP5074), anti-Ki67-FITC (eBioscience 11-5698-90) ...
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bioRxiv - Neuroscience 2023Quote: ... the following antibodies were purchased: goat anti-tdTomato (RRID:AB_2722750, Origene, Rockville, MD, USA, #AB8181-200), rabbit anti-St8sia1 (RRID:AB_1857534 ...
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bioRxiv - Cell Biology 2023Quote: ... The manufacturer’s protocol was used for viral production by mixing pLenti-C-TAZ-mGFP-P2A-Puro with packaging plasmids and transfection reagent from the Lenti-vpak packaging kit (OriGene, Cat#TR30037). The mixture was transfected into HEK293T cells to generate pseudoviral particles ...
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bioRxiv - Microbiology 2023Quote: ... pCMV6-MORC2-myc-DDK have been purchased from Origene. pCMV6-MORC2-myc-DDK vector expresses MORC2 isoform 1 of 1032 amino acids (NCBI Reference Sequence ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length cDNA for Human KCNT1 Transcript 1 NM_020822.1 (Origene Technologies Inc, Rockville, MD, USA) was mutagenised to induce point mutations ...
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bioRxiv - Physiology 2023Quote: Human AgRP (Origene CAT#: RC217144) was cloned into CAG-NLS-GFP (Addgene# ...
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bioRxiv - Neuroscience 2023Quote: ... Human Adgrd1 cDNA was obtained from Origene (Cat: PS100001). Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... ZBTB7A overexpression plasmids (Origene Cat. #RC222759) were cloned into either a Lentiviral CMV-driven construct for use in cell culture experiments (shown in Figure S2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and on the next day transfected using TurboFectin 8.0 (OriGene) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP was cloned at the C terminus of the coding sequence of Pitx2 isoform 1 (Origene; MR227617). Lentivirus particles were generated as previously described 69 ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with goat anti-tdTomato antibody (1:300, Origene, # AB8181-200), rabbit anti-St8sia1 (GD3S ...
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bioRxiv - Neuroscience 2023Quote: ... was then used with an anti-FKBP5 antibody (OriGene, Rockville, MD, USA) at a 1 in 80 dilution ...
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bioRxiv - Pathology 2023Quote: Human astrocytes used co-culture were first transduced with lentiviruses carrying GFP (LentiORF control particles of pLenti-C-mGFP-P2A-Puro Origene™ cat# PS100093V), CLU (Lenti ORF particles ...
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bioRxiv - Physiology 2023Quote: ... by using siTran2.0 transfection reagent (#TT320001, Origene) and 50 nM scrambled siRNA with similar median GC content was used as siRNA control (#12935300 ...
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bioRxiv - Cell Biology 2023Quote: ... human FBXO38 cDNA (Origene #RC204380) was cloned into pcDNA3.1 containing N-terminal twinStrepII- FLAG-tag (NSF) ...
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bioRxiv - Cell Biology 2023Quote: ... the plasmid K07 (OriGene Technologies, Inc., Rockville, USA), which lacked homology with any known mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse OSM expression plasmid (MR226014) was purchased from Origene. Mouse STAT3-Y705F plasmid was kindly provided by Prof ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: B16F10 cells were transfected with Rab27a-mouse shRNA and scramble RNA lentiviral particles purchased from Origene according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse TMEM87A -transcript variant 1 (NM_173734; MC201598) were purchased from Origene. The coding sequences of these genes were PCR amplified and then subcloned into CMV-pIRES2-DsRed/iRFP vector using the SalI/BamHI restriction enzyme sites or CMV-EGFP-N1 vector using the EcoRI/AgeI restriction enzyme sites using the cloning kit (EZ-Fusion™ HT Cloning core Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Construct expressing murine Src was obtained from Origene.
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bioRxiv - Microbiology 2023Quote: ... and FcER1G (SC117594, OriGene Technologies, Inc, MD) constructs (5 μg of each) ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human and mouse SLFN14 (Origene, RC226257 and MR225976) were expressed from pCMV6-Entry ...
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bioRxiv - Molecular Biology 2023Quote: ... pCMV6-Fam122a-Myc-DDK was purchased from Origene. pCMV6-AN-DDK-Fam122a was generated using SgfI/MluI restriction sites from the precision shuttle system (pCMV6-AN-DDK-Pol Iota-A kind gift from Roger Woodgate Addgene #131228) ...
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bioRxiv - Genetics 2023Quote: SCN1A splicing reporter plasmids (5ug) were transfected in HEK293T cells using TurboFectin 8.0 (Origene #TF81001). After twenty-four hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Human C-terminally Myc-FLAG-tagged ZDHHC20 (C-FLAG-D20) was purchased from Origene Technologies ...
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bioRxiv - Cell Biology 2023Quote: Transfection of cells was performed transiently with plasmid DNA using Turbofectin 8.0 (PN: TF81001, Origene), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... transient or tetracyclin-inducible expression of human HEATR5B with an N-terminal GFP tag in human cells) were cloned by Gibson assembly with the full-length human HEATR5B open reading frame (derived from plasmid RC22610 (Origene)) and either pcDNA3.1-eGFP-linker or pcDNA5-FRT/TO-eGFP-linker plasmids (coding for eGFP and a GGSGGSGG linker ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti-BAG5 (CF810618) was purchased from OriGene Technologies ...
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bioRxiv - Biochemistry 2023Quote: Human cell derived recombinant eIF2A-FLAG was expressed in HEK293T cells obtained commercially (OriGene # TP304303) and buffer exchanged into Protein Storage Buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... virus-containing supernatant was collected and concentrated using Lenti-Concentrator (OriGene), for minimum 2h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product and the plasmid pLenti-C-mGFP (# PS100071 OriGene Technologies, Rockville, MD, USA) were digested with Asc1 (#R0558L Bioconcept ...
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bioRxiv - Physiology 2023Quote: ... The SIK3-Flag plasmid was constructed by subcloning of a 3xFlag tag to replace the Myc-DDK tag in the mSIK3-Myc-DDK plasmid (MR211912, OriGene). SIK3-Flag-ΔPKA (ΔPKA ...
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bioRxiv - Biochemistry 2023Quote: ... This ORF was then subcloned into a hygromycin resistance-containing pCMV6 entry vector (OriGene, CAT PS100024) and used to generate an LRPPRC-KO cell line reconstituted with a wild-type LRPPRC gene as reported 27 ...
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bioRxiv - Biochemistry 2023Quote: ... a Myc- DDK tagged LRPPRC ORF plasmid was obtained from OriGene (CAT: RC216747). This ORF was then subcloned into a hygromycin resistance-containing pCMV6 entry vector (OriGene ...
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bioRxiv - Physiology 2023Quote: ... The NAA10-Myc plasmid was constructed by subcloning of a Myc-His tag to replace the Myc-DDK tag in the hNAA10-Myc-DDK plasmid (RC201354, OriGene). The SIK3-Flag plasmid was constructed by subcloning of a 3xFlag tag to replace the Myc-DDK tag in the mSIK3-Myc-DDK plasmid (MR211912 ...
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bioRxiv - Cancer Biology 2023Quote: ... Each plate contains 48 samples from patients with cancer whose clinical pathological features are freely available at the following link from Origene: https://www.origene.com/catalog/tissues/tissuescan/hcrt104/tissuescan-colon-cancer-cdna-array-iv ...
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bioRxiv - Cancer Biology 2023Quote: ... Myc-DDK tagged human CD2-associated protein (RC210191) was purchased from Origene Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The Colon Cancer Tissue cDNA Array IV panel (HCRT104) was purchased from Origene Technologies (Origene Technologies Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... V5-TKS4 or/and MYC-CD2AP (Myc-DDK-Tagged CD2AP clone) (CAT#: RC210191, Origene Technologies Inc., Rockville, MD) plasmids were transfected using X-tremeGENE Hp DNA Transfection Reagent (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... was purchased from Origene Technologies (Origene Technologies Inc., Rockville, MD). Each plate contains 48 samples from patients with cancer whose clinical pathological features are freely available at the following link from Origene ...
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bioRxiv - Biophysics 2023Quote: ... or N1β with an N-terminal tGFP tag (custom plasmid from Origene) by Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2023Quote: ... WIPI-4-TurboGFP (Origene; WDR45-tGFP transcript variant 1 ...
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bioRxiv - Immunology 2023Quote: ... empty pCMV6 vector (OriGene) was transfected into the cells ...
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bioRxiv - Immunology 2023Quote: ... 10 μg of pCMV6 encoding ANKRD22 gene (OriGene, Rockville, MD, USA) was incubated with 30 μL of TransIT-293 in 600 uL of OPT-MEM for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The shRNA against PLD2 was provided by Origene.
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bioRxiv - Developmental Biology 2023Quote: ... we amplified the Sfrp2 coding sequence from an Sfrp2 (NM_009144) Mouse Tagged ORF Clone (Origene CAT#: MR204070) using CloneAmp™ HiFi PCR Premix (Takara 639298 ...
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bioRxiv - Biophysics 2023Quote: ... flag-tagged mTHSD7A construct serving as the PCR template (Origene).