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Citations for Tocris :
501 - 546 of 546 citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Mechanically dissociated cells in accutase were diluted with 4x volume of StemFlex with 10uM Y-27632 (ROCK inhibitor, Tocris, #1254) and manually counted with Trypan Blue ...
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bioRxiv - Systems Biology 2023Quote: These stable cell lines were split into separate wells of a 24-well plate and either treated with doxycycline (Tocris) or left untreated until analyzed by flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with quercetin for approximately 24 hours with DMSO used as a negative control and ActD (Tocris 1229) used as a positive control ...
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bioRxiv - Cancer Biology 2023Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... Accutase-dissociated single cells are FACS-sorted for the expression of the respective fluorescent marker protein and either directly used for bulk NGS based validation of the desired genome modification or subsequently plated at clonal density (250 to 350 cells/cm2) on MEFs in hESC media supplemented with 10 µm ROCK inhibitor (Y27632, ToCris) for the first 24 hr ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies were dissociated and plated into pre-coated 6-well Aggrewell 800 plates at a concentration of 18M cells per well in hESC media with 10 µM Rock Inhibitor (ToCris). The next day (Day 1) ...
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bioRxiv - Immunology 2019Quote: ... Cell were then washed and rested overnight in complete RPMI medium without or with JAK kinase inhibitor CP-690550 (Tocris Biosciences), and stimulated for 4 hours with 100U/ml of IL-2 ...
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bioRxiv - Biochemistry 2020Quote: ... and plated at 50,000 cells per plate in 2 mL of E8® media supplemented with 2 µL of 1000x Rho/Rock inhibitor Y27632 (Tocris). Prior to incubation with the appropriate peptide ...
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bioRxiv - Neuroscience 2021Quote: ... cells were whole-cell voltage-clamped at −40mV and recorded in the presence of the GABAAR antagonist Gabazine (10 μM, Tocris 1262). N-methyl-D-aspartic acid (NMDA ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were seeded on Matrigel-coated plates or coverslips at a density of 1.1×105 cells/cm² in mTeSR1 medium supplemented with 10 μM of ROCK inhibitor Y-27632 (Ri; Tocris, ref. 1254). To induce MES differentiation ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2022Quote: Macrophages were plated in a 12-well plate at 106 cells per well and treated with 10 μM E64 (Tocris, 5208) or 20 μM Z-Phe-Ala-FMK cathepsin B inhibitor (Santa Cruz ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...
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bioRxiv - Immunology 2020Quote: ... anti-IgM/CpG-activated or non-activated B cells were incubated with or without reserpine (5 μM, Tocris, catalogue number: 2747), an inhibitor of VMAT-1 & VMAT-2 for 45min ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Human ePSCs were thawed at 37°C and plated on top of the feeder cells in EPSCM with 10 µM Y-27632 dihydrochloride (Tocris, 1254) for 48 h ...
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Bioengineering 2023Quote: ... and then seeded at a density of 3.8 × 10⁴ cells per well in 1% Geltrex-coated 24-well cell culture plates (30024, SPL Life Sciences) in mTeSR1 supplemented with Y-27632 dihydrochloride (10 μM) (1254, Tocris Bioscience). The medium was replaced with mTeSR1 the following day ...
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 µM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for ≤ 10 passages after thawing.
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bioRxiv - Cancer Biology 2023Quote: ... 1000 cells/well were seeded and pre-treated with SCFAs (SP or SB) for 48 hours followed by cisplatin (Tocris, 2251) treatment in a dose dependent manner for 72 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting after treatment of the cells with 1 µM dTAG-7 (Tocris, 6912) for 3 h ...
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bioRxiv - Genetics 2023Quote: ... and resuspended in stem cell media supplemented with 10 μM Y-27632 dihydrochloride (Rho kinase [ROCK] inhibitor; Tocris; cat. no. 1254). hiPSCs were used for no more than 10 passages after thawing ...
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bioRxiv - Neuroscience 2023Quote: ... 12-well plates at 7-8 x 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y- 27632; Tocris, 1254). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... hiPSCs were then differentiated to neural precursor cells by dual SMAD inhibition using 0.1 mM LDN193189 (Biotechne/Tocris Cat. No. 6053) and 10 mM SB431542 (Biotechne/Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... then treated for an additional 24 h in the presence (synchronised in G2 cell cycle phase) or absence (asynchronised) of 9 μM CDK1 inhibitor RO-3306 (CDK1i; Tocris Bioscience) prior to collection.
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bioRxiv - Neuroscience 2023Quote: iPSCs were replated at a density of 2 x 104 cells/cm2 in mTeSR medium containing 10 µM Rho kinase inhibitor (Y-27632, Tocris #5849), which was removed after 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... all buffers and solutions used during the process (from animal perfusion to sorted cells flash freezing) were supplemented with a cocktail composed of Actinomycin D (3μM, Tocris #1229/10), Anisomycin (100 μM ...
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bioRxiv - Cell Biology 2024Quote: THP-1 cells were differentiated in complete media with phorbol 12-myristate-13-acetate (PMA; 100 nM) (cat no. 1201/1; Tocris, UK) for 72 hr ...
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bioRxiv - Immunology 2024Quote: ... The following inhibitors were added to the cells for 24 hours before harvesting: the NF-κB inhibitor JSH23 (2 µM, Tocris Bioscience), STING inhibitor H151 (5 µM ...
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bioRxiv - Neuroscience 2022Quote: ... 200 nM, 90 min application for the experiments on transfected cells; 120 min application for electrophysiological recordings in oocytes, Tocris; Cat. #5756) [18 ...
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bioRxiv - Neuroscience 2021Quote: ... Whole cell currents were recorded (see electrophysiology below) with synaptic transmission and action potentials blocked by CPPene (10 µM, Tocris bioscience; 1265), NBQX (10 μM ...
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bioRxiv - Microbiology 2021Quote: ... iAT2 cells were cultured as 2D cultures on Matrigel-coated plates in CK+DCI medium + 10 µM Y-27632 (Tocris, Cat#1254) for 48 h before switching to CK+DCI medium on day 3.
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bioRxiv - Genomics 2022Quote: ... iPS cells were grown to 80– 90% confluence and changed into CM Media supplemented with 3 μM CHIR99021 (Tocris Biosciences, Bristol, UK) for 2 days ...
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bioRxiv - Neuroscience 2020Quote: ... was applied at the end of each experiment as a pharmacological test of DA neuron ientity.25 Whole-cell somatodendritic nAChR-mediated currents were evoked in the presence of tetrodotoxin (TTX) (0.5 μM, Tocris Bioscience, Bristol, UK) by locally applying acetylcholine (ACh ...
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bioRxiv - Developmental Biology 2021Quote: ... confluent hiPSC clones cultured in mTeSR were dissociated with Accutase and plated at 50,000 cells/well of a 6well plate in mTeSR with 10uM Y-27632 (Tocris Bioscience, Bristol, UK) for one day ...
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bioRxiv - Microbiology 2021Quote: ... iATII cells were cultured as 2D cultures on Matrigel-coated plates in CK+DCI medium + 10 µM Y-27632 (Tocris, Cat#1254) for 48h before switching to CK+DCI medium on day 3.
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... These cells were then directed to become proepicardial cells by the addition of retinoic acid and WNT CHIR 99021 followed by TGFβ inhibition (SB431542, Tocris Bioscience 1614). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines were maintained in mTeSR1 Plus Medium (Stemcell Technologies 100-0276 and 1 µM of Rock inhibitor (RI) (Tocris 1254). Differentiation was achieved by culturing the cells for 16 days in N2B27 media ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubating the cells for 30 min in freshly-prepared blocking solution (1X TTBS, 1% bovine serum albumin (Tocris Bioscience, UK) and 0.25% Triton-X100) ...
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bioRxiv - Developmental Biology 2021Quote: ... dissociation and seeded at a density of 5× 104 cells per cm2 in mTeSR1 supplemented with 10μM Y-27362 dihydrochloride (Rocki; Tocris Bioscience, cat. no. 1254).
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bioRxiv - Neuroscience 2022Quote: ... Disassociated cells were pre-plated as reported earlier20 at a density of 200,000 cells/cm2 supplemented with 10 μM Y- 27632 (catalog# 1254, Tocris Bioscience, Bristol, United Kingdom) on Matrigel-coated plates and differentiation started when confluent ...
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bioRxiv - Neuroscience 2022Quote: ... dissociated hiPSCs were plated at 1×104 cells/cm2 into 6-well plates in the presence of 10 µM ROCK inhibitor (Y-27632, Tocris Bioscience, Bristol, UK). Cortical and hypothalamic differentiation took place on a substrate of geltrex (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... for 18-30 minutes and reseeding them into Matrigel (BD Bioscience #354277)-coated 6-well plates at 1 x 105 cells/cm2 in cortical differentiation medium containing 10 µM Rock-inhibitor (Tocris, Minneapolis, MN, USA #1254), which was removed after 24 hours ...